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题名: Copper-induced tight junction mRNA expression changes, apoptosis and antioxidant responses via NF-kappa B, TOR and Nrf2 signaling molecules in the gills of fish: Preventive role of arginine
作者: Wang, Biao1; Feng, Lin1, 2, 3; Jiang, Wei-Dan1, 2, 3; Wu, Pei1; Kuang, Sheng-Yao4; Jiang, Jun1, 2, 3; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5; Liu, Yang1, 2, 3; Zhou, Xiao-Qiu1, 2, 3
通讯作者: Zhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.
关键词: Copper ; Gill ; Tight junction ; Apoptosis ; Signaling molecule ; Arginine
刊名: AQUATIC TOXICOLOGY
发表日期: 2015
DOI: 10.1016/j.aquatox.2014.10.025
卷: 158, 期:*, 页:125-137
收录类别: SCI
文章类型: Article
部门归属: [Wang, Biao ; Feng, Lin ; Jiang, Wei-Dan ; Wu, Pei ; Jiang, Jun ; Liu, Yang ; Zhou, Xiao-Qiu] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China ; [Feng, Lin ; Jiang, Wei-Dan ; Jiang, Jun ; Liu, Yang ; Zhou, Xiao-Qiu] Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China ; [Feng, Lin ; Jiang, Wei-Dan ; Jiang, Jun ; Liu, Yang ; Zhou, Xiao-Qiu] Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China ; [Kuang, Sheng-Yao ; Tang, Ling ; Tang, Wu-Neng] Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China ; [Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
英文摘要: This study explored the possible preventive effects of dietary arginine on copper (Cu)-induced tight junction mRNA expression changes, apoptosis and antioxidant responses in the gills of young grass carp (Ctenopharyngodon idella). The results indicated that exposure to 0.7 mg/L (11.01 mu mol/L) Cu for 96h induced the production of reactive oxygen species (ROS), thereby increasing protein oxidation, lipid peroxidation and DNA damage in the gills of fish. However, these oxidative effects were prevented by arginine supplementation. Arginine also prevented the toxic effects of Cu on the activities of copper/zinc superoxide dismutase (SOD1), glutathione-S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and the glutathione (GSH) content (P<0.05). However, Cu induced an adaptive increase in the activity of catalase (CAT), and arginine supplementation further increased CAT activity (P<0.05). Moreover, Cu induced increases in the relative mRNA expressions of SOD1, CAT, GPx, GST, caspase-3, caspase-9, NF-E2-related factor 2 (Nrf2), Kelch-like-ECH-associated protein la (Keap1a), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-8 (IL-8), transforming growth factor-beta (TGF-beta) and nuclear transcription factor-kappa B p65 (NF-kappa B p65) in the gills of grass carp (P< 0.05). In contrast, the relative mRNA expression levels of occludin, zonula occludens-1 (ZO-1), claudin b, claudin 3, claudin 12, target of rapamycin (TOR) and inhibitor factor kappa B alpha (I kappa B alpha) in the gills were decreased by Cu (P< 0.05). However, pre-treatment of fish with arginine prevented Cu-induced relative mRNA expression decrease. Interestingly, Cu exposure resulted in increases in claudin 15a mRNA expression (P< 0.05) but could not induce claudin c, caspase-8 and interleukin-10 (IL-10) mRNA expression changes in the gill of fish (P>0.05). These results indicated that Cu exposure induced apoptosis and antioxidant system and tight junction mRNA changes in the fish gills, which could be completely blocked by dietary arginine pre-supplementation. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: RAINBOW-TROUT GILL ; OXIDATIVE STRESS ; DOWN-REGULATION ; PERMEABILITY ; CELLS ; MYOINOSITOL ; PROTEINS ; BARRIER ; SYSTEM ; DAMAGE
语种: 英语
WOS记录号: WOS:000348888400014
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23990
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
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