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题名: Highly efficient microfluidic sorting device for synchronizing developmental stages of C. elegans based on deflecting electrotaxis
作者: Wang, Xixian1, 2; Hu, Rui1, 2, 3; Ge, Anle1, 2; Hu, Liang1, 2; Wang, Shanshan1, 2; Feng, Xiaojun1, 2; Du, Wei1, 2; Liu, Bi-Feng1, 2
刊名: LAB ON A CHIP
发表日期: 2015
DOI: 10.1039/c5lc00354g
卷: 15, 期:11, 页:2513-2521
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
英文摘要: C. elegans as a powerful model organism has been widely used in fundamental biological studies. Many of these studies frequently need a large number of different stage-synchronized worms due to the stage-specific features of C. elegans among 4 distinct larval stages and the adult stage. In this work, we present an interesting and cost-effective microfluidic approach to realize simultaneous sorting of C. elegans of different developmental stages by deflecting electrotaxis. The microfluidic device was fabricated using PDMS consisting of symmetric sorting channels with specific angles, which was further hybridized to an agarose plate. While applying an electric field, different stages of C. elegans would crawl to the negative pore with different angles due to their deflecting electrotaxis. Thus, the worms were separated and synchronized by stages. lon-2 mutant was further used to study this electrotactic response and the results indicated that the body size plays a key role in determining the deflecting angle in matured adult worms. In addition to discriminating wild-type hermaphrodites, it could also be employed to sort mutants with abnormal development sizes and males. Therefore, our device provided a versatile and highly efficient platform for sorting C. elegans to meet the requirement of large numbers of different stage-synchronized worms. It can also be further used to investigate the neuronal basis of deflecting electrotaxis in worms.
关键词[WOS]: NEMATODE CAENORHABDITIS-ELEGANS ; IN-VIVO ; NEURONAL-ACTIVITY ; CHIP ; SYSTEM ; BEHAVIOR ; PLATFORM ; SCREENS
语种: 英语
WOS记录号: WOS:000354963400020
ISSN号: 1473-0197
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25891
Appears in Collections:其他_期刊论文

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作者单位: 1.Huazhong Univ Sci & Technol, Britton Chance Ctr Biomed Photon, Wuhan Natl Lab Optoelect Hubei Bioinformat, Coll Life Sci & Technol, Wuhan 430074, Peoples R China
2.Huazhong Univ Sci & Technol, Mol Imaging Key Lab, Dept Biomed Engn, Syst Biol Theme,Coll Life Sci & Technol, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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