中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides
作者: Lu, Xing1; Long, Yong2; Lin, Li1; Sun, Rongze1; Zhong, Shan1; Cui, Zongbin2
通讯作者: Zhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China.
刊名: PLOS ONE
发表日期: 2014-12-05
DOI: 10.1371/journal.pone.0111664
卷: 9, 期:12, 页:e111664
收录类别: SCI
文章类型: Article
部门归属: [Lu, Xing ; Lin, Li ; Sun, Rongze ; Zhong, Shan] Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China ; [Long, Yong ; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: DDT and lindane are highly toxic organochlorine pesticides and posing adverse effects on the environment and public health due to their frequent usage in developing countries. ABCC4/MRP4 is an organic anion transporter that mediates cellular efflux of a wide range of exogenous and endogenous compounds such as cyclic nucleotides and anti-cancer drugs; however, it remains unclear whether ABCC4 and its orthologs function in the detoxification of organochlorine pesticides. Here, we demonstrated the roles of zebrafish Abcc4 in cellular efflux of DDT and lindane. Zebrafish abcc4 was maternally expressed in the oocytes and its transcripts were detected in the lens, pancreas, gills, liver, intestine and bladder of developing embryos and in adult tissues examined. DDT and lindane were able to induce the expression of abcc4 gene and overexpression of Abcc4 significantly decreased the cytotoxicity and accumulation of DDT and lindane in LLC-PK1 cells and developing embryos. In contrast, overexpression of an Abcc4-G1188D mutant abolished its transporter function without effects on its substrate binding activity, and sensitized LLC-PK1 cells and developing embryos to toxic pesticides. Moreover, glutathione (GSH) was involved in the efflux of cellular pesticides and ATPase activity in developing embryos can be induced by DDT or lindane. Thus, zebrafish Abcc4 plays crucial roles in cellular efflux of organochlorine pesticides and can be used a potential molecular marker for the monitor of DDT and lindane contamination in the aquatic environment.
关键词[WOS]: MULTIDRUG-RESISTANCE PROTEINS ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; DIGESTIVE GLAND ; P-GLYCOPROTEIN ; GLUTATHIONE ; MRP ; EXPRESSION ; MRP4/ABCC4 ; LINDANE ; GENE
语种: 英语
WOS记录号: WOS:000346907200003
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23996
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides.pdf(5143KB)----开放获取View Download

作者单位: 1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Lu, Xing]'s Articles
[Long, Yong]'s Articles
[Lin, Li]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Lu, Xing]‘s Articles
[Long, Yong]‘s Articles
[Lin, Li]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace