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题名: Changes in barrier health status of the gill for grass carp (Ctenopharyngodon idella) during valine deficiency: Regulation of tight junction protein transcript, antioxidant status and apoptosis-related gene expression
作者: Feng, Lin1, 2, 3; Luo, Jian-Bo1; Jiang, Wei-Dan1, 2, 3; Liu, Yang1, 2, 3; Wu, Pei1, 2, 3; Jiang, Jun1, 2, 3; Kuang, Sheng-Yao4; Tang, Ling4; Zhang, Yong-An5; Zhou, Xiao-Qiu1, 2, 3
关键词: Valine deficiency ; Tight junction protein ; Antioxidant defense ; Signaling molecule ; Apoptosis
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2015-08-01
DOI: 10.1016/j.fsi.2015.04.023
卷: 45, 期:2, 页:239-249
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
英文摘要: This study investigated the effects of dietary valine on tight junction protein transcription, antioxidant status and apoptosis on grass carp gills (Ctenopharyngodon idella). Fish were fed six different experimental diets containing graded levels of valine (4.3, 8.0, 10.6, 13.1, 16.7, 19.1 g/kg). The results indicated that valine deficiency decreased Claudin b, Claudin 3, Occludin and ZO-1 transcription and increased Claudin 15 expression in the fish gill (P < 0.05). These effects were partly due to the down-regulation of interleukin 10 (IL-10), transforming growth factor 131 (TGF-beta 1) and I kappa B alpha and the up-regulation of relative mRNA expression of interleukin 1 beta (IL-1 beta), interleukin 8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and nuclear factor kappa B P65 (NF-kappa B P65) (P < 0.05). However, valine deficiency and valine supplementation did not have a significant effect on Claudin c and Claudin 12 expression in grass carp gills (P > 0.05). Valine deficiency also disrupted antioxidant status in the gill by decreasing anti-superoxide radicals and hydroxyl radical capacity, glutathione contents and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) (P < 0.05). These results may be ascribed to the down-regulation of NF-E2-related factor 2 (Nrf2), target of rapamycin (TOR) and ribosomal protein S6 kinase 1 (S6K1) and the up-regulation of Kelch-like-ECH-associated protein 1 (Keap1) (P < 0.05). Additionally, valine deficiency induced DNA fragmentation via the up-regulation of Caspase 3, Caspase 8 and Caspase 9 expressions (P < 0.05). These results may be ascribed to the improvement in ROS levels in the fish gill (P < 0.05). Taken together, the results showed that valine deficiency impaired the structural integrity of fish gill by disrupted fish antioxidant defenses and regulating the expression of tight junction protein, cytokines, antioxidant enzymes, NF-kappa B p65, I kappa B alpha, TOR, Nrf2, Keap1 and apoptosis-related genes in the fish gill. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: NF-KAPPA-B ; MUCOSAL IMMUNE-SYSTEM ; SALMON SALMO-SALAR ; TROUT ONCORHYNCHUS-MYKISS ; FATTY-ACID-COMPOSITION ; CHAIN AMINO-ACIDS ; OXIDATIVE STRESS ; CYPRINUS-CARPIO ; STENOHALINE GOLDFISH ; AQUATIC ORGANISMS
语种: 英语
WOS记录号: WOS:000358626800005
ISSN号: 1050-4648
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25963
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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