Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803
Mo, Ran1,2; Yang, Mingkun1; Chen, Zhuo1; Cheng, Zhongyi3; Yi, Xingling4; Li, Chongyang1; He, Chenliu1; Xiong, Qian1; Chen, Hui1; Wang, Qiang1; Ge, Feng1; Wang, Q (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2015-02-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume14Issue:2Pages:1275-1286
AbstractCyanobacteria are the oldest known life form inhabiting Earth and the only prokaryotes capable of performing oxygenic photosynthesis. Synechocystis sp. PCC 6803 (Synechocystis) is a model cyanobacterium used extensively in research on photosynthesis and environmental adaptation. Posttranslational protein modification by lysine acetylation plays a critical regulatory role in both eukaryotes and prokaryotes; however, its extent and function in cyanobacteria remain unexplored. Herein, we performed a global acetylome analysis on Synechocystis through peptide prefractionation, antibody enrichment, and high accuracy LC-MS/MS analysis; identified 776 acetylation sites on 513 acetylated proteins; and functionally categorized them into an interaction map showing their involvement in various biological processes. Consistent with previous reports, a large fraction of the acetylation sites are present on proteins involved in cellular metabolism. Interestingly, for the first time, many proteins involved in photosynthesis, including the subunits of phycocyanin (CpcA, CpcB, CpcC, and CpcG) and allophycocyanin (ApcA, ApcB, ApcD, ApcE, and ApcF), were found to be lysine acetylated, suggesting that lysine acetylation may play regulatory roles in the photosynthesis process. Six identified acetylated proteins associated with photosynthesis and carbon metabolism were further validated by immunoprecipitation and Western blotting. Our data provide the first global survey of lysine acetylation in cyanobacteria and reveal previously unappreciated roles of lysine acetylation in the regulation of photosynthesis. The provided data set may serve as an important resource for the functional analysis of lysine acetylation in cyanobacteria and facilitate the elucidation of the entire metabolic networks and photosynthesis process in this model cyanobacterium.
SubtypeArticle
KeywordPost-translational Modification Acetylome Cyanobacterium Synecystis Photosynthesis Carbon Metabolism
Department[Mo, Ran ; Yang, Mingkun ; Chen, Zhuo ; Li, Chongyang ; He, Chenliu ; Xiong, Qian ; Chen, Hui ; Wang, Qiang ; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Mo, Ran] Univ Chinese Acad Sci, Beijing 100039, Peoples R China ; [Cheng, Zhongyi] Tongji Univ, Adv Inst Translat Med, Shanghai 200092, Peoples R China ; [Yi, Xingling] Jingjie PTM Biolabs Hangzhou Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China
DOI10.1021/pr501275a
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS KeywordBACTERIUM BACILLUS-SUBTILIS ; ESCHERICHIA-COLI ; MASS-SPECTROMETRY ; PHOTOSYSTEM-II ; SP PCC-6803 ; POSTTRANSLATIONAL MODIFICATIONS ; INTERACTION NETWORKS ; PROTEIN MODIFICATION ; HISTONE ACETYLATION ; CROSS-TALK
WOS IDWOS:000349276400060
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23987
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorWang, Q (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Tongji Univ, Adv Inst Translat Med, Shanghai 200092, Peoples R China
4.Jingjie PTM Biolabs Hangzhou Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Mo, Ran,Yang, Mingkun,Chen, Zhuo,et al. Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(2):1275-1286.
APA Mo, Ran.,Yang, Mingkun.,Chen, Zhuo.,Cheng, Zhongyi.,Yi, Xingling.,...&Wang, Q .(2015).Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803.JOURNAL OF PROTEOME RESEARCH,14(2),1275-1286.
MLA Mo, Ran,et al."Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803".JOURNAL OF PROTEOME RESEARCH 14.2(2015):1275-1286.
Files in This Item:
File Name/Size DocType Version Access License
Acetylome Analysis R(6796KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Mo, Ran]'s Articles
[Yang, Mingkun]'s Articles
[Chen, Zhuo]'s Articles
Baidu academic
Similar articles in Baidu academic
[Mo, Ran]'s Articles
[Yang, Mingkun]'s Articles
[Chen, Zhuo]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Mo, Ran]'s Articles
[Yang, Mingkun]'s Articles
[Chen, Zhuo]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Acetylome Analysis Reveals the Involvement of Lysine Acetylation in Photosynthesis and Carbon Metabolism in the Model Cyanobacterium Synechocystis sp PCC 6803.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.