IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila
Chen, Gangfu1; Liu, Yang1,2,3; Jiang, Jun1,2,3; Jiang, Weidan1,2,3; Kuang, Shengyao1,2,3; Tang, Ling1,4; Tang, Wuneng1,4; Zhang, Yong-An5; Zhou, Xiaoqiu1,2,3; Feng, Lin1,2,3; Zhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Peoples R China.
2015-05-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume44Issue:1Pages:195-202
AbstractThis study was conducted to test the hypothesis that elevated dietary arginine enhances immunity of juvenile Jian carp (Cyprinus carpio var. Jian). Fish were fed graded levels of dietary arginine for 9 weeks. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila. Head kidney and spleen weights, as well as erythrocyte and leukocyte counts were significantly influenced by dietary arginine levels. A similar trend was also observed for hemagglutination titre, serum lysozyme activity, IgM concentration, C3 and C4 content. The highest survival rates following A. hydrophila infection were obtained in fish fed the diets containing arginine at 16.1-21.9 g/kg diet. Phagocytic activity of leukocytes was significantly enhanced by dietary arginine supplementation. In contrast, acid phosphatase activity significantly decreased with dietary arginine levels. Dietary arginine levels did not have a significant effect on the total iron-binding capacity. Gene expression of TNF-alpha and TGF-beta in head kidney significantly increased with dietary arginine levels up to 21.9 g/kg diet, and decreased thereafter. Fish fed the basal diet exhibited the highest IL-10 mRNA expression level. Gene expression of IL-1 beta and TOR increased with dietary arginine addition, reaching a plateau at 18.5 and 21.9 g arginine/kg diet, respectively. In spleen, higher IL-1 beta and TNF-alpha gene expressions were obtained in fish fed the diets containing 24.5 g arginine/kg diet than in fish fed the other dietary treatments. TGF-beta mRNA expression levels were significantly lower in fish fed the diets containing <= 21.9 g arginine/kg diet. IL-10 and TOR mRNA expression levels were lower in fish fed 16.1 g arginine/kg diet, while 4E-BP mRNA expression levels increased with dietary arginine levels up to 12.7 g/kg diet and decreased thereafter. Our results indicate that arginine has beneficial effects on regulating mRNA expression of inflammatory cytokines, as well as TOR and 4E-BP and improving humoral and cellular immunity, therefore enhancing disease resistance of fish. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordArginine Cyprinus Carpio Var. Jian Disease Resistance Immune Response
Department[Chen, Gangfu ; Liu, Yang ; Jiang, Jun ; Jiang, Weidan ; Kuang, Shengyao ; Tang, Ling ; Tang, Wuneng ; Zhou, Xiaoqiu ; Feng, Lin] Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Peoples R China ; [Liu, Yang ; Jiang, Jun ; Jiang, Weidan ; Kuang, Shengyao ; Zhou, Xiaoqiu ; Feng, Lin] Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Yaan 625014, Peoples R China ; [Liu, Yang ; Jiang, Jun ; Jiang, Weidan ; Kuang, Shengyao ; Zhou, Xiaoqiu ; Feng, Lin] Sichuan Agr Univ, Sichuan Univ Key Lab, Fish Nutr & Safety Prod, Yaan 625014, Peoples R China ; [Tang, Ling ; Tang, Wuneng] Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China ; [Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1016/j.fsi.2015.02.027
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000353743900023
WOS KeywordCATFISH ICTALURUS-PUNCTATUS ; NITRIC-OXIDE PRODUCTION ; CHANNEL CATFISH ; CYPRINUS-CARPIO ; RAINBOW-TROUT ; GROWTH-PERFORMANCE ; OXIDATIVE STRESS ; JUVENILE JIAN ; ASCORBIC-ACID ; GRADED-LEVELS
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23979
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Peoples R China.
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Yaan 625014, Peoples R China
2.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Yaan 625014, Peoples R China
3.Sichuan Agr Univ, Sichuan Univ Key Lab, Fish Nutr & Safety Prod, Yaan 625014, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Gangfu,Liu, Yang,Jiang, Jun,et al. Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(1):195-202.
APA Chen, Gangfu.,Liu, Yang.,Jiang, Jun.,Jiang, Weidan.,Kuang, Shengyao.,...&Zhou, XQ .(2015).Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,44(1),195-202.
MLA Chen, Gangfu,et al."Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 44.1(2015):195-202.
Files in This Item: Download All
File Name/Size DocType Version Access License
Effect of dietary ar(1390KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Gangfu]'s Articles
[Liu, Yang]'s Articles
[Jiang, Jun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Gangfu]'s Articles
[Liu, Yang]'s Articles
[Jiang, Jun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Gangfu]'s Articles
[Liu, Yang]'s Articles
[Jiang, Jun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Effect of dietary arginine on the immune response and gene expression in head kidney and spleen following infection of Jian carp with Aeromonas hydrophila.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.