| Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences | |
| Zhu, Mengling1,2; Yu, Gongliang3; Song, Gaofei3; Chang, Jianbo1,2; Wan, Chengyan1,2; Li, Renhui3; Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China. | |
| 2015-06-01 | |
| Source Publication | BIOCHEMICAL SYSTEMATICS AND ECOLOGY
 |
| ISSN | 0305-1978 |
| Volume | 60Pages:110-115 |
| Abstract | In decades, Pseudanabaena species have been frequently reported as cyanobacterial bloom components. However, these species are always overlooked because they cannot be observed easily owing to their small sizes. This study aimed to characterize Pseudanabaena species and detect them in field waters by developing an effective DNA marker. A region with approximately 600 bp in the rbcLX sequences was specific to Pseudanabaena species alone compared with other cyanobacterial genera. Therefore, new primers specific for Pseudanabaena were generated. By using this molecular tool, seasonal and spatial dynamics of Pseudanabaena populations of six sites in Lushui Reservoir, China were measured from July 2010 to August 2011. Pseudanabaena species existed in the reservoir throughout the year, and cell abundance ranged between 10(4) and 10(6) equivalent cells/L, with the peak at 8.07 x 10(6) equivalent cells/L The seasonal dynamics pattern of Pseudanabaena was similar to that of Microcystis but showed a slight lag at the transitional points of water temperature in the reservoir. (C) 2015 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Rbclx Cyanobacteria Molecular Detection Pseudanabaena Realtime-pcr |
| Department | [Zhu, Mengling ; Chang, Jianbo ; Wan, Chengyan] Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan 430079, Peoples R China ; [Zhu, Mengling ; Chang, Jianbo ; Wan, Chengyan] Chinese Acad Sci, Wuhan 430079, Peoples R China ; [Yu, Gongliang ; Song, Gaofei ; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China |
| DOI | 10.1016/j.bse.2015.04.009 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| WOS Keyword | STRAINS ; QUANTIFICATION ; OSCILLATORIALES ; PHYTOPLANKTON ; SYSTEMS ; CHINA |
| WOS ID | WOS:000357546800017 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/25752 |
| Collection | 藻类生物学及应用研究中心 |
| Corresponding Author | Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Minist Water Resources, Inst Hydroecol, Key Lab Ecol Impacts Hydraul Projects & Restorat, Wuhan 430079, Peoples R China 2.Chinese Acad Sci, Wuhan 430079, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, Mengling,Yu, Gongliang,Song, Gaofei,et al. Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2015,60:110-115. |
| APA | Zhu, Mengling.,Yu, Gongliang.,Song, Gaofei.,Chang, Jianbo.,Wan, Chengyan.,...&Li, RH .(2015).Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,60,110-115. |
| MLA | Zhu, Mengling,et al."Molecular specificity and detection for Pseudanabaena (cyanobacteria) species based on rbcLX sequences".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 60(2015):110-115. |
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