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题名: ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena
作者: Ning YingZhi1; Dang Huai1; Liu GuangLong1; Xiong Jie2; Yuan DongXia2; Feng LiFang3; Miao Wei2
通讯作者: Feng, LF (reprint author), Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China.
关键词: Tetrahymena ; DDT ; ATP-binding cassette transporter ; tolerance
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2015-03-01
DOI: 10.1007/s11427-014-4743-x
卷: 58, 期:3, 页:297-304
收录类别: SCI
文章类型: Article
部门归属: [Ning YingZhi ; Dang Huai ; Liu GuangLong] Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China ; [Xiong Jie ; Yuan DongXia ; Miao Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Feng LiFang] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
英文摘要: The reuse of dichlorodiphenyltrichloroethane (DDT) as an indoor residual spray was permitted by the World Health Organization in 2007, and approximately 14 countries still use DDT to control disease vectors. The extensive exposure of insects to DDT has resulted in the emergence of DDT resistance, especially in mosquitoes, and the mechanism for this resistance in mosquitoes has been widely reported. Spraying can also introduce DDT directly into surface water, and DDT can subsequently accumulate in microorganisms, but the mechanism for the resistance to DDT degradation in microorganisms is unclear. Using whole-genome microarray analysis, we detected an abcb15 gene that was up-regulated in a specific manner by DDT treatment in T. thermophile. The deduced ABCB15 peptide sequence had two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs) to form the structure TMD-NBD-TMD-NBD, and each NBD contained three conserved motifs: Walker-A, C-loop, and Walker-B, which indicated the T. thermophila abcb15 was a typical ABC transporter gene. The expression of ABCB15 fused with a C-terminal green fluorescent protein was found to be on the periphery of the cell, suggesting that ABCB15 was a membrane pump protein. In addition, cells with abcb15 partially knocked down (abcb15-KD) grew slower than wild-type cells in the presence of 256 mg L-1 DDT, indicating the tolerance of abcb15-KD strain to DDT exposure was decreased. Thus, we suggest that in Tetrahymena, the membrane pump protein encoded by ABCT gene abcb15 can enhance the tolerance to DDT and protect cells from this exogenous toxin by efficiently pumping it to the extracellular space.
关键词[WOS]: SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; MULTIDRUG-RESISTANCE ; ABC-TRANSPORTERS ; P-GLYCOPROTEIN ; GENE-EXPRESSION ; TRIBUTYLTIN TBT ; THERMOPHILA ; IDENTIFICATION ; TRANSFORMATION
语种: 英语
WOS记录号: WOS:000351809200010
ISSN号: 1674-7305
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23984
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China
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