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Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences
Zhu, Mengling1,2; Yu, Gongliang3; Song, Gaofei3; Chang, Jianbo1,2; Wan, Chengyan1,2; Li, Renhui3; Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
2015-06-01
Source PublicationBIOCHEMICAL SYSTEMATICS AND ECOLOGY
ISSN0305-1978
Volume60Pages:110-115
AbstractIn decades, Pseudanabaena species have been frequently reported as cyanobacterial bloom components. However, these species are always overlooked because they cannot be observed easily owing to their small sizes. This study aimed to characterize Pseudanabaena species and detect them in field waters by developing an effective DNA marker. A region with approximately 600 bp in the rbcLX sequences was specific to Pseudanabaena species alone compared with other cyanobacterial genera. Therefore, new primers specific for Pseudanabaena were generated. By using this molecular tool, seasonal and spatial dynamics of Pseudanabaena populations of six sites in Lushui Reservoir, China were measured from July 2010 to August 2011. Pseudanabaena species existed in the reservoir throughout the year, and cell abundance ranged between 10(4) and 10(6) equivalent cells/L, with the peak at 8.07 x 10(6) equivalent cells/L The seasonal dynamics pattern of Pseudanabaena was similar to that of Microcystis but showed a slight lag at the transitional points of water temperature in the reservoir. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordRbclx Cyanobacteria Molecular Detection Pseudanabaena Realtime-pcr
Department[Zhu, Mengling ; Chang, Jianbo ; Wan, Chengyan] Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan 430079, Peoples R China ; [Zhu, Mengling ; Chang, Jianbo ; Wan, Chengyan] Chinese Acad Sci, Wuhan 430079, Peoples R China ; [Yu, Gongliang ; Song, Gaofei ; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
DOI10.1016/j.bse.2015.04.009
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS KeywordSTRAINS ; QUANTIFICATION ; OSCILLATORIALES ; PHYTOPLANKTON ; SYSTEMS ; CHINA
WOS IDWOS:000357546800017
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/25752
Collection藻类生物学及应用研究中心
Corresponding AuthorLi, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
Affiliation1.Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan 430079, Peoples R China
2.Chinese Acad Sci, Wuhan 430079, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Mengling,Yu, Gongliang,Song, Gaofei,et al. Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2015,60:110-115.
APA Zhu, Mengling.,Yu, Gongliang.,Song, Gaofei.,Chang, Jianbo.,Wan, Chengyan.,...&Li, RH .(2015).Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,60,110-115.
MLA Zhu, Mengling,et al."Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 60(2015):110-115.
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