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题名: Targeted Gene Disruption in Zebrafish Reveals Noncanonical Functions of LH Signaling in Reproduction
作者: Chu, Lianhe1; Li, Jianzhen1; Liu, Yun1; Hu, Wei3; Cheng, Christopher H. K.1
通讯作者: Cheng, CHK (reprint author), Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China.
刊名: MOLECULAR ENDOCRINOLOGY
发表日期: 2014-11-01
DOI: 10.1210/me.2014-1061
卷: 28, 期:11, 页:1785-1795
收录类别: SCI
文章类型: Article
部门归属: [Chu, Lianhe ; Li, Jianzhen ; Liu, Yun ; Cheng, Christopher H. K.] Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China ; Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China ; [Hu, Wei] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
英文摘要: The pivotal role of gonadotropin signaling in regulating gonadal development and functions has attracted much research attention in the past 2 decades. However, the precise physiological role of gonadotropin signaling is still largely unknown in fish. In this study, we have established both LH beta-subunit (lhb) and LH receptor (lhr) knockout zebrafish lines by transcription activator-like effector nucleases. Intriguingly, both homozygous lhb and lhr mutant male fish are fertile. The fertilization rate, sperm motility, and histological structure of the testis were not affected in either lhb or lhr mutant males. On the contrary, homozygous lhb mutant females are infertile, whereas homozygous lhr mutant females are fertile. Folliculogenesis was not affected in either lhb or lhr mutants, but oocyte maturation and ovulation were disrupted in lhb mutant, whereas only ovulation was affected in lhr mutant. Differential expression of genes in the ovary involved in steroidogenesis, oocyte maturation, and ovulation was found between the lhb and lhr mutants. These data demonstrate the essential role of LH signaling in oocyte maturation and ovulation, and support the notion that LH acts through the FSH receptor in the absence of LH receptor. Moreover, the defects of lhb mutant could be partially restored by administration of human chorionic gonadotropin. This in vivo evidence in the present study demonstrates, for the first time in any vertebrate species, that LH signaling is indispensable in female reproduction but not in male reproduction. LH signaling is demonstrated to control oocyte maturation and ovulation in the ovary.
关键词[WOS]: FOLLICLE-STIMULATING-HORMONE ; CATFISH CLARIAS-GARIEPINUS ; TROUT ONCORHYNCHUS-MYKISS ; ACTIVIN REGULATORY SYSTEM ; GONADOTROPINS GTH-I ; LUTEINIZING-HORMONE ; SEXUAL-MATURATION ; KNOCKOUT MICE ; DANIO-RERIO ; ONTOGENIC EXPRESSION
语种: 英语
WOS记录号: WOS:000346837100004
ISSN号: 0888-8809
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23921
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
2.Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China
3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
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