Sufficient Numbers of Early Germ Cells Are Essential for Female Sex Development in Zebrafish
Dai, Xiangyan1,2; Jin, Xia1; Chen, Xiaowen1; He, Jiangyan1; Yin, Zhan1; Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
2015-02-13
Source PublicationPLOS ONE
ISSN1932-6203
Volume10Issue:2Pages:e0117824
AbstractThe sex determination for zebrafish is controlled by a combination of genetic and environmental factors. The determination of sex in zebrafish has been suggested to rely on a mechanism that is affected by germ cell-derived signals. To begin our current study, a simplified and efficient germ cell-specific promoter of the dead end (dnd) gene was identified. Utilizing the metrodinazole (MTZ)/bacterial nitroreductase (NTR) system for inducible germ cell ablation, several stable Tg (dnd:NTR-EGFP(-3'UTR)) and Tg (dnd:NTR-EGFP(+3'UTR)) zebrafish lines were then generated with the identified promoter. A thorough comparison of the expression patterns and tissue distributions of endogenous dnd and ntr-egfp transcripts in vivo revealed that the identified 2032-bp zebrafish dnd promoter can recapitulate dnd expression faithfully in stable transgenic zebrafish. The correlation between the levels of the germ cell-derived signals and requirement for maintaining the female fate has been also explored with different durations of the MTZ treatments. Our results revealed the decreasing ratios of female presented in the treated transgenic group are fairly associated with the reducing levels of the early germ cell-derived signals. After the juvenile transgenic fish treated with 5 mM MTZ for 20 days, all MTZ-treated transgenic fish exclusively developed into males with subfertilities. Taken together, our results identified here a simplified and efficient dnd promoter, and provide clear evidence indicating that it was not the presence but the sufficiency of signals derived from germ cells that is essential for female sex development in zebrafish. Our model also provides a unique system for sex control in zebrafish studies.
SubtypeArticle
Department[Dai, Xiangyan ; Jin, Xia ; Chen, Xiaowen ; He, Jiangyan ; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China ; [Dai, Xiangyan] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1371/journal.pone.0117824
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS KeywordDANIO-RERIO ; DEAD-END ; GENE-EXPRESSION ; ADULT ZEBRAFISH ; TELEOST FISH ; DIFFERENTIATION ; LINE ; REGENERATION ; PROMOTER ; GONAD
WOS IDWOS:000350682600092
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23985
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorYin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Dai, Xiangyan,Jin, Xia,Chen, Xiaowen,et al. Sufficient Numbers of Early Germ Cells Are Essential for Female Sex Development in Zebrafish[J]. PLOS ONE,2015,10(2):e0117824.
APA Dai, Xiangyan,Jin, Xia,Chen, Xiaowen,He, Jiangyan,Yin, Zhan,&Yin, Z .(2015).Sufficient Numbers of Early Germ Cells Are Essential for Female Sex Development in Zebrafish.PLOS ONE,10(2),e0117824.
MLA Dai, Xiangyan,et al."Sufficient Numbers of Early Germ Cells Are Essential for Female Sex Development in Zebrafish".PLOS ONE 10.2(2015):e0117824.
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