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Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells
Zheng, Peng1; Xiong, Qian1; Wu, Ying1; Chen, Ying1; Chen, Zhuo1; Fleming, Joy2; Gao, Ding3; Bi, Lijun2; Ge, Feng1; Ge, F (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2015-06-01
Source PublicationMOLECULAR & CELLULAR PROTEOMICS
ISSN1535-9476
Volume14Issue:6Pages:1447-1463
AbstractLong noncoding RNAs (IncRNAs), which have emerged in recent years as a new and crucial layer of gene regulators, regulate various biological processes such as carcinogenesis and metastasis. HOTAIR (Hox transcript antisense intergenic RNA), a lncRNA overexpressed in most human cancers, has been shown to be an oncogenic lncRNA. Here, we explored the role of HOTAIR in HeLa cells and searched for proteins regulated by HOTAIR. To understand the mechanism of action of HOTAIR from a systems perspective, we employed a quantitative proteomic strategy to systematically identify potential targets of HOTAIR. The expression of 170 proteins was significantly dys-regulated after inhibition of HOTAIR, implying that they could be potential targets of HOTAIR. Analysis of this data at the systems level revealed major changes in proteins involved in diverse cellular components, including the cytoskeleton and the respiratory chain. Further functional studies on vimentin (VIM), a key protein involved in the cytoskeleton, revealed that HOTAIR exerts its effects on migration and invasion of HeLa cells, at least in part, through the regulation of VIM expression. Inhibition of HOTAIR leads to mitochondrial dysfunction and ultrastructural alterations, suggesting a novel role of HOTAIR in maintaining mitochondrial function in cancer cells. Our results provide novel insights into the mechanisms underlying the function of HOTAIR in cancer cells. We expect that the methods used in this study will become an integral part of functional studies of IncRNAs.
SubtypeArticle
Department[Zheng, Peng ; Xiong, Qian ; Wu, Ying ; Chen, Ying ; Chen, Zhuo ; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Fleming, Joy ; Bi, Lijun] Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China ; [Gao, Ding] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430072, Peoples R China
DOI10.1074/mcp.M114.043984
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000355550400002
WOS KeywordVIMENTIN INTERMEDIATE-FILAMENTS ; LARGE GENE LISTS ; EXPERIMENTAL COEXPRESSION ; INTERACTION NETWORKS ; CANCER METASTASIS ; BREAST-CANCER ; UP-REGULATION ; IN-VIVO ; EXPRESSION ; CARCINOMA
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23972
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorGe, F (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Peng,Xiong, Qian,Wu, Ying,et al. Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells[J]. MOLECULAR & CELLULAR PROTEOMICS,2015,14(6):1447-1463.
APA Zheng, Peng.,Xiong, Qian.,Wu, Ying.,Chen, Ying.,Chen, Zhuo.,...&Ge, F .(2015).Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells.MOLECULAR & CELLULAR PROTEOMICS,14(6),1447-1463.
MLA Zheng, Peng,et al."Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells".MOLECULAR & CELLULAR PROTEOMICS 14.6(2015):1447-1463.
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