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题名: A quantitative protocol for rapid analysis of cell density and size distribution of pelagic and benthic Microcystis colonies by FlowCAM
作者: Wang, Chunbo1, 2; Wu, Xingqiang1; Tian, Cuicui1, 2; Li, Qian1, 2; Tian, Yingying1, 2; Feng, Bing1, 2; Xiao, Bangding1
通讯作者: Xiao, BD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
关键词: Microcystis ; Cell density ; Size distribution ; FlowCAM ; Aalkaline hydrolysis
刊名: JOURNAL OF APPLIED PHYCOLOGY
发表日期: 2015-04-01
DOI: 10.1007/s10811-014-0352-0
卷: 27, 期:2, 页:711-720
收录类别: SCI
文章类型: Article
部门归属: [Wang, Chunbo ; Wu, Xingqiang ; Tian, Cuicui ; Li, Qian ; Tian, Yingying ; Feng, Bing ; Xiao, Bangding] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Wang, Chunbo ; Tian, Cuicui ; Li, Qian ; Tian, Yingying ; Feng, Bing] Univ Chinese Acad Sci, Beijing 10085, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
英文摘要: Colony formation plays an important role in the life history of Microcystis. However, analyzing the colony size distribution with a microscope is time consuming, and colony formation also hinders the direct monitoring of cell density. In this study, a quantitative protocol for rapidly analyzing the cell density and colony size distribution of pelagic and benthic Microcystis was developed. Microcystis colonies were disintegrated by alkaline hydrolysis with 0.01-0.05 mol L-1 sodium hydroxide at 85 A degrees C for 6-8 min and automatically measured by the Flow Camera And Microscope (FlowCAM). Benthic Microcystis colonies were isolated from different lake sediments using 40 % Percoll solution. Alkaline hydrolysis was validated as a rapid and universally effective method to disintegrate different morphospecies of Microcystis colonies. The FlowCAM exhibited excellent accuracy, reproducibility, and efficiency in determining the cell density and size distribution of Microcystis. The developed protocol was successfully applied to field samples from Lake Caohai (China) and can accurately monitor the population dynamics and colony size distribution of bloom-forming Microcystis.
关键词[WOS]: BLOOM-FORMING MICROCYSTIS ; CYANOBACTERIUM MICROCYSTIS ; LAKE-ERIE ; AERUGINOSA ; CHINA ; PHYTOPLANKTON ; ENUMERATION ; DIVERSITY ; TAIHU ; CYANOPHYCEAE
语种: 英语
WOS记录号: WOS:000352446200009
ISSN号: 0921-8971
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23944
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 10085, Peoples R China
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