Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR
Lu, Xing1; Long, Yong2; Sun, Rongze1; Zhou, Bolan2; Lin, Li1; Zhong, Shan1; Cui, Zongbin2; Zhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China.
2015
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN1532-0456
Volume167Pages:35-42
AbstractMicrocystin-LR (MC-LR) is one of the most common microcystins (MCs), which are hepatotoxic and released into a water body during a period of cyanobacterial blooms. These toxicants can be accumulated in aquatic animals and transferred along the food chain and thus pose adverse effects on aquatic environment and public health. Zebrafish Abcb4 is reported to mediate the cellular efflux of ecotoxicologically relevant compounds including galaxolide, tonalide and phenanthrene; however, it remains unclear whether Abcb4 functions in the detoxification of MC-LR. Here, we demonstrated the role of zebrafish Abcb4 in cellular efflux of MC-LR. Transcripts of zebrafish abcb4 were detected in all of adult tissues examined. MC-LR was able to induce the expression of abcb4 gene and overexpression of Abcb4 significantly decreased the cytotoxicity and accumulation of MC-LR in LLC-PK1 cells and developing embryos. In contrast, overexpression of an Abcb4-G1177D mutant abolished its transporter function but not substrate binding activity, and sensitized LLC-PK1 cells and developing embryos to this cyanobacterial toxin. Moreover, ATPase activity in developing embryos can be induced by MC-LR. Thus, zebrafish Abcb4 plays crucial roles in cellular efflux of MC-LR and is a potential molecular marker for the monitoring of cyanobacteria contamination in the aquatic environment. (C) 2014 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordAbcb4 Efflux Transporter Microcystin-lr Detoxification
Department[Lu, Xing ; Sun, Rongze ; Lin, Li ; Zhong, Shan] Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China ; [Long, Yong ; Zhou, Bolan ; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
DOI10.1016/j.cbpc.2014.08.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS KeywordP-GLYCOPROTEIN ; EXPRESSION ; RESISTANCE ; TOXICITY ; EMBRYOS ; CELLS ; FISH ; GENE ; CYANOBACTERIA ; MDR1
WOS IDWOS:000348825300005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23991
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorZhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China.
Affiliation1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Lu, Xing,Long, Yong,Sun, Rongze,et al. Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2015,167:35-42.
APA Lu, Xing.,Long, Yong.,Sun, Rongze.,Zhou, Bolan.,Lin, Li.,...&Zhong, S .(2015).Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,167,35-42.
MLA Lu, Xing,et al."Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 167(2015):35-42.
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