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题名: Morphological alterations and acetylcholinesterase and monoamine oxidase inhibition in liver of zebrafish exposed to Aphanizomenon flos-aquae DC-1 aphantoxins
作者: Zhang, De Lu1; Zhang, Jing2; Hu, Chun Xiang3; Wang, Gao Hong3; Li, Dun Hai3; Liu, Yong Ding3
通讯作者: Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Cyanobacterial neurotoxin ; Zebrafish liver ; Morphological alteration ; Acetylcholinesterase ; Monoamine oxidase ; Neurotoxicity
刊名: AQUATIC TOXICOLOGY
发表日期: 2014-12-01
DOI: 10.1016/j.aquatox.2014.10.016
卷: 157, 页:215-224
收录类别: SCI
文章类型: Article
部门归属: [Zhang, De Lu] Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China ; [Zhang, Jing] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China ; [Hu, Chun Xiang ; Wang, Gao Hong ; Li, Dun Hai ; Liu, Yong Ding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
英文摘要: Aphanizomenon flos-aquae is a cyanobacterium that produces neurotoxins or paralytic shellfish poisons (PSPs) called aphantoxins, which present threats to environmental safety and human health via eutrophication of water bodies worldwide. Although the molecular mechanisms of this neurotoxin have been studied, many questions remain unsolved, including those relating to in vivo hepatic neurotransmitter inactivation, physiological detoxification and histological and ultrastructural alterations. Aphantoxins extracted from the natural strain of A. flos-aquae DC-1 were analyzed by high-performance liquid chromatography. The main components were gonyautoxins 1 and 5 (GTX1, GTX5) and neosaxitoxin (neoSTX), which comprised 34.04%, 21.28%, and 12.77% respectively. Zebrafish (Danio rerio) were exposed intraperitoneally to 5.3 or 7.61 mu g STX equivalents (eq)/kg (low and high doses, respectively) of A. flos-aquae DC-1 aphantoxins. Morphological alterations and changes in neurotransmitter conduction functions of acetylcholinesterase (AChE) and monoamine oxidase (MAO) in zebrafish liver were detected at different time points 1-24 h post-exposure. Aphantoxin significantly enhanced hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and histological and ultrastructural damage in zebrafish liver at 3-12 h post-exposure. Toxin exposure increased the reactive oxygen species content and reduced total antioxidative capacity in zebrafish liver, suggesting oxidative stress. AChE and MAO activities were significantly inhibited, suggesting neurotransmitter inactivation/conduction function abnormalities in zebrafish liver. All alterations were dose- and time-dependent. Overall, the results indicate that aphantoxins/PSPs induce oxidative stress through inhibition of AChE and MAO activities, leading to neurotoxicity in zebrafish liver. The above parameters may be useful as bioindicators for investigating aphantoxins/PSPs and cyanobacterial blooms in nature. (C) 2014 Published by Elsevier B.V.
关键词[WOS]: SHELLFISH POISONING TOXINS ; DICENTRARCHUS-LABRAX L. ; CARP CYPRINUS-CARPIO ; CARASSIUS-AURATUS ; BIOCHEMICAL PARAMETERS ; CYANOBACTERIAL BLOOM ; LIPID-PEROXIDATION ; OXIDATIVE STRESS ; GRUZA RESERVOIR ; SAUDI-ARABIA
语种: 英语
WOS记录号: WOS:000346547800023
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25782
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China
2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
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