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Chloro-benzoquinones cause oxidative DNA damage through iron-mediated ROS production in Escherichia coli
Chen, Zhilan1,2; Zhou, Qiaohong1; Zou, Dandan2,3; Tian, Yun1,3; Liu, Biyun1; Zhang, Yongyuan1; Wu, Zhenbin1; Zhou, QH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
2015-09-01
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume135Pages:379-386
AbstractChloro-benzoquinones (CBQs) are a group of disinfection byproducts that are suspected to be potentially carcinogenic. Here, the mechanism of DNA damage caused by CBQs in the presence of ferrous ions was investigated in an Escherichia coil wild type M5 strain and a mutant L5 (ahpCF katEG mutant) strain that carried an enhanced green fluorescent protein reporter under the control of a SOS response gene (recA) promoter. All tested CBQs (including para-benzoquinone, 2-chloro-para-benzoquinone, and dichloro-para-benzoquinones with different substitutes) caused substantial oxidative DNA damage with EC50 values in the micromolar range. Moreover, 2,5-dichloro-para-benzoquinone (2,5-DCBQ), a typical CBQ caused substantial ROS production in Escherichia coil mutant cells. And ROS scavengers provided partial protective effects on genotoxicity of 2,5-DCBQ to Escherichia coil mutant cells. The addition of Fe2+ to the 2,5-DCBQ exposure system caused an increase in DNA oxidative damage; iron-chelating agents could partially prevent these cells from DNA damage. Finally, intracellular AhpCF, catalase E, and catalase G were all found to play an important role in the survival of E. coil cells exposed to CBQs, as indicated by an increased sensitivity of the ahpCF katEG mutant L5 strain to treatment compared with wild type M5 cells. Taken together, these results suggest that CBQs cause oxidative DNA damage in Escherichia coil cells through the participation of iron-mediated ROS production. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordChloro-benzoquinones Oxidative Dna Damage Iron Ion Ros Escherichia Coli Ahpcf Kateg Mutants
Department[Chen, Zhilan ; Zhou, Qiaohong ; Tian, Yun ; Liu, Biyun ; Zhang, Yongyuan ; Wu, Zhenbin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Chen, Zhilan ; Zou, Dandan] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China ; [Zou, Dandan ; Tian, Yun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1016/j.chemosphere.2015.04.076
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000356740900049
WOS KeywordDISINFECTION BY-PRODUCTS ; HYDROXYL RADICAL FORMATION ; HYDROGEN-PEROXIDE ; BLADDER-CANCER ; SUPEROXIDE-DISMUTASE ; DRINKING-WATER ; CHLORINATED PHENOLS ; SENSITIVE DETECTION ; FENTON REACTIONS ; HALOBENZOQUINONES
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23930
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, QH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhilan,Zhou, Qiaohong,Zou, Dandan,et al. Chloro-benzoquinones cause oxidative DNA damage through iron-mediated ROS production in Escherichia coli[J]. CHEMOSPHERE,2015,135:379-386.
APA Chen, Zhilan.,Zhou, Qiaohong.,Zou, Dandan.,Tian, Yun.,Liu, Biyun.,...&Zhou, QH .(2015).Chloro-benzoquinones cause oxidative DNA damage through iron-mediated ROS production in Escherichia coli.CHEMOSPHERE,135,379-386.
MLA Chen, Zhilan,et al."Chloro-benzoquinones cause oxidative DNA damage through iron-mediated ROS production in Escherichia coli".CHEMOSPHERE 135(2015):379-386.
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