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Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins
Zhang, De Lu1; Zhang, Jing2; Hu, Chun Xiang3; Wang, Gao Hong3; Li, Dun Hai3; Liu, Yong Ding3; Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2014-12-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume157Pages:215-224
AbstractAphanizomenon flos-aquae is a cyanobacterium that produces neurotoxins or paralytic shellfish poisons (PSPs) called aphantoxins, which present threats to environmental safety and human health via eutrophication of water bodies worldwide. Although the molecular mechanisms of this neurotoxin have been studied, many questions remain unsolved, including those relating to in vivo hepatic neurotransmitter inactivation, physiological detoxification and histological and ultrastructural alterations. Aphantoxins extracted from the natural strain of A. flos-aquae DC-1 were analyzed by high-performance liquid chromatography. The main components were gonyautoxins 1 and 5 (GTX1, GTX5) and neosaxitoxin (neoSTX), which comprised 34.04%, 21.28%, and 12.77% respectively. Zebrafish (Danio rerio) were exposed intraperitoneally to 5.3 or 7.61 mu g STX equivalents (eq)/kg (low and high doses, respectively) of A. flos-aquae DC-1 aphantoxins. Morphological alterations and changes in neurotransmitter conduction functions of acetylcholinesterase (AChE) and monoamine oxidase (MAO) in zebrafish liver were detected at different time points 1-24 h post-exposure. Aphantoxin significantly enhanced hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and histological and ultrastructural damage in zebrafish liver at 3-12 h post-exposure. Toxin exposure increased the reactive oxygen species content and reduced total antioxidative capacity in zebrafish liver, suggesting oxidative stress. AChE and MAO activities were significantly inhibited, suggesting neurotransmitter inactivation/conduction function abnormalities in zebrafish liver. All alterations were dose- and time-dependent. Overall, the results indicate that aphantoxins/PSPs induce oxidative stress through inhibition of AChE and MAO activities, leading to neurotoxicity in zebrafish liver. The above parameters may be useful as bioindicators for investigating aphantoxins/PSPs and cyanobacterial blooms in nature. (C) 2014 Published by Elsevier B.V.
SubtypeArticle
KeywordCyanobacterial Neurotoxin Zebrafish Liver Morphological Alteration Acetylcholinesterase Monoamine Oxidase Neurotoxicity
Department[Zhang, De Lu] Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China ; [Zhang, Jing] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China ; [Hu, Chun Xiang ; Wang, Gao Hong ; Li, Dun Hai ; Liu, Yong Ding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
DOI10.1016/j.aquatox.2014.10.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000346547800023
WOS KeywordSHELLFISH POISONING TOXINS ; DICENTRARCHUS-LABRAX L. ; CARP CYPRINUS-CARPIO ; CARASSIUS-AURATUS ; BIOCHEMICAL PARAMETERS ; CYANOBACTERIAL BLOOM ; LIPID-PEROXIDATION ; OXIDATIVE STRESS ; GRUZA RESERVOIR ; SAUDI-ARABIA
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25782
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China
2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, De Lu,Zhang, Jing,Hu, Chun Xiang,et al. Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins[J]. AQUATIC TOXICOLOGY,2014,157:215-224.
APA Zhang, De Lu.,Zhang, Jing.,Hu, Chun Xiang.,Wang, Gao Hong.,Li, Dun Hai.,...&Hu, CX .(2014).Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins.AQUATIC TOXICOLOGY,157,215-224.
MLA Zhang, De Lu,et al."Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins".AQUATIC TOXICOLOGY 157(2014):215-224.
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