[Yang, Xiaojie
; Gu, Qilin
; Li, Qing
; Cui, Zongbin] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China
; [Yang, Xiaojie
; Gu, Qilin] Univ Chinese Acad Sci, Beijing, Peoples R China
; [Lin, Li
; Li, Shaoyang
; Zhong, Shan] Wuhan Univ, Sch Basic Med Sci, Wuhan 430072, Peoples R China
WOS标题词:
Science & Technology
; Life Sciences & Biomedicine
类目[WOS]:
Biochemistry & Molecular Biology
; Cell Biology
研究领域[WOS]:
Biochemistry & Molecular Biology
; Cell Biology
英文摘要:
Nucleoporin p62 (Nup62) localizes in the central channel of nuclear pore complexes (NPCs) and regulates nuclear pore permeability and nucleocytoplasmic transport. However, the developmental roles of Nup62 in vertebrates remain largely unclear. Zebrafish Nup62-like protein (Nup62l) is a homolog of mammalian Nup62. The nup62l gene is maternally expressed, but its transcripts are ubiquitously distributed during early embryogenesis and enriched in the head, pharynx, and intestine of developing embryos. Activation of the Wnt/beta-catenin pathway positively modulates nup62l transcription, while Bmp signaling acts downstream of Wnt/b-catenin signaling to negatively regulate nup62l expression. Overexpression of nup62l dorsalized embryos and enhanced gastrula convergence and extension (CE) movements. In contrast, knockdown of Nup62l led to ventralized embryos, an impediment to CE movements, and defects in specification of midline organ progenitors. Mechanistically, Nup62l acts as an activator of Wnt/beta-catenin signaling through interaction with and facilitation of nuclear import of beta-catenin-1/2 in zebrafish. Thus, Nup62l regulates dorsoventral patterning, gastrula CE movements, and proper specification of midline organ precursors through mediating the nuclear import of beta-catenins in zebrafish.
1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Wuhan Univ, Sch Basic Med Sci, Wuhan 430072, Peoples R China
