中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Repression of hypoxia-inducible factor alpha signaling by Set7-mediated methylation
作者: Liu, Xing1, 2; Chen, Zhu2, 3; Xu, Chenxi1, 2; Leng, Xiaoqian1, 2; Cao, Hong1, 2; Ouyang, Gang2; Xiao, Wuhan1, 2
通讯作者: Xiao, WH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2015-05-26
DOI: 10.1093/nar/gkv379
卷: 43, 期:10, 页:5081-5098
收录类别: SCI
文章类型: Article
部门归属: [Liu, Xing ; Xu, Chenxi ; Leng, Xiaoqian ; Cao, Hong ; Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Liu, Xing ; Chen, Zhu ; Xu, Chenxi ; Leng, Xiaoqian ; Cao, Hong ; Ouyang, Gang ; Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Chen, Zhu] Maternal & Child Hlth Hosp Hubei Prov, Dept Reprod, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Hypoxia-inducible factor (HIF)-1 alpha and HIF-2 alpha are the main regulators of cellular responses to hypoxia. Post-translational modifications of HIF-1 alpha and 2 alpha are necessary to modulate their functions. The methylation of non-histone proteins by Set7, an SET domain-containing lysine methyltransferase, is a novel regulatory mechanism to control cell protein function in response to various cellular stresses. In this study, we show that Set7 methylates HIF-1 alpha at lysine 32 and HIF-2 alpha at lysine K29; this methylation inhibits the expression of HIF-1 alpha/2 alpha targets by impairing the occupancy of HIF-alpha on hypoxia response element of HIF target gene promoter. Set7-null fibroblasts and the cells with shRNA-knocked down Set7 exhibit upregulated HIF target genes. Set7 inhibitor blocks HIF-1 alpha/2 alpha methylation to enhance HIF target gene expression. Set7-null fibroblasts and the cells with shRNA-knocked down Set7 or inhibition of Set7 by the inhibitor subjected to hypoxia display an increased glucose uptake and intracellular adenosine triphosphate levels. These findings define a novel modification of HIF-1 alpha/2 alpha and demonstrate that Set7-medited lysine methylation negatively regulates HIF-alpha transcriptional activity and HIF-1 alpha-mediated glucose homeostasis.
关键词[WOS]: TUMOR-SUPPRESSOR PROTEIN ; INDUCED CELL-DEATH ; LYSINE METHYLATION ; TRANSCRIPTIONAL ACTIVITY ; SET9-MEDIATED METHYLATION ; PHOSPHORYLATION SWITCH ; STABILIZES HIF-1-ALPHA ; HISTONE DEMETHYLASES ; NONHISTONE PROTEINS ; GLUCOSE-METABOLISM
语种: 英语
WOS记录号: WOS:000355929200029
ISSN号: 0305-1048
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23970
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Repression of hypoxia-inducible factor alpha signaling by Set7-mediated methylation.pdf(10267KB)----开放获取View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
3.Maternal & Child Hlth Hosp Hubei Prov, Dept Reprod, Wuhan, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Xing]'s Articles
[Chen, Zhu]'s Articles
[Xu, Chenxi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Xing]‘s Articles
[Chen, Zhu]‘s Articles
[Xu, Chenxi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Repression of hypoxia-inducible factor alpha signaling by Set7-mediated methylation.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace