IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Dietary pantothenic acid deficiency and excess depress the growth, intestinal mucosal immune and physical functions by regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)
Li, Li1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Jiang, Jun1,2,3; Wu, Pei1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Liu, Yang1,2,3
2015-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume45Issue:2Pages:399-413
AbstractThis study investigated the effects of dietary pantothenic acid (PA) on the growth, intestinal mucosal immune and physical barrier, and relative mRNA levels of signaling molecules in the intestine of grass carp (Ctenopharyngodon idella). A total of 540 grass carp (253.44 +/- 0.69 g) were fed six diets with graded levels of PA (PA1, PA15, PA30, PA45, PA60 and PA75 diets) for 8 weeks. The results indicated that compared with PA deficiency (PA1 diet) and excess (PA75 diet) groups, optimal PA supplementation increased (P < 0.05): (1) percent weight gain (PWG), feed intake and feed efficiency; (2) lysozyme activity, complement 3 content, liver-expressed antimicrobial peptide 2 and hepcidin, interleukin 10, transforming growth factor beta 1 and inhibitor of kappa B alpha mRNA levels in some intestinal segments; (3) activities and mRNA levels of copper/zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferases and glutathione reductase, and NF-E2-related factor 2 (Nrf2) mRNA level in the whole intestine; (4) Claudin b, Claudin 3, Claudin c, Occludin and ZO-1 mRNA levels in some intestinal segments of grass carp. Conversely, optimal PA supplementation decreased (P < 0.05): (1) tumor necrosis factor alpha, interleukin 1 beta, interferon gamma 2, interleukin 8, nuclear factor kappa B P65 (NF-kappa B P65), I kappa B kinase alpha, I kappa B kinase beta, I kappa B kinase gamma and target of rapamycin (TOR) mRNA expression levels in some intestinal segments; (2) reactive oxygen species, malondialdehyde and protein carbonyl contents, and Kelch-like ECH-associating protein 1a, Kelch-like ECH-associating protein 1b in the intestine; (3) Claudin 12, Claudin 15a and myosin light-chain kinase (MLCK) mRNA levels in some intestinal segments of grass carp. In conclusion, optimum PA promoted growth, intestinal mucosal immune and physical function, as well as regulated mRNA levels of signaling molecules NF-kappa B P65, TOR, Nrf2 and MLCK in grass carp intestine. Based on the quadratic regression analysis of PWG and intestinal lysozyme activity, the optimal PA levels in grass carp (253.44-745.25 g) were estimated to be 37.73 mg/kg and 4138 mg/kg diet, respectively. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordPantothenic Acid Grass Carp Mucosal Immune Antioxidant Capability Tight Junction Protein Mrna Level Intestine
DOI10.1016/j.fsi.2015.04.030
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000358626800023
WOS KeywordSALMON SALMO-SALAR ; FED MANNAN OLIGOSACCHARIDES ; TROUT ONCORHYNCHUS-MYKISS ; TIGHT JUNCTION STRUCTURE ; BARRIER FUNCTION ; ATLANTIC SALMON ; GENE-EXPRESSION ; JUVENILE JIAN ; VAR. JIAN ; ENZYME-ACTIVITIES
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25964
Collection水生生物分子与细胞生物学研究中心_期刊论文
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Li,Feng, Lin,Jiang, Wei-Dan,et al. Dietary pantothenic acid deficiency and excess depress the growth, intestinal mucosal immune and physical functions by regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):399-413.
APA Li, Li.,Feng, Lin.,Jiang, Wei-Dan.,Jiang, Jun.,Wu, Pei.,...&Liu, Yang.(2015).Dietary pantothenic acid deficiency and excess depress the growth, intestinal mucosal immune and physical functions by regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,45(2),399-413.
MLA Li, Li,et al."Dietary pantothenic acid deficiency and excess depress the growth, intestinal mucosal immune and physical functions by regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):399-413.
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