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题名: Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda
作者: Gao, Zhi Peng1, 2, 3; Nie, Pin1; Lu, Jin Fang1, 3; Liu, Lu Yi1; Xiao, Tiao Yi2; Liu, Wei1; Liu, Jia Shou1; Xie, Hai Xia1
刊名: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
发表日期: 2015-09-01
DOI: 10.1128/AEM.01254-15
卷: 81, 期:17, 页:6078-6087
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
英文摘要: The type III secretion system (T3SS) of Edwardsiella tarda plays an important role in infection by translocating effector proteins into host cells. EseB, a component required for effector translocation, is reported to mediate autoaggregation of E. tarda. In this study, we demonstrate that EseB forms filamentous appendages on the surface of E. tarda and is required for biofilm formation by E. tarda in Dulbecco's modified Eagle's medium (DMEM). Biofilm formation by E. tarda in DMEM does not require FlhB, an essential component for assembling flagella. Dynamic analysis of EseB filament formation, autoaggregation, and biofilm formation shows that the formation of EseB filaments occurs prior to autoaggregation and biofilm formation. The addition of an EseB antibody to E. tarda cultures before bacterial autoaggregation prevents autoaggregation and biofilm formation in a dose-dependent manner, whereas the addition of the EseB antibody to E. tarda cultures in which biofilm is already formed does not destroy the biofilm. Therefore, EseB filament-mediated bacterial cell-cell interaction is a prerequisite for autoaggregation and biofilm formation.
关键词[WOS]: ENTEROPATHOGENIC ESCHERICHIA-COLI ; PROTEIN TRANSLOCATION ; 2-COMPONENT SYSTEM ; EPITHELIAL-CELLS ; ESPA FILAMENTS ; VIRULENCE ; MOTILITY ; GENES ; MECHANISMS ; FLAGELLA
语种: 英语
WOS记录号: WOS:000359259000043
ISSN号: 0099-2240
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25888
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China
2.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
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