IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Targeted Gene Disruption in Zebrafish Reveals Noncanonical Functions of LH Signaling in Reproduction
Chu, Lianhe1; Li, Jianzhen1; Liu, Yun1; Hu, Wei3; Cheng, Christopher H. K.1; Cheng, CHK (reprint author), Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China.
2014-11-01
Source PublicationMOLECULAR ENDOCRINOLOGY
ISSN0888-8809
Volume28Issue:11Pages:1785-1795
AbstractThe pivotal role of gonadotropin signaling in regulating gonadal development and functions has attracted much research attention in the past 2 decades. However, the precise physiological role of gonadotropin signaling is still largely unknown in fish. In this study, we have established both LH beta-subunit (lhb) and LH receptor (lhr) knockout zebrafish lines by transcription activator-like effector nucleases. Intriguingly, both homozygous lhb and lhr mutant male fish are fertile. The fertilization rate, sperm motility, and histological structure of the testis were not affected in either lhb or lhr mutant males. On the contrary, homozygous lhb mutant females are infertile, whereas homozygous lhr mutant females are fertile. Folliculogenesis was not affected in either lhb or lhr mutants, but oocyte maturation and ovulation were disrupted in lhb mutant, whereas only ovulation was affected in lhr mutant. Differential expression of genes in the ovary involved in steroidogenesis, oocyte maturation, and ovulation was found between the lhb and lhr mutants. These data demonstrate the essential role of LH signaling in oocyte maturation and ovulation, and support the notion that LH acts through the FSH receptor in the absence of LH receptor. Moreover, the defects of lhb mutant could be partially restored by administration of human chorionic gonadotropin. This in vivo evidence in the present study demonstrates, for the first time in any vertebrate species, that LH signaling is indispensable in female reproduction but not in male reproduction. LH signaling is demonstrated to control oocyte maturation and ovulation in the ovary.
SubtypeArticle
Department[Chu, Lianhe ; Li, Jianzhen ; Liu, Yun ; Cheng, Christopher H. K.] Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China ; Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China ; [Hu, Wei] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
DOI10.1210/me.2014-1061
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000346837100004
WOS KeywordFOLLICLE-STIMULATING-HORMONE ; CATFISH CLARIAS-GARIEPINUS ; TROUT ONCORHYNCHUS-MYKISS ; ACTIVIN REGULATORY SYSTEM ; GONADOTROPINS GTH-I ; LUTEINIZING-HORMONE ; SEXUAL-MATURATION ; KNOCKOUT MICE ; DANIO-RERIO ; ONTOGENIC EXPRESSION
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23921
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorCheng, CHK (reprint author), Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China.
Affiliation1.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
2.Northwest Normal Univ, Coll Life Sci, Lanzhou, Peoples R China
3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Chu, Lianhe,Li, Jianzhen,Liu, Yun,et al. Targeted Gene Disruption in Zebrafish Reveals Noncanonical Functions of LH Signaling in Reproduction[J]. MOLECULAR ENDOCRINOLOGY,2014,28(11):1785-1795.
APA Chu, Lianhe,Li, Jianzhen,Liu, Yun,Hu, Wei,Cheng, Christopher H. K.,&Cheng, CHK .(2014).Targeted Gene Disruption in Zebrafish Reveals Noncanonical Functions of LH Signaling in Reproduction.MOLECULAR ENDOCRINOLOGY,28(11),1785-1795.
MLA Chu, Lianhe,et al."Targeted Gene Disruption in Zebrafish Reveals Noncanonical Functions of LH Signaling in Reproduction".MOLECULAR ENDOCRINOLOGY 28.11(2014):1785-1795.
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