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题名: Chloro-benzoquinones cause oxidative DNA damage through iron-mediated ROS production in Escherichia coli
作者: Chen, Zhilan1, 2; Zhou, Qiaohong1; Zou, Dandan2, 3; Tian, Yun1, 3; Liu, Biyun1; Zhang, Yongyuan1; Wu, Zhenbin1
通讯作者: Zhou, QH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
关键词: Chloro-benzoquinones ; Oxidative DNA damage ; Iron ion ; ROS ; Escherichia coli ahpCF katEG mutants
刊名: CHEMOSPHERE
发表日期: 2015-09-01
DOI: 10.1016/j.chemosphere.2015.04.076
卷: 135, 页:379-386
收录类别: SCI
文章类型: Article
部门归属: [Chen, Zhilan ; Zhou, Qiaohong ; Tian, Yun ; Liu, Biyun ; Zhang, Yongyuan ; Wu, Zhenbin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Chen, Zhilan ; Zou, Dandan] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China ; [Zou, Dandan ; Tian, Yun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
英文摘要: Chloro-benzoquinones (CBQs) are a group of disinfection byproducts that are suspected to be potentially carcinogenic. Here, the mechanism of DNA damage caused by CBQs in the presence of ferrous ions was investigated in an Escherichia coil wild type M5 strain and a mutant L5 (ahpCF katEG mutant) strain that carried an enhanced green fluorescent protein reporter under the control of a SOS response gene (recA) promoter. All tested CBQs (including para-benzoquinone, 2-chloro-para-benzoquinone, and dichloro-para-benzoquinones with different substitutes) caused substantial oxidative DNA damage with EC50 values in the micromolar range. Moreover, 2,5-dichloro-para-benzoquinone (2,5-DCBQ), a typical CBQ caused substantial ROS production in Escherichia coil mutant cells. And ROS scavengers provided partial protective effects on genotoxicity of 2,5-DCBQ to Escherichia coil mutant cells. The addition of Fe2+ to the 2,5-DCBQ exposure system caused an increase in DNA oxidative damage; iron-chelating agents could partially prevent these cells from DNA damage. Finally, intracellular AhpCF, catalase E, and catalase G were all found to play an important role in the survival of E. coil cells exposed to CBQs, as indicated by an increased sensitivity of the ahpCF katEG mutant L5 strain to treatment compared with wild type M5 cells. Taken together, these results suggest that CBQs cause oxidative DNA damage in Escherichia coil cells through the participation of iron-mediated ROS production. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: DISINFECTION BY-PRODUCTS ; HYDROXYL RADICAL FORMATION ; HYDROGEN-PEROXIDE ; BLADDER-CANCER ; SUPEROXIDE-DISMUTASE ; DRINKING-WATER ; CHLORINATED PHENOLS ; SENSITIVE DETECTION ; FENTON REACTIONS ; HALOBENZOQUINONES
语种: 英语
WOS记录号: WOS:000356740900049
ISSN号: 0045-6535
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23930
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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