| Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish | |
| Zhang, Xu-Jie1,2; Zhang, Xiang-Yang1,2; Zhang, Nu1,2; Guo, Xia1,2; Peng, Kai-Song1,3; Wu, Han1; Lu, Long-Feng1,2; Wu, Nan1; Chen, Dan-Dan1,2; Li, Shun1; Nie, Pin1; Zhang, Yong-An1; Zhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China. | |
| 2015-05-15 | |
| Source Publication | JOURNAL OF IMMUNOLOGY
 |
| ISSN | 0022-1767 |
| Volume | 194Issue:10Pages:4974-4987 |
| Abstract | Cathelicidin antimicrobial peptides (CAMPs) represent a crucial component of the innate immune system in vertebrates. Although widely studied in mammals, little is known about the structure and function of fish CAMPs. Further to the previous findings, two more cathelicidin genes and multiple transcripts from rainbow trout were identified in the present study. Interestingly, we found that trout have evolved energy-saving forms of cathelicidins with the total deletion of the characteristic cathelin-like domain. Sequence analysis revealed that salmonid CAMPs have formed a special class of antimicrobial peptides in vertebrates with three distinctive hallmarks: the N terminus is intensified by positive charges, the central region consists of repetitive motifs based on RPGGGS, and the C terminus is lowly charged. Immunofluorescence localization of trout CAMPs demonstrated that these peptides expressed mainly at the mucosal layer of gut. Meanwhile, signals around sinusoids were also detected in head kidney. Moreover, the biological activities of trout CAMPs were proved to be mediated by the N terminus. Additionally, the repetitive motifs characteristically existing in Salmonidae increased the structural flexibilities of peptides and further increased the antibacterial and IL-8-stimulating activities. Unlike most a helical and cytotoxic mammalian CAMPs, trout CAMPs, mainly consisting of beta-sheet and random coil, exhibited no cytotoxic activities. The distinctive structural features of trout CAMPs provide new insights into the understanding of the evolution of CAMPs in vertebrates. Moreover, the high bacterial membrane selectivity of trout CAMPs will help to design excellent peptide antibiotics. |
| Subtype | Article |
| Department | [Zhang, Xu-Jie ; Zhang, Xiang-Yang ; Zhang, Nu ; Guo, Xia ; Peng, Kai-Song ; Wu, Han ; Lu, Long-Feng ; Wu, Nan ; Chen, Dan-Dan ; Li, Shun ; Nie, Pin ; Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Zhang, Xu-Jie ; Zhang, Xiang-Yang ; Zhang, Nu ; Guo, Xia ; Lu, Long-Feng ; Chen, Dan-Dan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Peng, Kai-Song] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China |
| DOI | 10.4049/jimmunol.1500182 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Immunology |
| WOS Subject | Immunology |
| WOS Keyword | SECONDARY STRUCTURE ANALYSES ; FUNCTIONAL-CHARACTERIZATION ; ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; HOST-DEFENSE ; GENE FAMILY ; LL-37 ; EXPRESSION ; INNATE ; IDENTIFICATION |
| WOS ID | WOS:000353728800039 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/23978 |
| Collection | 藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心 |
| Corresponding Author | Zhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Xu-Jie,Zhang, Xiang-Yang,Zhang, Nu,et al. Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish[J]. JOURNAL OF IMMUNOLOGY,2015,194(10):4974-4987. |
| APA | Zhang, Xu-Jie.,Zhang, Xiang-Yang.,Zhang, Nu.,Guo, Xia.,Peng, Kai-Song.,...&Zhang, YA .(2015).Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish.JOURNAL OF IMMUNOLOGY,194(10),4974-4987. |
| MLA | Zhang, Xu-Jie,et al."Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish".JOURNAL OF IMMUNOLOGY 194.10(2015):4974-4987. |
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| Distinctive Structur（4144KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
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