IHB OpenIR  > 藻类生物学及应用研究中心
The HetR-binding site that activates expression of patA in vegetative cells is required for normal heterocyst patterning in Anabaena sp PCC 7120
Hou, Shengwei; Zhou, Fang; Peng, Shan; Gao, Hong; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2015
Source PublicationSCIENCE BULLETIN
ISSN2095-9273
Volume60Issue:2Pages:192-201
AbstractUpon nitrogen step-down, Anabaena sp. PCC 7120 produces semi-regularly spaced heterocysts along filaments. HetR, the master regulator of heterocyst differentiation, has been shown to up-regulate hetP and hetZ in differentiating heterocysts via specific recognition sites. HetR is also predicted to bind to the promoter of patA, a gene required for heterocyst formation at intercalary positions. Here, we show that HetR binds to the predicted site 5' from patA. Moreover, (1) deletion of the HetR-binding site greatly diminished the expression of patA in vegetative cells as shown with gfp, and (2) complementation of a patA mutant by a replicating plasmid that bears patA is largely prevented by removal of that binding site. In contrast, HetR-binding sites suppressed the expression of alr0202 (a homolog of hetZ) specifically in heterocysts and of alr3234 (a homolog of hetP) in whole filaments. Our results indicate that HetR can regulate gene expression in different modes.
SubtypeArticle
KeywordAnabaena Heterocyst Hetr-binding Site Pata
Department[Hou, Shengwei ; Zhou, Fang ; Peng, Shan ; Gao, Hong ; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1007/s11434-014-0724-5
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordSP STRAIN PCC-7120 ; ESCHERICHIA-COLI ; DIFFERENTIATION ; GENE ; PROTEINS ; PCC7120 ; TARGETS
WOS IDWOS:000348927000004
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25773
Collection藻类生物学及应用研究中心
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Hou, Shengwei,Zhou, Fang,Peng, Shan,et al. The HetR-binding site that activates expression of patA in vegetative cells is required for normal heterocyst patterning in Anabaena sp PCC 7120[J]. SCIENCE BULLETIN,2015,60(2):192-201.
APA Hou, Shengwei,Zhou, Fang,Peng, Shan,Gao, Hong,Xu, Xudong,&Xu, XD .(2015).The HetR-binding site that activates expression of patA in vegetative cells is required for normal heterocyst patterning in Anabaena sp PCC 7120.SCIENCE BULLETIN,60(2),192-201.
MLA Hou, Shengwei,et al."The HetR-binding site that activates expression of patA in vegetative cells is required for normal heterocyst patterning in Anabaena sp PCC 7120".SCIENCE BULLETIN 60.2(2015):192-201.
Files in This Item:
File Name/Size DocType Version Access License
The HetR-binding sit(1100KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hou, Shengwei]'s Articles
[Zhou, Fang]'s Articles
[Peng, Shan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hou, Shengwei]'s Articles
[Zhou, Fang]'s Articles
[Peng, Shan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hou, Shengwei]'s Articles
[Zhou, Fang]'s Articles
[Peng, Shan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: The HetR-binding site that activates expression of patA in vegetative cells is required for normal heterocyst patterning in Anabaena sp PCC 7120.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.