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题名: A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803
作者: Daddy, Soumana3; Zhan, Jiao1, 2; Jantaro, Saowarath5; He, Chenliu1; He, Qingfang3, 4; Wang, Qiang1
通讯作者: He, QF (reprint author), Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA.
刊名: SCIENTIFIC REPORTS
发表日期: 2015-03-30
DOI: 10.1038/srep09480
卷: 5
收录类别: SCI
文章类型: Article
部门归属: [Zhan, Jiao ; He, Chenliu ; Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Zhan, Jiao] Univ Chinese Acad Sci, Beijing 100039, Peoples R China ; [Daddy, Soumana ; He, Qingfang] Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA ; [He, Qingfang] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China ; [Jantaro, Saowarath] Chulalongkorn Univ, Fac Sci, Dept Biochem, Bangkok 10330, Thailand
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Synechocystis sp. PCC 6803 is a model cyanobacterium extensively used to study photosynthesis. Here we reveal a novel high light-inducible carotenoid-binding protein complex (HLCC) in the thylakoid membranes of Synechocystis PCC 6803 cells exposed to high intensity light. Zeaxanthin and myxoxanthophyll accounted for 29.8% and 54.8%, respectively, of the carotenoids bound to the complex. Using Blue-Native PAGE followed by 2D SDS-PAGE and mass spectrometry, we showed that the HLCC consisted of Slr1128, IsiA, PsaD, and HliA/B. We confirmed these findings by SEAD fluorescence cross-linking and anti-PsaD immuno-coprecipitation analyses. The expression of genes encoding the protein components of the HLCC was enhanced by high light illumination and artificial oxidative stress. Deletion of these proteins resulted in impaired state transition and increased sensitivity to oxidative and/or high light stress, as indicated by increased membrane peroxidation. Therefore, the HLCC protects thylakoid membranes from extensive photooxidative damage, likely via a mechanism involving state transition.
关键词[WOS]: PHOTOSYSTEM-I COMPLEX ; CYANOBACTERIUM SPIRULINA-PLATENSIS ; SP PCC-6803 ; FLUORESCENCE SPECTROSCOPY ; ANTENNA CHLOROPHYLLS ; STATISTICAL-MODEL ; ENERGY-TRANSFER ; CORE COMPLEXES ; HIGHER-PLANTS ; SUBUNIT
语种: 英语
WOS记录号: WOS:000351939500002
ISSN号: 2045-2322
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/25765
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA
4.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
5.Chulalongkorn Univ, Fac Sci, Dept Biochem, Bangkok 10330, Thailand
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