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题名: Metabolic engineering of microorganisms to produce omega-3 very long-chain polyunsaturated fatty acids
作者: Gong, Yangmin1; Wan, Xia1; Jiang, Mulan1; Hu, Chuanjiong1; Hu, Hanhua2; Huang, Fenghong3
通讯作者: Gong, YM (reprint author), Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, 2 Xudong Second Rd, Wuhan 430062, Peoples R China.
关键词: Desaturases ; Elongases ; Metabolic engineering ; Microorganisms ; Omega-3 fatty acids ; Polyketide synthase-like pathway
刊名: PROGRESS IN LIPID RESEARCH
发表日期: 2014-10-01
DOI: 10.1016/j.plipres.2014.07.001
卷: 56, 页:19-35
收录类别: SCI
文章类型: Review
部门归属: [Gong, Yangmin ; Wan, Xia ; Jiang, Mulan ; Hu, Chuanjiong] Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China ; [Hu, Hanhua] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Huang, Fenghong] Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr, Wuhan 430062, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Nutrition & Dietetics
研究领域[WOS]: Biochemistry & Molecular Biology ; Nutrition & Dietetics
英文摘要: Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) have received growing attention due to their significant roles in human health. Currently the main source of these nutritionally and medically important fatty acids is marine fish, which has not met ever-increasing global demand. Microorganisms are an important alternative source also being explored. Although many microorganisms accumulate omega-3 LC-PUFAs naturally, metabolic engineering might still be necessary for significantly improving their yields. Here, we review recent research involving the engineering of microorganisms for production of omega-3 LC-PUFAs, including eicospentaenoic acid and docosohexaenoic acid. Both reconstitution of omega-3 LC-PUFA biosynthetic pathways and modification of existing pathways in microorganisms have demonstrated the potential to produce high levels of omega-3 LC-PUFAs. However, the yields of omega-3 LC-PUFAs in host systems have been substantially limited by potential metabolic bottlenecks, which might be caused partly by inefficient flux of fatty acid intermediates between the acyl-CoA and different lipid class pools. Although fatty acid flux in both native and heterologous microbial hosts might be controlled by several acyltransferases, evidence has suggested that genetic manipulation of one acyltransferase alone could significantly increase the accumulation of LC-PUFAs. The number of oleaginous microorganisms that can be genetically transformed is increasing, which will advance engineering efforts to maximize LC-PUFA yields in microbial strains. (C) 2014 Elsevier Ltd. All rights reserved.
关键词[WOS]: DIATOM PHAEODACTYLUM-TRICORNUTUM ; TYPE-2 DIACYLGLYCEROL ACYLTRANSFERASE ; YEAST RHODOSPORIDIUM-TORULOIDES ; BIOSYNTHESIS GENE-CLUSTER ; MORTIERELLA-ALPINA 1S-4 ; CORONARY HEART-DISEASE ; COENZYME-A SYNTHETASE ; EICOSAPENTAENOIC ACID ; DOCOSAHEXAENOIC-ACID ; ESCHERICHIA-COLI
语种: 英语
WOS记录号: WOS:000347129200002
ISSN号: 0163-7827
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25781
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr, Wuhan 430062, Peoples R China
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