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Fish MAVS is involved in RLR pathway-mediated IFN response
Zhang, Jun; Zhang, Yi-Bing; Wu, Min; Wang, Bing; Chen, Chen; Gui, Jian-Fang; Zhang, YB (reprint author), Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2014-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume41Issue:2Pages:222-230
AbstractMammalian mitochondrial antiviral signaling protein (MAVS) is an essential adapter involved in retinoic acid-inducible gene-I (RIG-I)-like receptor (RLR)-triggered interferon (IFN) antiviral immunity. Fish MAVS homologues have been identified in several fish species; however, the signaling pathway involving fish MAVS-mediated IFN response remains to be investigated. In the present study, we report identification of a fish MAVS orthologue from crucian carp Carassius auratus blastulae embryonic (CAB) cells and its function role in fish RLR signaling. Crucian carp MAVS is constitutively expressed in CAB cells and is not transcriptionally induced by cytosolic poly (I:C) and IFN. Overexpression of crucian carp MAVS results in activation of fish IFN promoter and ISRE-containing promoter as well as transcriptional expression of IFN and ISGs including PKR and Mx1, which is impaired by functional blockade of signaling molecules TBK1 and IRF3/7. Either cytosolic poly (I:C)-induced or RIG-I-induced IFN response is attenuated by functional blockade of crucian carp MAVS. These results together indicate that fish MAVS contributes to IFN antiviral immunity downstream of cytosolic poly (I:C) and RIG-I and upstream of TBK1 and IRF3/7. Moreover, we provide evidence that apart from crucian carp MAVS, crucian carp MITA is also involved in cytosolic poly (I:C)- and RIG-I-induced IFN response. (C) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordCrucian Carp Carassius Auratus Mavs Mita Rlr Signaling Pathway Ifn Response
Department[Zhang, Jun ; Zhang, Yi-Bing ; Wu, Min ; Wang, Bing ; Chen, Chen ; Gui, Jian-Fang] Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.fsi.2014.09.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordINTERFERON ANTIVIRAL RESPONSE ; INNATE IMMUNE-RESPONSES ; I-LIKE RECEPTORS ; NF-KAPPA-B ; RIG-I ; CARASSIUS-AURATUS ; ADAPTER PROTEIN ; FUNCTIONAL-CHARACTERIZATION ; SIGNALING PROTEIN ; TELEOST FISH
WOS IDWOS:000347017500013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23920
Collection鱼类生物学及渔业生物技术研究中心
Corresponding AuthorZhang, YB (reprint author), Chinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Univ Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jun,Zhang, Yi-Bing,Wu, Min,et al. Fish MAVS is involved in RLR pathway-mediated IFN response[J]. FISH & SHELLFISH IMMUNOLOGY,2014,41(2):222-230.
APA Zhang, Jun.,Zhang, Yi-Bing.,Wu, Min.,Wang, Bing.,Chen, Chen.,...&Zhang, YB .(2014).Fish MAVS is involved in RLR pathway-mediated IFN response.FISH & SHELLFISH IMMUNOLOGY,41(2),222-230.
MLA Zhang, Jun,et al."Fish MAVS is involved in RLR pathway-mediated IFN response".FISH & SHELLFISH IMMUNOLOGY 41.2(2014):222-230.
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