ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena
Ning YingZhi1; Dang Huai1; Liu GuangLong1; Xiong Jie2; Yuan DongXia2; Feng LiFang3; Miao Wei2; Feng, LF (reprint author), Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China.
2015-03-01
Source PublicationSCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
Volume58Issue:3Pages:297-304
AbstractThe reuse of dichlorodiphenyltrichloroethane (DDT) as an indoor residual spray was permitted by the World Health Organization in 2007, and approximately 14 countries still use DDT to control disease vectors. The extensive exposure of insects to DDT has resulted in the emergence of DDT resistance, especially in mosquitoes, and the mechanism for this resistance in mosquitoes has been widely reported. Spraying can also introduce DDT directly into surface water, and DDT can subsequently accumulate in microorganisms, but the mechanism for the resistance to DDT degradation in microorganisms is unclear. Using whole-genome microarray analysis, we detected an abcb15 gene that was up-regulated in a specific manner by DDT treatment in T. thermophile. The deduced ABCB15 peptide sequence had two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs) to form the structure TMD-NBD-TMD-NBD, and each NBD contained three conserved motifs: Walker-A, C-loop, and Walker-B, which indicated the T. thermophila abcb15 was a typical ABC transporter gene. The expression of ABCB15 fused with a C-terminal green fluorescent protein was found to be on the periphery of the cell, suggesting that ABCB15 was a membrane pump protein. In addition, cells with abcb15 partially knocked down (abcb15-KD) grew slower than wild-type cells in the presence of 256 mg L-1 DDT, indicating the tolerance of abcb15-KD strain to DDT exposure was decreased. Thus, we suggest that in Tetrahymena, the membrane pump protein encoded by ABCT gene abcb15 can enhance the tolerance to DDT and protect cells from this exogenous toxin by efficiently pumping it to the extracellular space.
SubtypeArticle
KeywordTetrahymena Ddt Atp-binding Cassette Transporter Tolerance
Department[Ning YingZhi ; Dang Huai ; Liu GuangLong] Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China ; [Xiong Jie ; Yuan DongXia ; Miao Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Feng LiFang] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China
DOI10.1007/s11427-014-4743-x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS KeywordSUPPRESSION SUBTRACTIVE HYBRIDIZATION ; DICHLORODIPHENYLTRICHLOROETHANE DDT ; MULTIDRUG-RESISTANCE ; ABC-TRANSPORTERS ; P-GLYCOPROTEIN ; GENE-EXPRESSION ; TRIBUTYLTIN TBT ; THERMOPHILA ; IDENTIFICATION ; TRANSFORMATION
WOS IDWOS:000351809200010
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23984
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorFeng, LF (reprint author), Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China.
Affiliation1.Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Key Lab Food Safety Zhejiang Prov, Hangzhou 310018, Peoples R China
Recommended Citation
GB/T 7714
Ning YingZhi,Dang Huai,Liu GuangLong,et al. ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena[J]. SCIENCE CHINA-LIFE SCIENCES,2015,58(3):297-304.
APA Ning YingZhi.,Dang Huai.,Liu GuangLong.,Xiong Jie.,Yuan DongXia.,...&Feng, LF .(2015).ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena.SCIENCE CHINA-LIFE SCIENCES,58(3),297-304.
MLA Ning YingZhi,et al."ATP-binding cassette transporter enhances tolerance to DDT in Tetrahymena".SCIENCE CHINA-LIFE SCIENCES 58.3(2015):297-304.
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