Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish
Zhang, Xu-Jie1,2; Zhang, Xiang-Yang1,2; Zhang, Nu1,2; Guo, Xia1,2; Peng, Kai-Song1,3; Wu, Han1; Lu, Long-Feng1,2; Wu, Nan1; Chen, Dan-Dan1,2; Li, Shun1; Nie, Pin1; Zhang, Yong-An1; Zhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
2015-05-15
Source PublicationJOURNAL OF IMMUNOLOGY
ISSN0022-1767
Volume194Issue:10Pages:4974-4987
AbstractCathelicidin antimicrobial peptides (CAMPs) represent a crucial component of the innate immune system in vertebrates. Although widely studied in mammals, little is known about the structure and function of fish CAMPs. Further to the previous findings, two more cathelicidin genes and multiple transcripts from rainbow trout were identified in the present study. Interestingly, we found that trout have evolved energy-saving forms of cathelicidins with the total deletion of the characteristic cathelin-like domain. Sequence analysis revealed that salmonid CAMPs have formed a special class of antimicrobial peptides in vertebrates with three distinctive hallmarks: the N terminus is intensified by positive charges, the central region consists of repetitive motifs based on RPGGGS, and the C terminus is lowly charged. Immunofluorescence localization of trout CAMPs demonstrated that these peptides expressed mainly at the mucosal layer of gut. Meanwhile, signals around sinusoids were also detected in head kidney. Moreover, the biological activities of trout CAMPs were proved to be mediated by the N terminus. Additionally, the repetitive motifs characteristically existing in Salmonidae increased the structural flexibilities of peptides and further increased the antibacterial and IL-8-stimulating activities. Unlike most a helical and cytotoxic mammalian CAMPs, trout CAMPs, mainly consisting of beta-sheet and random coil, exhibited no cytotoxic activities. The distinctive structural features of trout CAMPs provide new insights into the understanding of the evolution of CAMPs in vertebrates. Moreover, the high bacterial membrane selectivity of trout CAMPs will help to design excellent peptide antibiotics.
SubtypeArticle
Department[Zhang, Xu-Jie ; Zhang, Xiang-Yang ; Zhang, Nu ; Guo, Xia ; Peng, Kai-Song ; Wu, Han ; Lu, Long-Feng ; Wu, Nan ; Chen, Dan-Dan ; Li, Shun ; Nie, Pin ; Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Zhang, Xu-Jie ; Zhang, Xiang-Yang ; Zhang, Nu ; Guo, Xia ; Lu, Long-Feng ; Chen, Dan-Dan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Peng, Kai-Song] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China
DOI10.4049/jimmunol.1500182
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology
WOS SubjectImmunology
WOS KeywordSECONDARY STRUCTURE ANALYSES ; FUNCTIONAL-CHARACTERIZATION ; ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; HOST-DEFENSE ; GENE FAMILY ; LL-37 ; EXPRESSION ; INNATE ; IDENTIFICATION
WOS IDWOS:000353728800039
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23978
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorZhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xu-Jie,Zhang, Xiang-Yang,Zhang, Nu,et al. Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish[J]. JOURNAL OF IMMUNOLOGY,2015,194(10):4974-4987.
APA Zhang, Xu-Jie.,Zhang, Xiang-Yang.,Zhang, Nu.,Guo, Xia.,Peng, Kai-Song.,...&Zhang, YA .(2015).Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish.JOURNAL OF IMMUNOLOGY,194(10),4974-4987.
MLA Zhang, Xu-Jie,et al."Distinctive Structural Hallmarks and Biological Activities of the Multiple Cathelicidin Antimicrobial Peptides in a Primitive Teleost Fish".JOURNAL OF IMMUNOLOGY 194.10(2015):4974-4987.
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