IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Structural organization of an intact phycobilisome and its association with photosystem II
Chang, Leifu1,2; Liu, Xianwei3; Li, Yanbing3; Liu, Cui-Cui1,2; Yang, Fan1,2; Zhao, Jindong3,4; Sui, Sen-Fang1,2; Zhao, JD (reprint author), Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China.
2015-06-01
Source PublicationCELL RESEARCH
ISSN1001-0602
Volume25Issue:6Pages:726-737
AbstractPhycobilisomes (PBSs) are light-harvesting antennae that transfer energy to photosynthetic reaction centers in cyanobacteria and red algae. PBSs are supermolecular complexes composed of phycobiliproteins (PBPs) that bear chromophores for energy absorption and linker proteins. Although the structures of some individual components have been determined using crystallography, the three-dimensional structure of an entire PBS complex, which is critical for understanding the energy transfer mechanism, remains unknown. Here, we report the structures of an intact PBS and a PBS in complex with photosystem II (PSII) from Anabaena sp. strain PCC 7120 using single-particle electron microscopy in combination with biochemical and molecular analyses. In the PBS structure, all PBP trimers and the conserved linker protein domains were unambiguously located, and the global distribution of all chromophores was determined. We provide evidence that ApcE and ApcF are critical for the formation of a protrusion at the bottom of PBS, which plays an important role in mediating PBS interaction with PSII. Our results provide insights into the molecular architecture of an intact PBS at different assembly levels and provide the basis for understanding how the light energy absorbed by PBS is transferred to PSII.
SubtypeArticle
KeywordPhycobilisome Photosynthesis Phycobiliprotein Electron Microscopy
Department[Chang, Leifu ; Liu, Cui-Cui ; Yang, Fan ; Sui, Sen-Fang] Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China ; [Chang, Leifu ; Liu, Cui-Cui ; Yang, Fan ; Sui, Sen-Fang] Tsinghua Univ, Sch Life Sci, Ctr Struct Biol, Beijing 100084, Peoples R China ; [Liu, Xianwei ; Li, Yanbing ; Zhao, Jindong] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China ; [Zhao, Jindong] Chinese Acad Sci, Inst Hydrobiol, Key Lab Phycol, Wuhan 430072, Hubei, Peoples R China
DOI10.1038/cr.2015.59
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000355554800009
WOS KeywordALLOPHYCOCYANIN CORE COMPLEX ; CRYSTAL-STRUCTURE ; CYANOBACTERIAL PHYCOBILISOMES ; ELECTRON-MICROSCOPY ; C-PHYCOCYANIN ; MASTIGOCLADUS-LAMINOSUS ; PORPHYRIDIUM-CRUENTUM ; ENERGY-TRANSFER ; PCC 7120 ; RESOLUTION
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23971
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhao, JD (reprint author), Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China.
Affiliation1.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
2.Tsinghua Univ, Sch Life Sci, Ctr Struct Biol, Beijing 100084, Peoples R China
3.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Phycol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Chang, Leifu,Liu, Xianwei,Li, Yanbing,et al. Structural organization of an intact phycobilisome and its association with photosystem II[J]. CELL RESEARCH,2015,25(6):726-737.
APA Chang, Leifu.,Liu, Xianwei.,Li, Yanbing.,Liu, Cui-Cui.,Yang, Fan.,...&Zhao, JD .(2015).Structural organization of an intact phycobilisome and its association with photosystem II.CELL RESEARCH,25(6),726-737.
MLA Chang, Leifu,et al."Structural organization of an intact phycobilisome and its association with photosystem II".CELL RESEARCH 25.6(2015):726-737.
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