Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress
Xiong, Qian1; Feng, Jie1,2; Li, Si-ting1,2; Zhang, Gui-ying1,2; Qiao, Zhi-xian1; Chen, Zhuo1; Wu, Ying1,2; Lin, Yan1; Li, Tao1; Ge, Feng1; Zhao, Jin-dong1; Li, T (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2015-04-01
Source PublicationMOLECULAR & CELLULAR PROTEOMICS
ISSN1535-9476
Volume14Issue:4Pages:1038-1053
AbstractSufficient light is essential for the growth and physiological functions of photosynthetic organisms, but prolonged exposure to high light (HL) stress can cause cellular damage and ultimately result in the death of these organisms. Synechococcus sp. PCC 7002 (hereafter Synechococcus 7002) is a unicellular cyanobacterium with exceptional tolerance to HL intensities. However, the molecular mechanisms involved in HL response by Synechococcus 7002 are not well understood. Here, an integrated RNA sequencing transcriptomic and quantitative proteomic analysis was performed to investigate the cellular response to HL in Synechococcus 7002. A total of 526 transcripts and 233 proteins were identified to be differentially regulated under HL stress. Data analysis revealed major changes in mRNAs and proteins involved in the photosynthesis pathways, resistance to light-induced damage, DNA replication and repair, and energy metabolism. A set of differentially expressed mRNAs and proteins were validated by quantitative RT-PCR and Western blot, respectively. Twelve genes differentially regulated under HL stress were selected for knockout generation and growth analysis of these mutants led to the identification of key genes involved in the response of HL in Synechococcus 7002. Taken altogether, this study established a model for global response mechanisms to HL in Synechococcus 7002 and may be valuable for further studies addressing HL resistance in photosynthetic organisms.
SubtypeArticle
Department[Xiong, Qian ; Feng, Jie ; Li, Si-ting ; Zhang, Gui-ying ; Qiao, Zhi-xian ; Chen, Zhuo ; Wu, Ying ; Lin, Yan ; Li, Tao ; Ge, Feng ; Zhao, Jin-dong] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Feng, Jie ; Li, Si-ting ; Zhang, Gui-ying ; Wu, Ying] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
DOI10.1074/mcp.M114.046003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS KeywordSYNECHOCYSTIS SP PCC-6803 ; BLUE-GREEN-ALGAE ; STRAIN PCC 7002 ; ORANGE CAROTENOID PROTEIN ; PHOTOSYSTEM-II ; INDUCIBLE POLYPEPTIDES ; OXIDATIVE STRESS ; GENE-EXPRESSION ; PHOTOSYNTHETIC CHARACTERISTICS ; DELETION MUTAGENESIS
WOS IDWOS:000352194400018
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23982
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorLi, T (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Xiong, Qian,Feng, Jie,Li, Si-ting,et al. Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(4):1038-1053.
APA Xiong, Qian.,Feng, Jie.,Li, Si-ting.,Zhang, Gui-ying.,Qiao, Zhi-xian.,...&Li, T .(2015).Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress.MOLECULAR & CELLULAR PROTEOMICS,14(4),1038-1053.
MLA Xiong, Qian,et al."Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress".MOLECULAR & CELLULAR PROTEOMICS 14.4(2015):1038-1053.
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