Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity
Deng, Jiaoyu5; Bi, Lijun1,2,9; Zhou, Lin6; Guo, Shu-juan3,4; Fleming, Joy1,2; Jiang, He-wei3,4; Zhou, Ying1,2; Gu, Jia5; Zhong, Qiu6; Wang, Zong-xiu3,4; Liu, Zhonghui1,2; Deng, Rui-ping3,4; Gao, Jing5; Chen, Tao6; Li, Wenjuan1,2; Wang, Jing-fang3,4; Wang, Xude5; Li, Haicheng6; Ge, Feng7; Zhu, Guofeng8; Zhang, Hai-nan3,4; Gu, Jing5; Wu, Fan-lin3,4; Zhang, Zhiping5; Wang, Dianbing1,2; Hang, Haiying1,2,9; Li, Yang3,4; Cheng, Li3,4; He, Xiang3,4; Tao, Sheng-ce3,4; Zhang, Xian-En1,2; Tao, SC (reprint author), Chinese Acad Sci, Inst Biophys, Key Lab Non Coding RNA, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China.
2014-12-24
Source PublicationCELL REPORTS
ISSN2211-1247
Volume9Issue:6Pages:2317-2329
AbstractPoor understanding of the basic biology of Mycobacterium tuberculosis (MTB), the etiological agent of tuberculosis, hampers development of much-needed drugs, vaccines, and diagnostic tests. Better experimental tools are needed to expedite investigations of this pathogen at the systems level. Here, we present a functional MTB proteome microarray covering most of the proteome and an ORFome library. We demonstrate the broad applicability of the microarray by investigating global protein-protein interactions, small-molecule-proteinbinding, and serum biomarker discovery, identifying 59 PknG-interacting proteins, 30 bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) binding proteins, and 14 MTB proteins that together differentiate between tuberculosis (TB) patients with active disease and recovered individuals. Results suggest that the MTB rhamnose pathway is likely regulated by both the serine/threonine kinase PknG and c-di-GMP. This resource has the potential to generate a greater understanding of key biological processes in the pathogenesis of tuberculosis, possibly leading to more effective therapies for the treatment of this ancient disease.
SubtypeArticle
Department[Bi, Lijun ; Fleming, Joy ; Zhou, Ying ; Liu, Zhonghui ; Li, Wenjuan ; Wang, Dianbing ; Hang, Haiying ; Zhang, Xian-En] Chinese Acad Sci, Inst Biophys, Key Lab Non Coding RNA, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China ; [Bi, Lijun ; Fleming, Joy ; Zhou, Ying ; Liu, Zhonghui ; Li, Wenjuan ; Wang, Dianbing ; Hang, Haiying ; Zhang, Xian-En] Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China ; [Guo, Shu-juan ; Jiang, He-wei ; Wang, Zong-xiu ; Deng, Rui-ping ; Wang, Jing-fang ; Zhang, Hai-nan ; Wu, Fan-lin ; Li, Yang ; Cheng, Li ; He, Xiang ; Tao, Sheng-ce] Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Minist Educ, Shanghai Ctr Syst Biomed,Key Lab Syst Biomed, Shanghai 200240, Peoples R China ; [Guo, Shu-juan ; Jiang, He-wei ; Wang, Zong-xiu ; Deng, Rui-ping ; Wang, Jing-fang ; Zhang, Hai-nan ; Wu, Fan-lin ; Li, Yang ; Cheng, Li ; He, Xiang ; Tao, Sheng-ce] Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China ; [Deng, Jiaoyu ; Gu, Jia ; Gao, Jing ; Wang, Xude ; Gu, Jing ; Zhang, Zhiping] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China ; [Zhou, Lin ; Zhong, Qiu ; Chen, Tao ; Li, Haicheng] Ctr TB Control Guangdong Prov, Guangzhou 510630, Guangdong, Peoples R China ; [Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430071, Peoples R China ; [Zhu, Guofeng] Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China ; [Bi, Lijun ; Hang, Haiying] TB Healthcare Biotechnol Co Ltd, Foshan 528000, Guangdong, Peoples R China
DOI10.1016/j.celrep.2014.11.023
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS KeywordCYCLIC DI-GMP ; CELL-WALL ; KINASE-G ; REVEALS ; ASSAYS ; GENES ; PHOSPHORYLATION ; NORMALIZATION ; METABOLISM ; INHIBITION
WOS IDWOS:000346852400028
Citation statistics
Cited Times:41[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23995
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorTao, SC (reprint author), Chinese Acad Sci, Inst Biophys, Key Lab Non Coding RNA, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Biophys, Key Lab Non Coding RNA, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China
3.Shanghai Jiao Tong Univ, State Key Lab Oncogenes & Related Genes, Minist Educ, Shanghai Ctr Syst Biomed,Key Lab Syst Biomed, Shanghai 200240, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China
5.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
6.Ctr TB Control Guangdong Prov, Guangzhou 510630, Guangdong, Peoples R China
7.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430071, Peoples R China
8.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200336, Peoples R China
9.TB Healthcare Biotechnol Co Ltd, Foshan 528000, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Deng, Jiaoyu,Bi, Lijun,Zhou, Lin,et al. Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity[J]. CELL REPORTS,2014,9(6):2317-2329.
APA Deng, Jiaoyu.,Bi, Lijun.,Zhou, Lin.,Guo, Shu-juan.,Fleming, Joy.,...&Tao, SC .(2014).Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity.CELL REPORTS,9(6),2317-2329.
MLA Deng, Jiaoyu,et al."Mycobacterium Tuberculosis Proteome Microarray for Global Studies of Protein Function and Immunogenicity".CELL REPORTS 9.6(2014):2317-2329.
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