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MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors
Chen, Wen Qin1,2; Hu, Yi Wei1,3; Zou, Peng Fei1; Ren, Shi Si1,3; Nie, Pin1; Chang, Ming Xian1; Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
2015-03-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume49Issue:1Pages:19-30
AbstractThe mitochondrial antiviral signaling protein (MAVS) plays a key role in the signal transduction of RIG-I-like receptors (RLRs)-mediated antiviral response. In the present study, zebrafish MAVS transcript variants, namely MAVS_tv1 and MAVS_tv2, were cloned from zebrafish embryos. The putative MAVS_tv1 protein (full length form) contains an N-terminal CARD domain, a central proline region, and a C-terminal transmembrane domain (TM). MAVS_tv2 is generated by a 190 bp intron fragment insertion. The putative MAVS_tv2 protein lacked TM domain due to a frame shift, with the N-terminal 303 aa residues identical to MAVS_tv1, and no sequence homology for the C-terminal 41 aa residues. Real-time PCR showed that the expression of MAVS_tv1 in ZF4 cells was higher than that of MAVS_tv2, and MAVS variants were induced by Edwardsiella tarda and SVCV infection during the early time points of infection, whereas MAVS_tv1 unchanged or MAVS_tv2 decreased at a later time point after the infection, respectively. Overexpression of MAVS_tv1 and MAVS_tv2 in fish cells conferred antiviral resistance, and activated zebrafish IFN1 and IFN3 promoters. MAVS_tv1 overexpression induced a slow (48 hpf) increased expression of IFNI, mxa, mxb, mxe and RSAD2. In contrast, MAVS tv2 overexpression increased rapidly and transiently the expression of IFN1, IFN2, IFN3, mxc and rsad2 at 6 or 24 hpf. The simultaneous overexpression of MAVS variants and RIG-I in zebrafish embryos led to an accumulative induction of IFNs and IFN-stimulated genes including IFN1, IFN4, mxc, mxe and rsad. Furthermore, MAVS_tv1 cooperated with RIG-I in the accumulation of RIG-I transcript in a positive feedback loop; MAVS_tv2 synergized with MDA5 in the accumulation of MAVS_tv2 transcript. Collectively, these data suggest the molecular mechanisms of fish MAVS variants in antiviral immunity. (C) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordZebrafish Mavs Variants Rig-i-like Receptors Antiviral Activity Svcv
Department[Chen, Wen Qin ; Hu, Yi Wei ; Zou, Peng Fei ; Ren, Shi Si ; Nie, Pin ; Chang, Ming Xian] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Chen, Wen Qin] Hubei Vocat Coll Biotechnol, Wuhan 430070, Hubei Province, Peoples R China ; [Hu, Yi Wei ; Ren, Shi Si] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
DOI10.1016/j.dci.2014.10.017
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000348889800003
WOS KeywordNF-KAPPA-B ; ANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE-RESPONSES ; ADAPTER PROTEIN ; ACTIVATION ; IDENTIFICATION ; EVOLUTION ; VIRUS ; IPS-1 ; EXPRESSION
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Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23913
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorChang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Hubei Vocat Coll Biotechnol, Wuhan 430070, Hubei Province, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Chen, Wen Qin,Hu, Yi Wei,Zou, Peng Fei,et al. MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,49(1):19-30.
APA Chen, Wen Qin.,Hu, Yi Wei.,Zou, Peng Fei.,Ren, Shi Si.,Nie, Pin.,...&Chang, MX .(2015).MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,49(1),19-30.
MLA Chen, Wen Qin,et al."MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 49.1(2015):19-30.
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