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题名: Dietary pantothenic acid deficiency and excess depress the growth, intestinal mucosal immune and physical functions by regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)
作者: Li, Li1; Feng, Lin1, 2, 3; Jiang, Wei-Dan1, 2, 3; Jiang, Jun1, 2, 3; Wu, Pei1, 2, 3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1, 2, 3; Liu, Yang1, 2, 3
关键词: Pantothenic acid ; Grass carp ; Mucosal immune ; Antioxidant capability ; Tight junction protein ; mRNA level ; Intestine
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2015-08-01
DOI: 10.1016/j.fsi.2015.04.030
卷: 45, 期:2, 页:399-413
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: This study investigated the effects of dietary pantothenic acid (PA) on the growth, intestinal mucosal immune and physical barrier, and relative mRNA levels of signaling molecules in the intestine of grass carp (Ctenopharyngodon idella). A total of 540 grass carp (253.44 +/- 0.69 g) were fed six diets with graded levels of PA (PA1, PA15, PA30, PA45, PA60 and PA75 diets) for 8 weeks. The results indicated that compared with PA deficiency (PA1 diet) and excess (PA75 diet) groups, optimal PA supplementation increased (P < 0.05): (1) percent weight gain (PWG), feed intake and feed efficiency; (2) lysozyme activity, complement 3 content, liver-expressed antimicrobial peptide 2 and hepcidin, interleukin 10, transforming growth factor beta 1 and inhibitor of kappa B alpha mRNA levels in some intestinal segments; (3) activities and mRNA levels of copper/zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferases and glutathione reductase, and NF-E2-related factor 2 (Nrf2) mRNA level in the whole intestine; (4) Claudin b, Claudin 3, Claudin c, Occludin and ZO-1 mRNA levels in some intestinal segments of grass carp. Conversely, optimal PA supplementation decreased (P < 0.05): (1) tumor necrosis factor alpha, interleukin 1 beta, interferon gamma 2, interleukin 8, nuclear factor kappa B P65 (NF-kappa B P65), I kappa B kinase alpha, I kappa B kinase beta, I kappa B kinase gamma and target of rapamycin (TOR) mRNA expression levels in some intestinal segments; (2) reactive oxygen species, malondialdehyde and protein carbonyl contents, and Kelch-like ECH-associating protein 1a, Kelch-like ECH-associating protein 1b in the intestine; (3) Claudin 12, Claudin 15a and myosin light-chain kinase (MLCK) mRNA levels in some intestinal segments of grass carp. In conclusion, optimum PA promoted growth, intestinal mucosal immune and physical function, as well as regulated mRNA levels of signaling molecules NF-kappa B P65, TOR, Nrf2 and MLCK in grass carp intestine. Based on the quadratic regression analysis of PWG and intestinal lysozyme activity, the optimal PA levels in grass carp (253.44-745.25 g) were estimated to be 37.73 mg/kg and 4138 mg/kg diet, respectively. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: SALMON SALMO-SALAR ; FED MANNAN OLIGOSACCHARIDES ; TROUT ONCORHYNCHUS-MYKISS ; TIGHT JUNCTION STRUCTURE ; BARRIER FUNCTION ; ATLANTIC SALMON ; GENE-EXPRESSION ; JUVENILE JIAN ; VAR. JIAN ; ENZYME-ACTIVITIES
语种: 英语
WOS记录号: WOS:000358626800023
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25964
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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