IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides
Lu, Xing1; Long, Yong2; Lin, Li1; Sun, Rongze1; Zhong, Shan1; Cui, Zongbin2; Zhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China.
2014-12-05
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:12Pages:e111664
AbstractDDT and lindane are highly toxic organochlorine pesticides and posing adverse effects on the environment and public health due to their frequent usage in developing countries. ABCC4/MRP4 is an organic anion transporter that mediates cellular efflux of a wide range of exogenous and endogenous compounds such as cyclic nucleotides and anti-cancer drugs; however, it remains unclear whether ABCC4 and its orthologs function in the detoxification of organochlorine pesticides. Here, we demonstrated the roles of zebrafish Abcc4 in cellular efflux of DDT and lindane. Zebrafish abcc4 was maternally expressed in the oocytes and its transcripts were detected in the lens, pancreas, gills, liver, intestine and bladder of developing embryos and in adult tissues examined. DDT and lindane were able to induce the expression of abcc4 gene and overexpression of Abcc4 significantly decreased the cytotoxicity and accumulation of DDT and lindane in LLC-PK1 cells and developing embryos. In contrast, overexpression of an Abcc4-G1188D mutant abolished its transporter function without effects on its substrate binding activity, and sensitized LLC-PK1 cells and developing embryos to toxic pesticides. Moreover, glutathione (GSH) was involved in the efflux of cellular pesticides and ATPase activity in developing embryos can be induced by DDT or lindane. Thus, zebrafish Abcc4 plays crucial roles in cellular efflux of organochlorine pesticides and can be used a potential molecular marker for the monitor of DDT and lindane contamination in the aquatic environment.
SubtypeArticle
Department[Lu, Xing ; Lin, Li ; Sun, Rongze ; Zhong, Shan] Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China ; [Long, Yong ; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
DOI10.1371/journal.pone.0111664
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000346907200003
WOS KeywordMULTIDRUG-RESISTANCE PROTEINS ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; DIGESTIVE GLAND ; P-GLYCOPROTEIN ; GLUTATHIONE ; MRP ; EXPRESSION ; MRP4/ABCC4 ; LINDANE ; GENE
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23996
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Lu, Xing,Long, Yong,Lin, Li,et al. Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides[J]. PLOS ONE,2014,9(12):e111664.
APA Lu, Xing.,Long, Yong.,Lin, Li.,Sun, Rongze.,Zhong, Shan.,...&Zhong, S .(2014).Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides.PLOS ONE,9(12),e111664.
MLA Lu, Xing,et al."Characterization of Zebrafish Abcc4 as an Efflux Transporter of Organochlorine Pesticides".PLOS ONE 9.12(2014):e111664.
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