中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress
作者: Xiong, Qian1; Feng, Jie1, 2; Li, Si-ting1, 2; Zhang, Gui-ying1, 2; Qiao, Zhi-xian1; Chen, Zhuo1; Wu, Ying1, 2; Lin, Yan1; Li, Tao1; Ge, Feng1; Zhao, Jin-dong1
通讯作者: Li, T (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2015-04-01
DOI: 10.1074/mcp.M114.046003
卷: 14, 期:4, 页:1038-1053
收录类别: SCI
文章类型: Article
部门归属: [Xiong, Qian ; Feng, Jie ; Li, Si-ting ; Zhang, Gui-ying ; Qiao, Zhi-xian ; Chen, Zhuo ; Wu, Ying ; Lin, Yan ; Li, Tao ; Ge, Feng ; Zhao, Jin-dong] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Feng, Jie ; Li, Si-ting ; Zhang, Gui-ying ; Wu, Ying] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Sufficient light is essential for the growth and physiological functions of photosynthetic organisms, but prolonged exposure to high light (HL) stress can cause cellular damage and ultimately result in the death of these organisms. Synechococcus sp. PCC 7002 (hereafter Synechococcus 7002) is a unicellular cyanobacterium with exceptional tolerance to HL intensities. However, the molecular mechanisms involved in HL response by Synechococcus 7002 are not well understood. Here, an integrated RNA sequencing transcriptomic and quantitative proteomic analysis was performed to investigate the cellular response to HL in Synechococcus 7002. A total of 526 transcripts and 233 proteins were identified to be differentially regulated under HL stress. Data analysis revealed major changes in mRNAs and proteins involved in the photosynthesis pathways, resistance to light-induced damage, DNA replication and repair, and energy metabolism. A set of differentially expressed mRNAs and proteins were validated by quantitative RT-PCR and Western blot, respectively. Twelve genes differentially regulated under HL stress were selected for knockout generation and growth analysis of these mutants led to the identification of key genes involved in the response of HL in Synechococcus 7002. Taken altogether, this study established a model for global response mechanisms to HL in Synechococcus 7002 and may be valuable for further studies addressing HL resistance in photosynthetic organisms.
关键词[WOS]: SYNECHOCYSTIS SP PCC-6803 ; BLUE-GREEN-ALGAE ; STRAIN PCC 7002 ; ORANGE CAROTENOID PROTEIN ; PHOTOSYSTEM-II ; INDUCIBLE POLYPEPTIDES ; OXIDATIVE STRESS ; GENE-EXPRESSION ; PHOTOSYNTHETIC CHARACTERISTICS ; DELETION MUTAGENESIS
语种: 英语
WOS记录号: WOS:000352194400018
ISSN号: 1535-9476
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23982
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress.pdf(4452KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xiong, Qian]'s Articles
[Feng, Jie]'s Articles
[Li, Si-ting]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xiong, Qian]‘s Articles
[Feng, Jie]‘s Articles
[Li, Si-ting]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace