Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4
Li, Shun1; Guo, Xia1,2; Lu, Long-Feng1,2; Lu, Xiao-Bing1,2; Wu, Nan1; Zhang, Yong-An1; Zhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2015-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume51Issue:1Pages:65-73
AbstractInterferon regulatory factor 4 (IRF4) plays pivotal roles in both innate and adaptive immune responses in mammals. In fish, there are two homologues of irf4, irf4a and irf4b. In this study, we examined the regulatory patterns of zebrafish irf4a and irf4b by STAT6 and c-Rel. Firstly, expression of irf4a and irf4b was monitored in several tissues at mRNA level. By infection with SVCV, irf4a and irf4b were upregulated in both kidney and spleen, and were immediately induced by treatment with poly I:C in ZF4 cells. Moreover, the activation of irf4a promoter was regulated by overexpression of stat6 and c-rel in a cooperation manner, which could be inhibited by mutation of the putative binding sites of STAT6 and c-Rel in irf4a promoter region. However, irf4b promoter was activated slightly only by STAT6 but not c-Rel. Furthermore, overexpression of irf4a inhibited the activation of its own promoter under induction of STAT6 and c-Rel, which was the result of that IRF4a bound to STAT6 and c-Rel directly. In addition, cellular location analysis showed that IRF4a was located only in nuclear region. These data indicate that fish irf4a can also be upregulated by STAT6 and c-Rel. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordIrf4 Interferon Regulatory Factor Stat6 C-rel Zebrafish Svcv
Department[Li, Shun ; Guo, Xia ; Lu, Long-Feng ; Lu, Xiao-Bing ; Wu, Nan ; Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Guo, Xia ; Lu, Long-Feng ; Lu, Xiao-Bing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1016/j.dci.2015.02.018
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS KeywordB-CELL DEVELOPMENT ; NF-KAPPA-B ; TRANSCRIPTION FACTOR ; INTERLEUKIN-4-INDUCED TRANSCRIPTION ; FACTOR FAMILY ; MATURE B ; EXPRESSION ; ACTIVATION ; VIRUSES ; DIFFERENTIATION
WOS IDWOS:000357906800008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23967
Collection藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心
Corresponding AuthorZhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Shun,Guo, Xia,Lu, Long-Feng,et al. Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):65-73.
APA Li, Shun.,Guo, Xia.,Lu, Long-Feng.,Lu, Xiao-Bing.,Wu, Nan.,...&Zhang, YA .(2015).Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),65-73.
MLA Li, Shun,et al."Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):65-73.
Files in This Item:
File Name/Size DocType Version Access License
Regulation pattern o(1675KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li, Shun]'s Articles
[Guo, Xia]'s Articles
[Lu, Long-Feng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li, Shun]'s Articles
[Guo, Xia]'s Articles
[Lu, Long-Feng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li, Shun]'s Articles
[Guo, Xia]'s Articles
[Lu, Long-Feng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.