IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain
Song, Y.1,2; Duan, X.1,2; Chen, J.1; Huang, W.1; Zhu, Z.1; Hu, W.1; Hu, W (reprint author), 7 Donghu South Rd, Wuhan 430072, Peoples R China.
2015-03-01
Source PublicationJOURNAL OF NEUROENDOCRINOLOGY
ISSN0953-8194
Volume27Issue:3Pages:198-211
AbstractKisspeptin is a neuroendocrine hormone with a critical role in the activation of gonadotrophin-releasing hormone (GnRH) neurones, which is vital for the onset of puberty in mammals. However, the functions of kisspeptin neurones in non-mammalian vertebrates are not well understood. We have used transgenics to labell kisspeptin neurones (Kiss1 and Kiss2) with mCherry in zebrafish (Danio rerio). In kiss1:mCherry transgenic zebrafish, Kiss1 cells were located in the dorsomedial and ventromedial habenula, with their nerve fibres contributing to the fasciculus retroflexus and projecting to the ventral parts of the interpeduncular and raphe nuclei. In kiss2:mCherry zebrafish, Kiss2 cells were primarily located in the dorsal zone of the periventricular hypothalamus and, to a lesser extent, in the periventricular nucleus of the posterior tuberculum and the preoptic area. Kiss2 fibres formed a wide network projecting into the telencephalon, the mesencephalon, the hypothalamus and the pituitary. To study the relationship of kisspeptin neurones and GnRH3 neurones, these fish were crossed with gnrh3:EGFP zebrafish to obtain kiss1:mCherry/gnrh3:EGFP and kiss2:mCherry/gnrh3:EGFP double transgenic zebrafish. The GnRH3 fibres ascending to the habenula were closely associated with Kiss1 fibres projecting from the ventral habenula. On the other hand, GnRH3 fibres and Kiss2 fibres were adjacent but scarcely in contact with each other in the telencephalon and the hypothalamus. The Kiss2 and GnRH3 fibres in the ventral hypothalamus projected into the pituitary via the pituitary stalk. In the pituitary, Kiss2 fibres were directly in contact with GnRH3 fibres in the pars distalis. These results reveal the pattern of kisspeptin neurones and their connections with GnRH3 neurones in the brain, suggesting distinct mechanisms for Kiss1 and Kiss2 in regulating reproductive events in zebrafish.
SubtypeArticle
KeywordKiss1 Kiss2 Gnrh3 Transgenic Zebrafish
Department[Song, Y. ; Duan, X. ; Chen, J. ; Huang, W. ; Zhu, Z. ; Hu, W.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Song, Y. ; Duan, X.] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1111/jne.12251
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism ; Neurosciences & Neurology
WOS SubjectEndocrinology & Metabolism ; Neurosciences
WOS IDWOS:000350146800003
WOS KeywordMETASTASIS-SUPPRESSOR GENE ; PROTEIN-COUPLED RECEPTOR ; MEDAKA ORYZIAS-LATIPES ; GNRH NEURONS ; REPRODUCTIVE-BEHAVIOR ; KISS2 NEURONS ; MIDBRAIN TARGET ; MESSENGER-RNA ; SYSTEM ; EXPRESSION
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23907
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, W (reprint author), 7 Donghu South Rd, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Song, Y.,Duan, X.,Chen, J.,et al. The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain[J]. JOURNAL OF NEUROENDOCRINOLOGY,2015,27(3):198-211.
APA Song, Y..,Duan, X..,Chen, J..,Huang, W..,Zhu, Z..,...&Hu, W .(2015).The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain.JOURNAL OF NEUROENDOCRINOLOGY,27(3),198-211.
MLA Song, Y.,et al."The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain".JOURNAL OF NEUROENDOCRINOLOGY 27.3(2015):198-211.
Files in This Item: Download All
File Name/Size DocType Version Access License
The Distribution of (1817KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Song, Y.]'s Articles
[Duan, X.]'s Articles
[Chen, J.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Song, Y.]'s Articles
[Duan, X.]'s Articles
[Chen, J.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Song, Y.]'s Articles
[Duan, X.]'s Articles
[Chen, J.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: The Distribution of Kisspeptin (Kiss)1-and Kiss2-Positive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.