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A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM
Wang, Chunbo1,2; Wu, Xingqiang1; Tian, Cuicui1,2; Li, Qian1,2; Tian, Yingying1,2; Feng, Bing1,2; Xiao, Bangding1; Xiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2015-04-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume27Issue:2Pages:711-720
AbstractColony formation plays an important role in the life history of Microcystis. However, analyzing the colony size distribution with a microscope is time consuming, and colony formation also hinders the direct monitoring of cell density. In this study, a quantitative protocol for rapidly analyzing the cell density and colony size distribution of pelagic and benthic Microcystis was developed. Microcystis colonies were disintegrated by alkaline hydrolysis with 0.01-0.05 mol L-1 sodium hydroxide at 85 A degrees C for 6-8 min and automatically measured by the Flow Camera And Microscope (FlowCAM). Benthic Microcystis colonies were isolated from different lake sediments using 40 % Percoll solution. Alkaline hydrolysis was validated as a rapid and universally effective method to disintegrate different morphospecies of Microcystis colonies. The FlowCAM exhibited excellent accuracy, reproducibility, and efficiency in determining the cell density and size distribution of Microcystis. The developed protocol was successfully applied to field samples from Lake Caohai (China) and can accurately monitor the population dynamics and colony size distribution of bloom-forming Microcystis.
SubtypeArticle
KeywordMicrocystis Cell Density Size Distribution Flowcam Aalkaline Hydrolysis
Department[Wang, Chunbo ; Wu, Xingqiang ; Tian, Cuicui ; Li, Qian ; Tian, Yingying ; Feng, Bing ; Xiao, Bangding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Wang, Chunbo ; Tian, Cuicui ; Li, Qian ; Tian, Yingying ; Feng, Bing] Univ Chinese Acad Sci, Beijing 10085, Peoples R China
DOI10.1007/s10811-014-0352-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS KeywordBLOOM-FORMING MICROCYSTIS ; CYANOBACTERIUM MICROCYSTIS ; LAKE-ERIE ; AERUGINOSA ; CHINA ; PHYTOPLANKTON ; ENUMERATION ; DIVERSITY ; TAIHU ; CYANOPHYCEAE
WOS IDWOS:000352446200009
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23944
Collection水环境工程研究中心
Corresponding AuthorXiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10085, Peoples R China
Recommended Citation
GB/T 7714
Wang, Chunbo,Wu, Xingqiang,Tian, Cuicui,et al. A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM[J]. JOURNAL OF APPLIED PHYCOLOGY,2015,27(2):711-720.
APA Wang, Chunbo.,Wu, Xingqiang.,Tian, Cuicui.,Li, Qian.,Tian, Yingying.,...&Xiao, BD .(2015).A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM.JOURNAL OF APPLIED PHYCOLOGY,27(2),711-720.
MLA Wang, Chunbo,et al."A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM".JOURNAL OF APPLIED PHYCOLOGY 27.2(2015):711-720.
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