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Higher antiviral response of RIG-I through enhancing RIG-I/MAVS-mediated signaling by its long insertion variant in zebrafish
Zou, Peng Fei1,2; Chang, Ming Xian2; Li, Ying3; Zhang, Shu Huan2; Fu, Jian Ping2; Chen, Shan Nan2; Nie, Pin2; Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2015-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume43Issue:1Pages:13-24
AbstractAs an intracellular pattern recognition receptor (PRR), the retinoic acid-inducible gene-I (RIG-I) is responsible for the recognition of cytosolic viral nucleic acids and the production of type I interferons (IFNs). In the present study, an insertion variant of RIG-I with 38 amino acids inserted in the N-terminal CARD2 domain, as well as the typical type, named as RIG-Ia and RIG-Ib respectively were identified in zebrafish. RIG-la and RIG-Ib were all up-regulated following the infection of a negative ssRNA virus, the Spring Viremia of Carp Virus (SVCV), and an intracellular Gram-negative bacterial pathogen Edwardsiella tarda, indicating the RLR may have a role in the recognition of both viruses and bacteria. The overexpression of RIG-Ib in cultured fish cells resulted in significant increase in type I IFN promoter activity, and in protection against SVCV infection, whereas the over-expression of RIG-Ia had no direct effect on IFN activation nor antiviral response. Furthermore, it was revealed that both RIG-Ia and RIG-Ib were associated with the downstream molecular mitochondrial antiviral signaling protein, MAVS, and interestingly RIG-Ia when co-transfected with RIG-Ib or MAVS, induced a significantly higher level of type IFN promoter activity and the expression level of Mx and IRF7, implying that the RIG-la may function as an enhancer in the RIG-Ib/MAVS-mediated signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordRetinoic Acid-inducible Gene-i Variant Type i Ifn Production Mitochondrial Antiviral Signaling Protein Rig-lb/mavs-mediated Signaling Pathway
Department[Zou, Peng Fei] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China ; [Zou, Peng Fei ; Chang, Ming Xian ; Zhang, Shu Huan ; Fu, Jian Ping ; Chen, Shan Nan ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Li, Ying] Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China
DOI10.1016/j.fsi.2014.12.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000349731400002
WOS KeywordINNATE IMMUNE-RESPONSE ; INDUCIBLE GENE-I ; PATHOGEN RECOGNITION ; VIRAL-INFECTION ; RNA VIRUSES ; MDA5 ; RECEPTORS ; ACTIVATION ; LGP2 ; IDENTIFICATION
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Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23906
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorChang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
3.Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Zou, Peng Fei,Chang, Ming Xian,Li, Ying,et al. Higher antiviral response of RIG-I through enhancing RIG-I/MAVS-mediated signaling by its long insertion variant in zebrafish[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(1):13-24.
APA Zou, Peng Fei.,Chang, Ming Xian.,Li, Ying.,Zhang, Shu Huan.,Fu, Jian Ping.,...&Chang, MX .(2015).Higher antiviral response of RIG-I through enhancing RIG-I/MAVS-mediated signaling by its long insertion variant in zebrafish.FISH & SHELLFISH IMMUNOLOGY,43(1),13-24.
MLA Zou, Peng Fei,et al."Higher antiviral response of RIG-I through enhancing RIG-I/MAVS-mediated signaling by its long insertion variant in zebrafish".FISH & SHELLFISH IMMUNOLOGY 43.1(2015):13-24.
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