Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes
Yang, Ming-kun1; Yang, Yao-hua1; Chen, Zhuo1; Zhang, Jia1; Lin, Yan1; Wang, Yan1; Xiong, Qian1; Li, Tao1; Ge, Feng1; Bryant, Donald A.2; Zhao, Jin-dong1; Li, T (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2014-12-30
Source PublicationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN0027-8424
Volume111Issue:52Pages:E5633-E5642
AbstractWe describe an integrated workflow for proteogenomic analysis and global profiling of posttranslational modifications (PTMs) in prokaryotes and use the model cyanobacterium Synechococcus sp. PCC 7002 (hereafter Synechococcus 7002) as a test case. We found more than 20 different kinds of PTMs, and a holistic view of PTM events in this organism grown under different conditions was obtained without specific enrichment strategies. Among 3,186 predicted protein-coding genes, 2,938 gene products (> 92%) were identified. We also identified 118 previously unidentified proteins and corrected 38 predicted gene-coding regions in the Synechococcus 7002 genome. This systematic analysis not only provides comprehensive information on protein profiles and the diversity of PTMs in Synechococcus 7002 but also provides some insights into photosynthetic pathways in cyanobacteria. The entire proteogenomics pipeline is applicable to any sequenced prokaryotic organism, and we suggest that it should become a standard part of genome annotation projects.
SubtypeArticle
KeywordProteogenomics Post-translational Modifications Cyanobacteria Photosynthesis Synechococcus
Department[Yang, Ming-kun ; Yang, Yao-hua ; Chen, Zhuo ; Zhang, Jia ; Lin, Yan ; Wang, Yan ; Xiong, Qian ; Li, Tao ; Ge, Feng ; Zhao, Jin-dong] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Bryant, Donald A.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
DOI10.1073/pnas.1412722111
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordSTRAIN PCC 7002 ; MASS-SPECTROMETRY ; SIGNAL PEPTIDES ; PROTEOME ANALYSIS ; ANALYSIS REVEALS ; CYANOBACTERIA ; IDENTIFICATION ; TRANSCRIPTOME ; ANNOTATION ; GENOME
WOS IDWOS:000347444400008
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23992
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorLi, T (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
Recommended Citation
GB/T 7714
Yang, Ming-kun,Yang, Yao-hua,Chen, Zhuo,et al. Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2014,111(52):E5633-E5642.
APA Yang, Ming-kun.,Yang, Yao-hua.,Chen, Zhuo.,Zhang, Jia.,Lin, Yan.,...&Li, T .(2014).Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,111(52),E5633-E5642.
MLA Yang, Ming-kun,et al."Proteogenomic analysis and global discovery of posttranslational modifications in prokaryotes".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111.52(2014):E5633-E5642.
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