| Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4 | |
| Li, Shun1; Guo, Xia1,2; Lu, Long-Feng1,2; Lu, Xiao-Bing1,2; Wu, Nan1; Zhang, Yong-An1; Zhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. | |
| 2015-07-01 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| ISSN | 0145-305X |
| Volume | 51Issue:1Pages:65-73 |
| Abstract | Interferon regulatory factor 4 (IRF4) plays pivotal roles in both innate and adaptive immune responses in mammals. In fish, there are two homologues of irf4, irf4a and irf4b. In this study, we examined the regulatory patterns of zebrafish irf4a and irf4b by STAT6 and c-Rel. Firstly, expression of irf4a and irf4b was monitored in several tissues at mRNA level. By infection with SVCV, irf4a and irf4b were upregulated in both kidney and spleen, and were immediately induced by treatment with poly I:C in ZF4 cells. Moreover, the activation of irf4a promoter was regulated by overexpression of stat6 and c-rel in a cooperation manner, which could be inhibited by mutation of the putative binding sites of STAT6 and c-Rel in irf4a promoter region. However, irf4b promoter was activated slightly only by STAT6 but not c-Rel. Furthermore, overexpression of irf4a inhibited the activation of its own promoter under induction of STAT6 and c-Rel, which was the result of that IRF4a bound to STAT6 and c-Rel directly. In addition, cellular location analysis showed that IRF4a was located only in nuclear region. These data indicate that fish irf4a can also be upregulated by STAT6 and c-Rel. (C) 2015 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Irf4 Interferon Regulatory Factor Stat6 C-rel Zebrafish Svcv |
| Department | [Li, Shun ; Guo, Xia ; Lu, Long-Feng ; Lu, Xiao-Bing ; Wu, Nan ; Zhang, Yong-An] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Guo, Xia ; Lu, Long-Feng ; Lu, Xiao-Bing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| DOI | 10.1016/j.dci.2015.02.018 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Immunology ; Zoology |
| WOS Subject | Immunology ; Zoology |
| WOS Keyword | B-CELL DEVELOPMENT ; NF-KAPPA-B ; TRANSCRIPTION FACTOR ; INTERLEUKIN-4-INDUCED TRANSCRIPTION ; FACTOR FAMILY ; MATURE B ; EXPRESSION ; ACTIVATION ; VIRUSES ; DIFFERENTIATION |
| WOS ID | WOS:000357906800008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/23967 |
| Collection | 藻类生物学及应用研究中心_水生生物分子与细胞生物学研究中心 |
| Corresponding Author | Zhang, YA (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Shun,Guo, Xia,Lu, Long-Feng,et al. Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):65-73. |
| APA | Li, Shun.,Guo, Xia.,Lu, Long-Feng.,Lu, Xiao-Bing.,Wu, Nan.,...&Zhang, YA .(2015).Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),65-73. |
| MLA | Li, Shun,et al."Regulation pattern of fish irf4 (the gene encoding IFN regulatory factor 4) by STAT6, c-Rel and IRF4".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):65-73. |
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