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题名: Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803
作者: Mo, Ran1, 2; Yang, Mingkun1; Chen, Zhuo1; Cheng, Zhongyi3; Yi, Xingling4; Li, Chongyang1; He, Chenliu1; Xiong, Qian1; Chen, Hui1; Wang, Qiang1; Ge, Feng1
通讯作者: Wang, Q (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
关键词: post-translational modification ; acetylome ; cyanobacterium ; Synecystis photosynthesis ; carbon metabolism
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2015-02-01
DOI: 10.1021/pr501275a
卷: 14, 期:2, 页:1275-1286
收录类别: SCI
文章类型: Article
部门归属: [Mo, Ran ; Yang, Mingkun ; Chen, Zhuo ; Li, Chongyang ; He, Chenliu ; Xiong, Qian ; Chen, Hui ; Wang, Qiang ; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Mo, Ran] Univ Chinese Acad Sci, Beijing 100039, Peoples R China ; [Cheng, Zhongyi] Tongji Univ, Adv Inst Translat Med, Shanghai 200092, Peoples R China ; [Yi, Xingling] Jingjie PTM Biolabs Hangzhou Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Cyanobacteria are the oldest known life form inhabiting Earth and the only prokaryotes capable of performing oxygenic photosynthesis. Synechocystis sp. PCC 6803 (Synechocystis) is a model cyanobacterium used extensively in research on photosynthesis and environmental adaptation. Posttranslational protein modification by lysine acetylation plays a critical regulatory role in both eukaryotes and prokaryotes; however, its extent and function in cyanobacteria remain unexplored. Herein, we performed a global acetylome analysis on Synechocystis through peptide prefractionation, antibody enrichment, and high accuracy LC-MS/MS analysis; identified 776 acetylation sites on 513 acetylated proteins; and functionally categorized them into an interaction map showing their involvement in various biological processes. Consistent with previous reports, a large fraction of the acetylation sites are present on proteins involved in cellular metabolism. Interestingly, for the first time, many proteins involved in photosynthesis, including the subunits of phycocyanin (CpcA, CpcB, CpcC, and CpcG) and allophycocyanin (ApcA, ApcB, ApcD, ApcE, and ApcF), were found to be lysine acetylated, suggesting that lysine acetylation may play regulatory roles in the photosynthesis process. Six identified acetylated proteins associated with photosynthesis and carbon metabolism were further validated by immunoprecipitation and Western blotting. Our data provide the first global survey of lysine acetylation in cyanobacteria and reveal previously unappreciated roles of lysine acetylation in the regulation of photosynthesis. The provided data set may serve as an important resource for the functional analysis of lysine acetylation in cyanobacteria and facilitate the elucidation of the entire metabolic networks and photosynthesis process in this model cyanobacterium.
关键词[WOS]: BACTERIUM BACILLUS-SUBTILIS ; ESCHERICHIA-COLI ; MASS-SPECTROMETRY ; PHOTOSYSTEM-II ; SP PCC-6803 ; POSTTRANSLATIONAL MODIFICATIONS ; INTERACTION NETWORKS ; PROTEIN MODIFICATION ; HISTONE ACETYLATION ; CROSS-TALK
语种: 英语
WOS记录号: WOS:000349276400060
ISSN号: 1535-3893
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23987
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Tongji Univ, Adv Inst Translat Med, Shanghai 200092, Peoples R China
4.Jingjie PTM Biolabs Hangzhou Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China
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