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Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)
Zhao, Juan1; Feng, Lin1,2,3; Liu, Yang1,2,3; Jiang, Weidan1,2,3; Wu, Pei1; Jiang, Jun1,2,3; Zhang, Yongan4; Zhou, Xiaoqiu1,2,3; Zhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China.
2014-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume41Issue:2Pages:663-673
AbstractThis study was conducted to investigate the effects of dietary isoleucine (Ile) on the immune response, antioxidant status, tight junctions, and microbial population in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 1200 juvenile jian carp with average initial weight 6.9 +/- 0.03 g were fed semi-purified isonitrogenous diets containing 4.2 (unsupplemented control group), 7.0, 9.5, 11.9, 13.9 and 16.9 g Ile kg(-1) diet for 60 days. Results indicated that Ile supplementation decreased malondialdehyde (MDA) and protein carbonyl content, and the amounts of Escherichia coli and Aeromonas in the intestine (P < 0.05), and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR), glutathione content and the amounts of Lactobacillus and Bacillus in the intestine (P < 0.05). Furthermore, real time polymerase chain reaction revealed that relative mRNA expression of copper/zinc superoxide dismutase (Cu-ZnSOD), manganese superoxide dismutase (MnSOD), CAT, NF-E2-related factor 2 (Nrf2), p38 mitogen-activated protein kinases (p38MAPK) in the intestine were increased with increasing of dietary Ile up to a certain point (P < 0.05). Conversely, the relative mRNA expression of occludin, claudin-3, daudin-7, TNF-alpha, IL-10, Kelch-like-ECH- associated protein 1 (Keap1), extracellular signal-regulated kinase 1 (ERK1) in the intestine showed a downward trend (P < 0.05). In conclusion, dietary Ile improves intestinal immune function, antioxidant capacity and microbial population, and regulates gene expression of antioxidant enzyme, tight junctions, Nrf2, Keap1, p38 and ERK1 in the intestine of Jian carp. (C) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordCyprinus Carpio Var. Jian Isoleucine Tight Junction Cytokine Nuclear Factor Erythroid 2 (Nf-e2)/p45-related Factor 2
Department[Zhao, Juan ; Feng, Lin ; Liu, Yang ; Jiang, Weidan ; Wu, Pei ; Jiang, Jun ; Zhou, Xiaoqiu] Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China ; [Feng, Lin ; Liu, Yang ; Jiang, Weidan ; Jiang, Jun ; Zhou, Xiaoqiu] Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Yaan 625014, Sichuan, Peoples R China ; [Feng, Lin ; Liu, Yang ; Jiang, Weidan ; Jiang, Jun ; Zhou, Xiaoqiu] Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr China, Yaan 625014, Sichuan, Peoples R China ; [Zhang, Yongan] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1016/j.fsi.2014.10.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000347017500065
WOS KeywordFED GRADED-LEVELS ; AMINO-ACID SUPPLEMENTATION ; BODY-COMPOSITION ; ENZYME-ACTIVITIES ; DIGESTIVE CAPACITY ; BARRIER FUNCTION ; GENE-EXPRESSION ; GROWTH-PERFORMANCE ; CLAUDIN EXPRESSION ; PROTEIN EXPRESSION
Citation statistics
Cited Times:45[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23993
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China.
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Yaan 625014, Sichuan, Peoples R China
3.Sichuan Agr Univ, Minist Educ, Key Lab Anim Dis Resistance Nutr China, Yaan 625014, Sichuan, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Juan,Feng, Lin,Liu, Yang,et al. Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J]. FISH & SHELLFISH IMMUNOLOGY,2014,41(2):663-673.
APA Zhao, Juan.,Feng, Lin.,Liu, Yang.,Jiang, Weidan.,Wu, Pei.,...&Zhou, XQ .(2014).Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian).FISH & SHELLFISH IMMUNOLOGY,41(2),663-673.
MLA Zhao, Juan,et al."Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)".FISH & SHELLFISH IMMUNOLOGY 41.2(2014):663-673.
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