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A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803
Daddy, Soumana3; Zhan, Jiao1,2; Jantaro, Saowarath5; He, Chenliu1; He, Qingfang3,4; Wang, Qiang1; He, QF (reprint author), Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA.
2015-03-30
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume5
AbstractSynechocystis sp. PCC 6803 is a model cyanobacterium extensively used to study photosynthesis. Here we reveal a novel high light-inducible carotenoid-binding protein complex (HLCC) in the thylakoid membranes of Synechocystis PCC 6803 cells exposed to high intensity light. Zeaxanthin and myxoxanthophyll accounted for 29.8% and 54.8%, respectively, of the carotenoids bound to the complex. Using Blue-Native PAGE followed by 2D SDS-PAGE and mass spectrometry, we showed that the HLCC consisted of Slr1128, IsiA, PsaD, and HliA/B. We confirmed these findings by SEAD fluorescence cross-linking and anti-PsaD immuno-coprecipitation analyses. The expression of genes encoding the protein components of the HLCC was enhanced by high light illumination and artificial oxidative stress. Deletion of these proteins resulted in impaired state transition and increased sensitivity to oxidative and/or high light stress, as indicated by increased membrane peroxidation. Therefore, the HLCC protects thylakoid membranes from extensive photooxidative damage, likely via a mechanism involving state transition.
SubtypeArticle
Department[Zhan, Jiao ; He, Chenliu ; Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Zhan, Jiao] Univ Chinese Acad Sci, Beijing 100039, Peoples R China ; [Daddy, Soumana ; He, Qingfang] Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA ; [He, Qingfang] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China ; [Jantaro, Saowarath] Chulalongkorn Univ, Fac Sci, Dept Biochem, Bangkok 10330, Thailand
DOI10.1038/srep09480
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordPHOTOSYSTEM-I COMPLEX ; CYANOBACTERIUM SPIRULINA-PLATENSIS ; SP PCC-6803 ; FLUORESCENCE SPECTROSCOPY ; ANTENNA CHLOROPHYLLS ; STATISTICAL-MODEL ; ENERGY-TRANSFER ; CORE COMPLEXES ; HIGHER-PLANTS ; SUBUNIT
WOS IDWOS:000351939500002
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25765
Collection藻类生物学及应用研究中心
Corresponding AuthorHe, QF (reprint author), Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Univ Arkansas, Dept Biol, Little Rock, AR 72204 USA
4.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China
5.Chulalongkorn Univ, Fac Sci, Dept Biochem, Bangkok 10330, Thailand
Recommended Citation
GB/T 7714
Daddy, Soumana,Zhan, Jiao,Jantaro, Saowarath,et al. A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803[J]. SCIENTIFIC REPORTS,2015,5.
APA Daddy, Soumana.,Zhan, Jiao.,Jantaro, Saowarath.,He, Chenliu.,He, Qingfang.,...&He, QF .(2015).A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803.SCIENTIFIC REPORTS,5.
MLA Daddy, Soumana,et al."A novel high light-inducible carotenoid-binding protein complex in the thylakoid membranes of Synechocystis PCC 6803".SCIENTIFIC REPORTS 5(2015).
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