Dietary leucine regulates the intestinal immune status, immune-related signalling molecules and tight junction transcript abundance in grass carp (Ctenopharyngodon idella)
Jiang, Wei-Dan1,2,3; Deng, Yu-Ping1; Liu, Yang1,2,3; Qu, Biao1; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Wu, Pei1; Zhang, Yong-An5; Zhou, Xiao-Qiu1,2,3; Feng, Lin1,2,3; Zhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.
2015-07-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume444Pages:134-142
AbstractThe role of leucine (Leu) in the regulation of the intestinal immune status, immune-related signalling molecules and tight junction (TJ) protein transcript abundance in the intestine of grass carp ( Ctenopharyngodon idella) was investigated. Six iso-nitrogenous diets that contained graded levels of Leu (7.1-17.1 g Leu kg(-1) diets) were fed to the fish for 8 weeks. Compared with the control group, appropriate Leu supplementation increased (P < 0.05) the following: (1) the lysozyme activity, acid phosphatase activity and complement component 3 (C3) content in all intestinal segments; (2) the mRNA levels of interleukin 10, inhibitor factor kappa B alpha (I kappa Ba) and target of rapamycin ( TOR) in the mid and distal intestine as well as transforming growth factor beta 1 in all intestinal segments; and ( 3) the transcript levels for claudin b, claudin 3, claudin 15, occludin and zonula occludens-1 (ZO-1) in the intestine of young grass carp. At the same time, appropriate Leu supplementation down-regulated the mRNA levels of tumour necrosis factor a, interleukin 8 and nuclear factor kappa B p65 ( NF-kappa B p65) in the mid and distal intestine of young grass carp (P < 0.05). Interestingly, the transcript levels for claudin c and claudin 12 showed no significant differences among the groups in the intestine of young grass carp. In conclusion, the positive effect of Leu on intestinal health is associated with the improvement of the intestinal immune status and the regulation of immune-related signalling molecules and tight junction transcripts of fish. (C) 2015 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordLeucine Intestinal Immune Tight Junction Nuclear Factor Kappa b P65 Target Of Rapamycin
Department[Jiang, Wei-Dan ; Deng, Yu-Ping ; Liu, Yang ; Qu, Biao ; Jiang, Jun ; Wu, Pei ; Zhou, Xiao-Qiu ; Feng, Lin] Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China ; [Jiang, Wei-Dan ; Liu, Yang ; Jiang, Jun ; Zhou, Xiao-Qiu ; Feng, Lin] Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China ; [Jiang, Wei-Dan ; Liu, Yang ; Jiang, Jun ; Zhou, Xiao-Qiu ; Feng, Lin] Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr China, Minist Educ, Chengdu 611130, Sichuan, Peoples R China ; [Kuang, Sheng-Yao ; Tang, Ling ; Tang, Wu-Neng] Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China ; [Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1016/j.aquaculture.2015.04.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS KeywordNF-KAPPA-B ; CHAIN AMINO-ACIDS ; GENE-EXPRESSION ; TNF-ALPHA ; ANTIOXIDANT STATUS ; GROWTH-PERFORMANCE ; DISEASE RESISTANCE ; BARRIER FUNCTION ; INNATE IMMUNITY ; JUVENILE JIAN
WOS IDWOS:000353644800018
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23977
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorZhou, XQ (reprint author), Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China.
Affiliation1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China
3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr China, Minist Educ, Chengdu 611130, Sichuan, Peoples R China
4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Wei-Dan,Deng, Yu-Ping,Liu, Yang,et al. Dietary leucine regulates the intestinal immune status, immune-related signalling molecules and tight junction transcript abundance in grass carp (Ctenopharyngodon idella)[J]. AQUACULTURE,2015,444:134-142.
APA Jiang, Wei-Dan.,Deng, Yu-Ping.,Liu, Yang.,Qu, Biao.,Jiang, Jun.,...&Zhou, XQ .(2015).Dietary leucine regulates the intestinal immune status, immune-related signalling molecules and tight junction transcript abundance in grass carp (Ctenopharyngodon idella).AQUACULTURE,444,134-142.
MLA Jiang, Wei-Dan,et al."Dietary leucine regulates the intestinal immune status, immune-related signalling molecules and tight junction transcript abundance in grass carp (Ctenopharyngodon idella)".AQUACULTURE 444(2015):134-142.
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