中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors
作者: Chen, Wen Qin1, 2; Hu, Yi Wei1, 3; Zou, Peng Fei1; Ren, Shi Si1, 3; Nie, Pin1; Chang, Ming Xian1
通讯作者: Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
关键词: Zebrafish ; MAVS variants ; RIG-I-like receptors ; Antiviral activity ; SVCV
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2015-03-01
DOI: 10.1016/j.dci.2014.10.017
卷: 49, 期:1, 页:19-30
收录类别: SCI
文章类型: Article
部门归属: [Chen, Wen Qin ; Hu, Yi Wei ; Zou, Peng Fei ; Ren, Shi Si ; Nie, Pin ; Chang, Ming Xian] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Chen, Wen Qin] Hubei Vocat Coll Biotechnol, Wuhan 430070, Hubei Province, Peoples R China ; [Hu, Yi Wei ; Ren, Shi Si] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
英文摘要: The mitochondrial antiviral signaling protein (MAVS) plays a key role in the signal transduction of RIG-I-like receptors (RLRs)-mediated antiviral response. In the present study, zebrafish MAVS transcript variants, namely MAVS_tv1 and MAVS_tv2, were cloned from zebrafish embryos. The putative MAVS_tv1 protein (full length form) contains an N-terminal CARD domain, a central proline region, and a C-terminal transmembrane domain (TM). MAVS_tv2 is generated by a 190 bp intron fragment insertion. The putative MAVS_tv2 protein lacked TM domain due to a frame shift, with the N-terminal 303 aa residues identical to MAVS_tv1, and no sequence homology for the C-terminal 41 aa residues. Real-time PCR showed that the expression of MAVS_tv1 in ZF4 cells was higher than that of MAVS_tv2, and MAVS variants were induced by Edwardsiella tarda and SVCV infection during the early time points of infection, whereas MAVS_tv1 unchanged or MAVS_tv2 decreased at a later time point after the infection, respectively. Overexpression of MAVS_tv1 and MAVS_tv2 in fish cells conferred antiviral resistance, and activated zebrafish IFN1 and IFN3 promoters. MAVS_tv1 overexpression induced a slow (48 hpf) increased expression of IFNI, mxa, mxb, mxe and RSAD2. In contrast, MAVS tv2 overexpression increased rapidly and transiently the expression of IFN1, IFN2, IFN3, mxc and rsad2 at 6 or 24 hpf. The simultaneous overexpression of MAVS variants and RIG-I in zebrafish embryos led to an accumulative induction of IFNs and IFN-stimulated genes including IFN1, IFN4, mxc, mxe and rsad. Furthermore, MAVS_tv1 cooperated with RIG-I in the accumulation of RIG-I transcript in a positive feedback loop; MAVS_tv2 synergized with MDA5 in the accumulation of MAVS_tv2 transcript. Collectively, these data suggest the molecular mechanisms of fish MAVS variants in antiviral immunity. (C) 2014 Elsevier Ltd. All rights reserved.
关键词[WOS]: NF-KAPPA-B ; ANTIVIRAL SIGNALING PROTEIN ; INNATE IMMUNE-RESPONSES ; ADAPTER PROTEIN ; ACTIVATION ; IDENTIFICATION ; EVOLUTION ; VIRUS ; IPS-1 ; EXPRESSION
语种: 英语
WOS记录号: WOS:000348889800003
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23913
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors.pdf(3309KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Hubei Vocat Coll Biotechnol, Wuhan 430070, Hubei Province, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Wen Qin]'s Articles
[Hu, Yi Wei]'s Articles
[Zou, Peng Fei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Wen Qin]‘s Articles
[Hu, Yi Wei]‘s Articles
[Zou, Peng Fei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace