Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency: Regulation of tight junction protein transcript, antioxidant status and apoptosis-related gene expression
Feng, Lin1,2,3; Luo, Jian-Bo1; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Wu, Pei1,2,3; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3
2015-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume45Issue:2Pages:239-249
AbstractThis study investigated the effects of dietary valine on tight junction protein transcription, antioxidant status and apoptosis on grass carp gills (Ctenopharyngodon idella). Fish were fed six different experimental diets containing graded levels of valine (4.3, 8.0, 10.6, 13.1, 16.7, 19.1 g/kg). The results indicated that valine deficiency decreased Claudin b, Claudin 3, Occludin and ZO-1 transcription and increased Claudin 15 expression in the fish gill (P < 0.05). These effects were partly due to the down-regulation of interleukin 10 (IL-10), transforming growth factor 131 (TGF-beta 1) and I kappa B alpha and the up-regulation of relative mRNA expression of interleukin 1 beta (IL-1 beta), interleukin 8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and nuclear factor kappa B P65 (NF-kappa B P65) (P < 0.05). However, valine deficiency and valine supplementation did not have a significant effect on Claudin c and Claudin 12 expression in grass carp gills (P > 0.05). Valine deficiency also disrupted antioxidant status in the gill by decreasing anti-superoxide radicals and hydroxyl radical capacity, glutathione contents and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) (P < 0.05). These results may be ascribed to the down-regulation of NF-E2-related factor 2 (Nrf2), target of rapamycin (TOR) and ribosomal protein S6 kinase 1 (S6K1) and the up-regulation of Kelch-like-ECH-associated protein 1 (Keap1) (P < 0.05). Additionally, valine deficiency induced DNA fragmentation via the up-regulation of Caspase 3, Caspase 8 and Caspase 9 expressions (P < 0.05). These results may be ascribed to the improvement in ROS levels in the fish gill (P < 0.05). Taken together, the results showed that valine deficiency impaired the structural integrity of fish gill by disrupted fish antioxidant defenses and regulating the expression of tight junction protein, cytokines, antioxidant enzymes, NF-kappa B p65, I kappa B alpha, TOR, Nrf2, Keap1 and apoptosis-related genes in the fish gill. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordValine Deficiency Tight Junction Protein Antioxidant Defense Signaling Molecule Apoptosis
DOI10.1016/j.fsi.2015.04.023
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordNF-KAPPA-B ; MUCOSAL IMMUNE-SYSTEM ; SALMON SALMO-SALAR ; TROUT ONCORHYNCHUS-MYKISS ; FATTY-ACID-COMPOSITION ; CHAIN AMINO-ACIDS ; OXIDATIVE STRESS ; CYPRINUS-CARPIO ; STENOHALINE GOLDFISH ; AQUATIC ORGANISMS
WOS IDWOS:000358626800005
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25963
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Feng, Lin,Luo, Jian-Bo,Jiang, Wei-Dan,et al. Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency: Regulation of tight junction protein transcript, antioxidant status and apoptosis-related gene expression[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(2):239-249.
APA Feng, Lin.,Luo, Jian-Bo.,Jiang, Wei-Dan.,Liu, Yang.,Wu, Pei.,...&Zhou, Xiao-Qiu.(2015).Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency: Regulation of tight junction protein transcript, antioxidant status and apoptosis-related gene expression.FISH & SHELLFISH IMMUNOLOGY,45(2),239-249.
MLA Feng, Lin,et al."Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency: Regulation of tight junction protein transcript, antioxidant status and apoptosis-related gene expression".FISH & SHELLFISH IMMUNOLOGY 45.2(2015):239-249.
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