中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水环境工程研究中心  > 期刊论文
题名: Perfluorooctyl Iodide Stimulates Steroidogenesis in H295R Cells via a Cyclic Adenosine Monophosphate Signaling Pathway
作者: Wang, Chang1, 2; Ruan, Ting2; Liu, Jiyan2; He, Bin2; Zhou, Qunfang2; Jiang, Guibin2
通讯作者: Jiang, GB (reprint author), Chinese Acad Sci, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China.
刊名: CHEMICAL RESEARCH IN TOXICOLOGY
发表日期: 2015-05-01
DOI: 10.1021/tx5004563
卷: 28, 期:5, 页:848-854
收录类别: SCI
文章类型: Article
部门归属: [Wang, Chang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Wang, Chang ; Ruan, Ting ; Liu, Jiyan ; He, Bin ; Zhou, Qunfang ; Jiang, Guibin] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry ; Toxicology
英文摘要: Perfluorinated iodine alkalies (PFIs) are used widely in the organic fluorine industry. Increased production of PFIs has caused environmental health concern. To evaluate the potential endocrine-disrupting effect of PFIs, we investigated the effects of perfluorooctyl iodide (PFOI) on steroidogenesis in human adrenocortical carcinoma cells (H295R). Levels of aldosterone, 17 beta-estradiol, and testosterone,were measured in H295R culture medium upon treatment with perfluorooctanoic acid (PFOA) and PFIs. Expression of 10 steroidogenic genes (StAR, HMGR, CYP11A1 3 beta HSD2, 17 beta HSD; CYP17, CYP21, CYP11B1, CYP11B2, and CYP19) was measured by-real-time polymerase chain reaction. Levels of cyclic adenosine monophosphate (cAMP) and adenylate cyclase (AC) activity were measured to understand the underlying mechanism of steroidogenic perturbations. Levels of production of aldosterone, cortisol, and 17 beta-estradiol were elevated significantly, and the level of testosterone generation decreased upon treatment with 100 mu M PFOI. Similar to the, effect induced by forskolin (AC activator), expression of all 10 genes involved in the synthesis of steroid hormones was upregulated significantly upon exposure to 100 mu M PFOI. PFOA had no effect On steroid hormone production or steroidogenic gene expression even though it is highly structurally similar with PFOI. Therefore, the terminal -CF2I roup in PFOI could-be a critical factor for mediation of steroidogenesis. PFOI increased AC activity and cAMP levels in H295R cells, which implied an underlying mechanism for the disturbance of steroidogenesis. These data suggest that PFOI may act as an AC activator,, thereby stimulating steroidogenesis by activating a cAMP signaling pathway.
关键词[WOS]: PERFLUORINATED CARBOXYLIC-ACIDS ; STEROID-HORMONE BIOSYNTHESIS ; ACUTE REGULATORY PROTEIN ; MEDAKA ORYZIAS-LATIPES ; IN-VITRO ; FLUOROTELOMER ALCOHOLS ; VITELLOGENIN INDUCTION ; ESTROGENIC ACTIVITIES ; HEPATIC VITELLOGENIN ; PBDE METABOLITES
语种: 英语
WOS记录号: WOS:000354907500003
ISSN号: 0893-228X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23964
Appears in Collections:水环境工程研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Perfluorooctyl Iodide Stimulates Steroidogenesis in H295R Cells via a Cyclic Adenosine Monophosphate Signaling Pathway.pdf(875KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Chang]'s Articles
[Ruan, Ting]'s Articles
[Liu, Jiyan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Chang]‘s Articles
[Ruan, Ting]‘s Articles
[Liu, Jiyan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Perfluorooctyl Iodide Stimulates Steroidogenesis in H295R Cells via a Cyclic Adenosine Monophosphate Signaling Pathway.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace