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题名: Quantitative Proteomics Analysis Reveals Novel Insights into Mechanisms of Action of Long Noncoding RNA Hox Transcript Antisense Intergenic RNA (HOTAIR) in HeLa Cells
作者: Zheng, Peng1; Xiong, Qian1; Wu, Ying1; Chen, Ying1; Chen, Zhuo1; Fleming, Joy2; Gao, Ding3; Bi, Lijun2; Ge, Feng1
通讯作者: Ge, F (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
刊名: MOLECULAR & CELLULAR PROTEOMICS
发表日期: 2015-06-01
DOI: 10.1074/mcp.M114.043984
卷: 14, 期:6, 页:1447-1463
收录类别: SCI
文章类型: Article
部门归属: [Zheng, Peng ; Xiong, Qian ; Wu, Ying ; Chen, Ying ; Chen, Zhuo ; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Fleming, Joy ; Bi, Lijun] Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China ; [Gao, Ding] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Long noncoding RNAs (IncRNAs), which have emerged in recent years as a new and crucial layer of gene regulators, regulate various biological processes such as carcinogenesis and metastasis. HOTAIR (Hox transcript antisense intergenic RNA), a lncRNA overexpressed in most human cancers, has been shown to be an oncogenic lncRNA. Here, we explored the role of HOTAIR in HeLa cells and searched for proteins regulated by HOTAIR. To understand the mechanism of action of HOTAIR from a systems perspective, we employed a quantitative proteomic strategy to systematically identify potential targets of HOTAIR. The expression of 170 proteins was significantly dys-regulated after inhibition of HOTAIR, implying that they could be potential targets of HOTAIR. Analysis of this data at the systems level revealed major changes in proteins involved in diverse cellular components, including the cytoskeleton and the respiratory chain. Further functional studies on vimentin (VIM), a key protein involved in the cytoskeleton, revealed that HOTAIR exerts its effects on migration and invasion of HeLa cells, at least in part, through the regulation of VIM expression. Inhibition of HOTAIR leads to mitochondrial dysfunction and ultrastructural alterations, suggesting a novel role of HOTAIR in maintaining mitochondrial function in cancer cells. Our results provide novel insights into the mechanisms underlying the function of HOTAIR in cancer cells. We expect that the methods used in this study will become an integral part of functional studies of IncRNAs.
关键词[WOS]: VIMENTIN INTERMEDIATE-FILAMENTS ; LARGE GENE LISTS ; EXPERIMENTAL COEXPRESSION ; INTERACTION NETWORKS ; CANCER METASTASIS ; BREAST-CANCER ; UP-REGULATION ; IN-VIVO ; EXPRESSION ; CARCINOMA
语种: 英语
WOS记录号: WOS:000355550400002
ISSN号: 1535-9476
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23972
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430072, Peoples R China
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