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Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins
Zhang, De Lu1; Liu, Si Yi1; Zhang, Jing2; Hu, Chun Xiang3; Li, Dun Hai3; Liu, Yong Ding3; Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2015-03-01
Source PublicationECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN0147-6513
Volume113Pages:425-432
AbstractAphanizomenon flos-aquae secretes paralytic shellfish poisons (PSPs), termed aphantoxins, and endangers environmental and human health via eutrophication of water worldwide. Although the molecular mechanism of neuronal PSP toxicity has been well studied, several issues remain unresolved, notably the in vivo hepatic antioxidative responses to this neurotoxin. Aphantoxins extracted from a natural isolate of A. flos-aquae DC-1 were resolved by high performance liquid chromatography. The primary components were gonyautoxins 1 and 5 and neosaxitoxin. Zebrafish (Danio rerio) were treated intraperitoneally with either 53 or 7.61 (low and high doses, respectively) mu g saxitoxin (STX) equivalents (eq)/kg of A. flos-aquae DC-1 aphantoxins. Antioxidative responses in zebrafish liver were examined at different timepoints 1-24 h post-exposure. Aphantoxin administration significantly enhanced hepatic malondialdehyde (MDA) content 1-12 h post-exposure, indicative of oxidative stress and lipid peroxidation. By contrast, levels of reduced glutathione (GSH) in zebrafish liver declined significantly after 3-24 h exposure, suggesting that GSH participates in MDA metabolism. A significant upregulation of the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) was observed, suggesting that aphantoxins induce lipid peroxidation in zebrafish liver and are likely to be hepatotoxic. Hepatic levels of MDA and GSH, and of the three enzymes (SOD, CAT, and GPx), therefore provide potential biomarkers for studying environmental exposure to aphantoxins/PSPs from cyanobacterial blooms. (C) 2014 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordCyanobacterial Neurotoxins Zebrafish Liver Antioxidative Response Biomarkers Hepatotoxicity
Department[Zhang, De Lu ; Liu, Si Yi] Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China ; [Zhang, Jing] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China ; [Hu, Chun Xiang ; Li, Dun Hai ; Liu, Yong Ding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
DOI10.1016/j.ecoenv.2014.12.029
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS KeywordTILAPIA OREOCHROMIS-NILOTICUS ; SUPEROXIDE-DISMUTASE SOD ; OXIDATIVE STRESS ; MICROCYSTIN-LR ; LIPID-PEROXIDATION ; GLUTATHIONE-PEROXIDASE ; LABORATORY CONDITIONS ; CYANOBACTERIAL BLOOM ; TOXIC DINOFLAGELLATE ; AGGRESSIVE-BEHAVIOR
WOS IDWOS:000348966400050
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25774
Collection藻类生物学及应用研究中心
Corresponding AuthorHu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China
2.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, De Lu,Liu, Si Yi,Zhang, Jing,et al. Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2015,113:425-432.
APA Zhang, De Lu.,Liu, Si Yi.,Zhang, Jing.,Hu, Chun Xiang.,Li, Dun Hai.,...&Hu, CX .(2015).Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,113,425-432.
MLA Zhang, De Lu,et al."Antioxidative responses in zebrafish liver exposed to sublethal doses Aphanizomenon flos-aquae DC-1 aphantoxins".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 113(2015):425-432.
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