| Developmental exposure to the organophosphorus flame retardant tris(1,3-dichloro-2-propyl) phosphate: Estrogenic activity, endocrine disruption and reproductive effects on zebrafish | |
| Wang, Qiangwei1,2; Lam, James C. W.3; Han, Jian3; Wang, Xianfeng1; Guo, Yongyong1; Lam, Paul K. S.3; Zhou, Bingsheng1; Zhou, BS (reprint author), Chinese Acad Sci, Zhou Inst Hydrobiol, Wuhan 430072, Peoples R China. | |
| 2015-03-01 | |
| Source Publication | AQUATIC TOXICOLOGY
 |
| ISSN | 0166-445X |
| Volume | 160Pages:163-171 |
| Abstract | Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is an organophosphate flame retardant that is detectable in the environment and biota, prompting concern over its risk to wildlife and human health. Our objective was to investigate whether long-term exposure to low concentrations of TDCPP can affect fish reproduction. Zebrafish embryos were exposed to low concentrations (0, 4, 20 and 100 mu g/L) of TDCPP from 2 h post-fertilization until sexual maturation. Exposure to TDCPP significantly increased plasma estradiol and testosterone levels in females, but had no effect in males. TDCPP exposure also caused a significant reduction in fecundity as indicated by decreased egg production. Real-time PCR was performed to examine selected genes in the hypothalamic-pituitary-gonadal (HPG) axis and liver. Principle component analysis (PCA) showed that sex hormone levels and fecundity were related to the mRNA level of several genes in the HPG axis. Furthermore, hepatic vitellogenin (vtg1 and vtg3) expression was upregulated in both females and males, suggesting TDCPP has estrogenic activity. Histological examination revealed promotion of oocyte maturation in the females, but retardation of spermiation in males. Reduced egg quality (e.g., egg diameter) and increased malformation rates were observed in the F1 generation. Chemical analysis showed significant levels of TDCPP and its metabolite bis(1,3-dichloro-2-propyl) phosphate in the gonads of males and females. In conclusion, long-term exposure to low concentrations of TDCPP impairs fish reproduction. (C) 2015 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Organophosphate Flame Retardant Tdcpp Bioconcentration Steroid Hormone Endocrine Disruption Reproduction Zebrafish |
| Department | [Wang, Qiangwei ; Wang, Xianfeng ; Guo, Yongyong ; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Wang, Qiangwei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Lam, James C. W. ; Han, Jian ; Lam, Paul K. S.] City Univ Hong Kong, Dept Biol & Chem, State Key Lab Marine Pollut, Hong Kong, Hong Kong, Peoples R China |
| DOI | 10.1016/j.aquatox.2015.01.014 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Marine & Freshwater Biology ; Toxicology |
| WOS Subject | Marine & Freshwater Biology ; Toxicology |
| WOS ID | WOS:000350941000016 |
| WOS Keyword | MESSENGER-RNA EXPRESSION ; DIPHENYL ETHERS PBDES ; DANIO-RERIO ; GENE-EXPRESSION ; GONADOTROPIN RECEPTORS ; PIMEPHALES-PROMELAS ; SEX-DIFFERENTIATION ; MASS-SPECTROMETRY ; TREATMENT-PLANT ; TDCPP |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/23949 |
| Collection | 水环境工程研究中心_期刊论文 |
| Corresponding Author | Zhou, BS (reprint author), Chinese Acad Sci, Zhou Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.City Univ Hong Kong, Dept Biol & Chem, State Key Lab Marine Pollut, Hong Kong, Hong Kong, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Qiangwei,Lam, James C. W.,Han, Jian,et al. Developmental exposure to the organophosphorus flame retardant tris(1,3-dichloro-2-propyl) phosphate: Estrogenic activity, endocrine disruption and reproductive effects on zebrafish[J]. AQUATIC TOXICOLOGY,2015,160:163-171. |
| APA | Wang, Qiangwei.,Lam, James C. W..,Han, Jian.,Wang, Xianfeng.,Guo, Yongyong.,...&Zhou, BS .(2015).Developmental exposure to the organophosphorus flame retardant tris(1,3-dichloro-2-propyl) phosphate: Estrogenic activity, endocrine disruption and reproductive effects on zebrafish.AQUATIC TOXICOLOGY,160,163-171. |
| MLA | Wang, Qiangwei,et al."Developmental exposure to the organophosphorus flame retardant tris(1,3-dichloro-2-propyl) phosphate: Estrogenic activity, endocrine disruption and reproductive effects on zebrafish".AQUATIC TOXICOLOGY 160(2015):163-171. |
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