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Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane
Gao, Shan1,2; Gu, Wenhui1,2; Xiong, Qian3; Ge, Feng3; Xie, Xiujun4; Li, Jian5; Chen, Weizhou6; Pan, Guanghua4; Wang, Guangce1; Wang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
2015-03-01
Source PublicationPHYSIOLOGIA PLANTARUM
ISSN0031-9317
Volume153Issue:3Pages:492-502
AbstractDesiccation has significant effects on photosynthetic processes in intertidal macro-algae. We studied an intertidal macro-alga, Ulva sp., which can tolerate desiccation, to investigate changes in photosynthetic performance and the components and structure of thylakoid membrane proteins in response to desiccation. Our results demonstrate that photosystem II (PSII) is more sensitive to desiccation than photosystem I (PSI) in Ulva sp. Comparative proteomics of the thylakoid membrane proteins at different levels of desiccation suggested that there were few changes in the content of proteins involved in photosynthesis during desiccation. Interestingly, we found that both the PSII subunit, PsbS (Photosystem II S subunit) (a four-helix protein in the LHC superfamily), and light-harvesting complex stress-related (LHCSR) proteins, which are required for non-photochemical quenching in land plants and algae, respectively, were present under both normal and desiccation conditions and both increased slightly during desiccation. In addition, the results of immunoblot analysis suggested that the phosphorylation of PSII and LHCII increases during desiccation. To investigate further, we separated out a supercomplex formed during desiccation by blue native-polyacrylamide gel electrophoresis and identified the components by mass spectrometry analysis. Our results show that phosphorylation of the complex increases slightly with decreased water content. All the results suggest that during the course of desiccation, few changes occur in the content of thylakoid membrane proteins, but a rearrangement of the protein complex occurs in the intertidal macro-alga Ulva sp.
SubtypeArticle
Department[Gao, Shan ; Gu, Wenhui ; Wang, Guangce] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China ; [Gao, Shan ; Gu, Wenhui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Xiong, Qian ; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Xie, Xiujun ; Pan, Guanghua] Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China ; [Li, Jian] Catholic Univ Louvain, Earth & Life Inst, B-1348 Louvain La Neuve, Belgium ; [Chen, Weizhou] Shantou Univ, Inst Marine Biol, Shantou 515063, Peoples R China
DOI10.1111/ppl.12258
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000349969500013
WOS KeywordLIGHT-HARVESTING COMPLEX ; CYCLIC ELECTRON FLOW ; PHOTOSYSTEM-II ; CHLAMYDOMONAS-REINHARDTII ; PHOTOSYNTHETIC MEMBRANES ; DEPENDENT REGULATION ; ARABIDOPSIS ; TEMPERATURE ; DEFICIENCY ; MECHANISMS
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23986
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorWang, GC (reprint author), Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
4.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
5.Catholic Univ Louvain, Earth & Life Inst, B-1348 Louvain La Neuve, Belgium
6.Shantou Univ, Inst Marine Biol, Shantou 515063, Peoples R China
Recommended Citation
GB/T 7714
Gao, Shan,Gu, Wenhui,Xiong, Qian,et al. Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane[J]. PHYSIOLOGIA PLANTARUM,2015,153(3):492-502.
APA Gao, Shan.,Gu, Wenhui.,Xiong, Qian.,Ge, Feng.,Xie, Xiujun.,...&Wang, GC .(2015).Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane.PHYSIOLOGIA PLANTARUM,153(3),492-502.
MLA Gao, Shan,et al."Desiccation enhances phosphorylation of PSII and affects the distribution of protein complexes in the thylakoid membrane".PHYSIOLOGIA PLANTARUM 153.3(2015):492-502.
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