中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR
作者: Lu, Xing1; Long, Yong2; Sun, Rongze1; Zhou, Bolan2; Lin, Li1; Zhong, Shan1; Cui, Zongbin2
通讯作者: Zhong, S (reprint author), Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China.
关键词: Abcb4 ; Efflux transporter ; Microcystin-LR ; Detoxification
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
发表日期: 2015
DOI: 10.1016/j.cbpc.2014.08.005
卷: 167, 页:35-42
收录类别: SCI
文章类型: Article
部门归属: [Lu, Xing ; Sun, Rongze ; Lin, Li ; Zhong, Shan] Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China ; [Long, Yong ; Zhou, Bolan ; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
英文摘要: Microcystin-LR (MC-LR) is one of the most common microcystins (MCs), which are hepatotoxic and released into a water body during a period of cyanobacterial blooms. These toxicants can be accumulated in aquatic animals and transferred along the food chain and thus pose adverse effects on aquatic environment and public health. Zebrafish Abcb4 is reported to mediate the cellular efflux of ecotoxicologically relevant compounds including galaxolide, tonalide and phenanthrene; however, it remains unclear whether Abcb4 functions in the detoxification of MC-LR. Here, we demonstrated the role of zebrafish Abcb4 in cellular efflux of MC-LR. Transcripts of zebrafish abcb4 were detected in all of adult tissues examined. MC-LR was able to induce the expression of abcb4 gene and overexpression of Abcb4 significantly decreased the cytotoxicity and accumulation of MC-LR in LLC-PK1 cells and developing embryos. In contrast, overexpression of an Abcb4-G1177D mutant abolished its transporter function but not substrate binding activity, and sensitized LLC-PK1 cells and developing embryos to this cyanobacterial toxin. Moreover, ATPase activity in developing embryos can be induced by MC-LR. Thus, zebrafish Abcb4 plays crucial roles in cellular efflux of MC-LR and is a potential molecular marker for the monitoring of cyanobacteria contamination in the aquatic environment. (C) 2014 Elsevier Inc. All rights reserved.
关键词[WOS]: P-GLYCOPROTEIN ; EXPRESSION ; RESISTANCE ; TOXICITY ; EMBRYOS ; CELLS ; FISH ; GENE ; CYANOBACTERIA ; MDR1
语种: 英语
WOS记录号: WOS:000348825300005
ISSN号: 1532-0456
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23991
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR.pdf(1281KB)----开放获取View Download

作者单位: 1.Wuhan Univ, Sch Basic Med Sci, Dept Genet, Wuhan 430071, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Lu, Xing]'s Articles
[Long, Yong]'s Articles
[Sun, Rongze]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Lu, Xing]‘s Articles
[Long, Yong]‘s Articles
[Sun, Rongze]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Zebrafish Abcb4 is a potential efflux transporter of microcystin-LR.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace