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Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda
Gao, Zhi Peng1,2,3; Nie, Pin1; Lu, Jin Fang1,3; Liu, Lu Yi1; Xiao, Tiao Yi2; Liu, Wei1; Liu, Jia Shou1; Xie, Hai Xia1
2015-09-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume81Issue:17Pages:6078-6087
AbstractThe type III secretion system (T3SS) of Edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. EseB, a component required for effector translocation, is reported to mediate autoaggregation of E. tarda. In this study, we demonstrate that EseB forms filamentous appendages on the surface of E. tarda and is required for biofilm formation by E. tarda in Dulbecco's modified Eagle's medium (DMEM). Biofilm formation by E. tarda in DMEM does not require FlhB, an essential component for assembling flagella. Dynamic analysis of EseB filament formation, autoaggregation, and biofilm formation shows that the formation of EseB filaments occurs prior to autoaggregation and biofilm formation. The addition of an EseB antibody to E. tarda cultures before bacterial autoaggregation prevents autoaggregation and biofilm formation in a dose-dependent manner, whereas the addition of the EseB antibody to E. tarda cultures in which biofilm is already formed does not destroy the biofilm. Therefore, EseB filament-mediated bacterial cell-cell interaction is a prerequisite for autoaggregation and biofilm formation.
SubtypeArticle
DOI10.1128/AEM.01254-15
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS KeywordENTEROPATHOGENIC ESCHERICHIA-COLI ; PROTEIN TRANSLOCATION ; 2-COMPONENT SYSTEM ; EPITHELIAL-CELLS ; ESPA FILAMENTS ; VIRULENCE ; MOTILITY ; GENES ; MECHANISMS ; FLAGELLA
WOS IDWOS:000359259000043
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25888
Collection鱼类生物学及渔业生物技术研究中心
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China
2.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Gao, Zhi Peng,Nie, Pin,Lu, Jin Fang,et al. Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2015,81(17):6078-6087.
APA Gao, Zhi Peng.,Nie, Pin.,Lu, Jin Fang.,Liu, Lu Yi.,Xiao, Tiao Yi.,...&Xie, Hai Xia.(2015).Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,81(17),6078-6087.
MLA Gao, Zhi Peng,et al."Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 81.17(2015):6078-6087.
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