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Identification and Functional Characterization of the Novel Edwardsiella tarda Effector EseJ
Xie, Hai-Xia1; Lu, Jin-Fang1,2; Zhou, Ying1,2; Yi, Jia1,2; Yu, Xiu-Jun3; Leung, Ka Yin4,5; Nie, Pin1; Yu, XJ (reprint author), Univ London Imperial Coll Sci Technol & Med, Microbiol Sect, MRC Ctr Mol Bacteriol & Infect, London, England.
2015-04-01
Source PublicationINFECTION AND IMMUNITY
ISSN0019-9567
Volume83Issue:4Pages:1650-1660
AbstractEdwardsiella tarda is a Gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. The type III secretion system (T3SS) of E. tarda has been identified as a key virulence factor that contributes to pathogenesis in fish. However, little is known about the associated effectors translocated by this T3SS. In this study, by comparing the profile of secreted proteins of the wild-type PPD130/91 and its T3SS ATPase Delta esaN mutant, we identified a new effector by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. This effector consists of 1,359 amino acids, sharing high sequence similarity with Orf29/30 of E. tarda strain EIB202, and is renamed EseJ. The secretion and translocation of EseJ depend on the T3SS. A Delta eseJ mutant strain adheres to epithelioma papillosum of carp (EPC) cells 3 to 5 times more extensively than the wild-type strain does. EseJ inhibits bacterial adhesion to EPC cells from within bacterial cells. Importantly, the Delta eseJ mutant strain does not replicate efficiently in EPC cells and fails to replicate in J774A. 1 macrophages. In infected J774A. 1 macrophages, the Delta eseJ mutant elicits higher production of reactive oxygen species than wild-type E. tarda. The replication defect is consistent with the attenuation of the Delta eseJ mutant in the blue gourami fish model: the 50% lethal dose (LD50) of the Delta eseJ mutant is 2.34 times greater than that of the wild type, and the Delta eseJ mutant is less competitive than the wild type in mixed infection. Thus, EseJ represents a novel effector that contributes to virulence by reducing bacterial adhesion to EPC cells and facilitating intracellular bacterial replication.
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Department[Xie, Hai-Xia ; Lu, Jin-Fang ; Zhou, Ying ; Yi, Jia ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China ; [Lu, Jin-Fang ; Zhou, Ying ; Yi, Jia] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Yu, Xiu-Jun] Univ London Imperial Coll Sci Technol & Med, Microbiol Sect, MRC Ctr Mol Bacteriol & Infect, London, England ; [Leung, Ka Yin] Trinity Western Univ, Fac Nat & Appl Sci, Dept Biol, Langley, BC, Canada ; [Leung, Ka Yin] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
DOI10.1128/IAI.02566-14
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Infectious Diseases
WOS SubjectImmunology ; Infectious Diseases
WOS IDWOS:000351231600041
WOS KeywordIII SECRETION SYSTEM ; ENTERICA SEROVAR TYPHIMURIUM ; REACTIVE OXYGEN ; INTRACELLULAR REPLICATION ; 2-COMPONENT SYSTEM ; EPITHELIAL-CELLS ; VIRULENCE GENES ; TRANSLOCON ; PROTEINS ; PATHOGENESIS
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Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23899
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorYu, XJ (reprint author), Univ London Imperial Coll Sci Technol & Med, Microbiol Sect, MRC Ctr Mol Bacteriol & Infect, London, England.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei Province, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ London Imperial Coll Sci Technol & Med, Microbiol Sect, MRC Ctr Mol Bacteriol & Infect, London, England
4.Trinity Western Univ, Fac Nat & Appl Sci, Dept Biol, Langley, BC, Canada
5.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Xie, Hai-Xia,Lu, Jin-Fang,Zhou, Ying,et al. Identification and Functional Characterization of the Novel Edwardsiella tarda Effector EseJ[J]. INFECTION AND IMMUNITY,2015,83(4):1650-1660.
APA Xie, Hai-Xia.,Lu, Jin-Fang.,Zhou, Ying.,Yi, Jia.,Yu, Xiu-Jun.,...&Yu, XJ .(2015).Identification and Functional Characterization of the Novel Edwardsiella tarda Effector EseJ.INFECTION AND IMMUNITY,83(4),1650-1660.
MLA Xie, Hai-Xia,et al."Identification and Functional Characterization of the Novel Edwardsiella tarda Effector EseJ".INFECTION AND IMMUNITY 83.4(2015):1650-1660.
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