IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-kappa B, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine
Wang, Biao1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Wu, Pei1; Kuang, Sheng-Yao4; Jiang, Jun1,2,3; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Liu, Yang1,2,3; Zhou, Xiao-Qiu1,2,3; Zhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.
2015
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume158Issue:*Pages:125-137
AbstractThis study explored the possible preventive effects of dietary arginine on copper (Cu)-induced tight junction mRNA expression changes, apoptosis and antioxidant responses in the gills of young grass carp (Ctenopharyngodon idella). The results indicated that exposure to 0.7 mg/L (11.01 mu mol/L) Cu for 96h induced the production of reactive oxygen species (ROS), thereby increasing protein oxidation, lipid peroxidation and DNA damage in the gills of fish. However, these oxidative effects were prevented by arginine supplementation. Arginine also prevented the toxic effects of Cu on the activities of copper/zinc superoxide dismutase (SOD1), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and the glutathione (GSH) content (P<0.05). However, Cu induced an adaptive increase in the activity of catalase (CAT), and arginine supplementation further increased CAT activity (P<0.05). Moreover, Cu induced increases in the relative mRNA expressions of SOD1, CAT, GPx, GST, caspase-3, caspase-9, NF-E2-related factor 2 (Nrf2), Kelch-like-ECH-associated protein la (Keap1a), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-8 (IL-8), transforming growth factor-beta (TGF-beta) and nuclear transcription factor-kappa B p65 (NF-kappa B p65) in the gills of grass carp (P< 0.05). In contrast, the relative mRNA expression levels of occludin, zonula occludens-1 (ZO-1), claudin b, claudin 3, claudin 12, target of rapamycin (TOR) and inhibitor factor kappa B alpha (I kappa B alpha) in the gills were decreased by Cu (P< 0.05). However, pre-treatment of fish with arginine prevented Cu-induced relative mRNA expression decrease. Interestingly, Cu exposure resulted in increases in claudin 15a mRNA expression (P< 0.05) but could not induce claudin c, caspase-8 and interleukin-10 (IL-10) mRNA expression changes in the gill of fish (P>0.05). These results indicated that Cu exposure induced apoptosis and antioxidant system and tight junction mRNA changes in the fish gills, which could be completely blocked by dietary arginine pre-supplementation. (C) 2014 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordCopper Gill Tight Junction Apoptosis Signaling Molecule Arginine
Department[Wang, Biao ; Feng, Lin ; Jiang, Wei-Dan ; Wu, Pei ; Jiang, Jun ; Liu, Yang ; Zhou, Xiao-Qiu] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China ; [Feng, Lin ; Jiang, Wei-Dan ; Jiang, Jun ; Liu, Yang ; Zhou, Xiao-Qiu] Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China ; [Feng, Lin ; Jiang, Wei-Dan ; Jiang, Jun ; Liu, Yang ; Zhou, Xiao-Qiu] Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China ; [Kuang, Sheng-Yao ; Tang, Ling ; Tang, Wu-Neng] Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China ; [Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1016/j.aquatox.2014.10.025
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000348888400014
WOS KeywordRAINBOW-TROUT GILL ; OXIDATIVE STRESS ; DOWN-REGULATION ; PERMEABILITY ; CELLS ; MYOINOSITOL ; PROTEINS ; BARRIER ; SYSTEM ; DAMAGE
Citation statistics
Cited Times:52[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23990
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Biao,Feng, Lin,Jiang, Wei-Dan,et al. Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-kappa B, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine[J]. AQUATIC TOXICOLOGY,2015,158(*):125-137.
APA Wang, Biao.,Feng, Lin.,Jiang, Wei-Dan.,Wu, Pei.,Kuang, Sheng-Yao.,...&Zhou, XQ .(2015).Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-kappa B, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine.AQUATIC TOXICOLOGY,158(*),125-137.
MLA Wang, Biao,et al."Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-kappa B, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine".AQUATIC TOXICOLOGY 158.*(2015):125-137.
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