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Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces
Chen, Rong1; Qi, Jianxun2; Yuan, Hongyu1; Wu, Yanan1; Hu, Wei3; Xia, Chun1,4; Xia, C (reprint author), China Agr Univ, Coll Vet Med, Dept Microbiol & Immunol, Beijing 100094, Peoples R China.
2015-03-01
Source PublicationJOURNAL OF STRUCTURAL BIOLOGY
ISSN1047-8477
Volume189Issue:3Pages:259-268
AbstractShort-chain pentraxins (PTXs), including CRP and SAP, are innate pattern recognition receptors that play vital roles in the recognition and elimination of various pathogenic bacteria by triggering the classical complement pathway through C1q. Similar to antibodies, pentraxins can also activate opsonisation and phagocytosis by interacting with Fc receptors (FcRs). Various structural studies on human PTXs have been performed, but there are no reports about the crystal structure of bony fish pentraxins. Here, the crystal structures of zebrafish PTX (Dare-PTX-Ca and Dare-PTX) are presented. Both Dare-PTX-Ca and Dare-PTX are cyclic trimers, which are new forms of crystallised pentraxins. The structures reveal that the ligand-binding pocket (LBP) in the recognition face of Dare-PTX is deep and narrow. Homology modelling shows that LBPs from different Dare-PTX loci differ in shape, reflecting their specific recognition abilities. Furthermore, in comparison with the structure of hCPR, a new C1q binding mode was identified in Dare-PTX. In addition, the FcR-binding sites of hSAP are partially conserved in Dare-PTX. These results will shed light on the understanding of a primitive PTX in bony fish, which evolved approximately 450 million years ago. (C) 2015 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordPentraxin Trimer Zebrafish Structure Evolution
Department[Chen, Rong ; Yuan, Hongyu ; Wu, Yanan ; Xia, Chun] China Agr Univ, Coll Vet Med, Dept Microbiol & Immunol, Beijing 100094, Peoples R China ; [Qi, Jianxun] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol Immunol CASPMI, Beijing 100101, Peoples R China ; [Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Xia, Chun] China Agr Univ, Coll Vet Med, Minist Agr, Key Lab Anim Epidemiol & Zoonosis, Beijing 100094, Peoples R China
DOI10.1016/j.jsb.2015.01.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS KeywordC-REACTIVE PROTEIN ; AMYLOID-P COMPONENT ; CARP CYPRINUS-CARPIO ; SITE-DIRECTED MUTAGENESIS ; SAP-LIKE PENTRAXIN ; MAXIMUM-LIKELIHOOD ; COMPLEMENT-SYSTEM ; C1Q-BINDING SITE ; LIMULUS SAP ; X-RAY
WOS IDWOS:000351249500011
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23902
Collection鱼类生物学及渔业生物技术研究中心
Corresponding AuthorXia, C (reprint author), China Agr Univ, Coll Vet Med, Dept Microbiol & Immunol, Beijing 100094, Peoples R China.
Affiliation1.China Agr Univ, Coll Vet Med, Dept Microbiol & Immunol, Beijing 100094, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol Immunol CASPMI, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
4.China Agr Univ, Coll Vet Med, Minist Agr, Key Lab Anim Epidemiol & Zoonosis, Beijing 100094, Peoples R China
Recommended Citation
GB/T 7714
Chen, Rong,Qi, Jianxun,Yuan, Hongyu,et al. Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces[J]. JOURNAL OF STRUCTURAL BIOLOGY,2015,189(3):259-268.
APA Chen, Rong.,Qi, Jianxun.,Yuan, Hongyu.,Wu, Yanan.,Hu, Wei.,...&Xia, C .(2015).Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces.JOURNAL OF STRUCTURAL BIOLOGY,189(3),259-268.
MLA Chen, Rong,et al."Crystal structures for short-chain pentraxin from zebrafish demonstrate a cyclic trimer with new recognition and effector faces".JOURNAL OF STRUCTURAL BIOLOGY 189.3(2015):259-268.
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