IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish
Chen, Xin1; Mei, Jie1; Wu, Junjie1; Jing, Jing1; Ma, Wenge1; Zhang, Jin1; Dan, Cheng2; Wang, Weimin1; Gui, Jian-Fang1,2; Mei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Agr, Wuhan 430070, Peoples R China.
2015-04-01
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Volume17Issue:2Pages:190-198
AbstractSex dimorphic growth pattern has significant theory and application implications in fish. Recently, a Y- and X-specific allele marker-assisted sex control technique has been developed for mass production of all-male population in yellow catfish (Pelteobagrus fulvidraco), but the genetic information for sex determination and sex control breeding has remained unclear. Here, we attempted to provide the first insight into a comprehensive transcriptome covering multiple tissues from XX females, XY males, and YY super-males of yellow catfish by using 454 GS-FLX platform, for a better assembly and gene coverage. A total of 1,202,933 high quality reads (about 540 Mbp) were obtained and assembled into 28,297 contigs and 141,951 singletons. BLASTX searches against the NCBI non-redundant protein database (nr) led a total of 52,564 unique sequences including 18,748 contigs and 33,816 singletons to match 25,669 known or predicted unique proteins. All of them with annotated function were categorized by gene ontology (GO) analysis, and 712 were assigned to reproduction and reproductive process. Some potential genes relevant to reproductive system including steroid hormone biosynthesis and GnRH (gonadotropin-releasing hormone) signaling pathway were further identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis; and at least 21 sex determination and differentiation-related genes, such as Dmrt1, Sox9a/b, Cyp19b, WT1, and AMH were identified and characterized. Additionally, a total of 82,794 simple sequence repeats (SSRs), 26,450 single nucleotide polymorphisms (SNPs), and 4,145 insertions and deletions (INDELs) were revealed from the transcriptome data. Therefore, the current transcriptome resources highlight further studies on sex-control breeding in yellow catfish and will benefit future studies on reproduction and sex determination in teleost fish.
SubtypeArticle
KeywordYellow Catfish Transcriptome Sex Determination/differentiation All-male Fish Super-male Fish Ssr/snp
Department[Chen, Xin ; Mei, Jie ; Wu, Junjie ; Jing, Jing ; Ma, Wenge ; Zhang, Jin ; Wang, Weimin ; Gui, Jian-Fang] Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Agr, Wuhan 430070, Peoples R China ; [Dan, Cheng ; Gui, Jian-Fang] Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1007/s10126-014-9607-7
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000350555000008
WOS KeywordPELTEOBAGRUS-FULVIDRACO RICHARDSON ; TILAPIA OREOCHROMIS-NILOTICUS ; EXPRESSION CHARACTERIZATION ; SPOTTED GROUPER ; RAINBOW-TROUT ; GIBEL CARP ; KEY-FACTOR ; DIFFERENTIATION ; FISH ; DMRT1
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23905
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorMei, J (reprint author), Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Agr, Wuhan 430070, Peoples R China.
Affiliation1.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Key Lab Freshwater Anim Breeding, Coll Fisheries,Minist Agr, Wuhan 430070, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Xin,Mei, Jie,Wu, Junjie,et al. A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish[J]. MARINE BIOTECHNOLOGY,2015,17(2):190-198.
APA Chen, Xin.,Mei, Jie.,Wu, Junjie.,Jing, Jing.,Ma, Wenge.,...&Mei, J .(2015).A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish.MARINE BIOTECHNOLOGY,17(2),190-198.
MLA Chen, Xin,et al."A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish".MARINE BIOTECHNOLOGY 17.2(2015):190-198.
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