IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)
Feng, Xiu1,2; Yu, Xiaomu1; Pang, Meixia1,2; Liu, Haiyang1,2; Tong, Jingou1; Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2015-04-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume182Pages:37-46
AbstractSomatostatins (SSs) are a structurally diverse family of peptides that play important roles in the regulation of growth, development and metabolism in vertebrates. In this study, three preprosomatostatin genes (PSSs) in the common carp, Cyprinus carpio (Cc) were identified and characterized. Based on cloned sequences and genome BLAST, six isoforms of the PSS gene in C. carpio (CcPSS) were identified and included CcPSS1a and CcPSS1b, CcPSS2a and CcPSS2b, and finally, CcPSS3a and CcPSS3b. The open reading frames (ORF) of CcPSS1a, CcPSS2a and CcPSS3a consist of 345, 336 and 363 nucleotides. During embryonic development, the expressions of CcPSS2 and CcPSS3 were first observed at the stage of optic vesicle, and CcPSS1 mRNA was initially detected at the stage of muscular effect. The highest mRNA levels of CcPSS1, CcPSS2 and CcPSS3 were observed at 1-day post-hatch (dph), 2-dph and the stage of heart beating, respectively. In the adult brain, the distributions of three CcPSS mRNAs were differential but overlapping in the hypothalamus, telencephalon and medulla oblongata. For peripheral tissues, all three CcPSS mRNAs were detected in the mid-intestine, and CcPSS1 and CcPSS3 mRNAs were also expressed in the liver. Owing to the importance of somatostatins on regulating growth, functional mutations of CcPSSs were identified in a C carpio population. A total of 23 polymorphic sites were detected in CcPSS1a and CcPSS3a. Of them, two SNPs (CcPSS1a-g.922C>T, and CcPSS3a-g.1125C>A) were significantly associated with growth traits, indicating their potential applications in gene (marker)-assisted selective breeding in C. carpio. (C) 2014 Elsevier Inc All rights reserved.
SubtypeArticle
KeywordCyprinus Carpio Preprosomatostatin Molecular Characterization Expression Pattern Genetic Association
Department[Feng, Xiu ; Yu, Xiaomu ; Pang, Meixia ; Liu, Haiyang ; Tong, Jingou] Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Feng, Xiu ; Pang, Meixia ; Liu, Haiyang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1016/j.cbpb.2014.12.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000352746300005
WOS KeywordQUANTITATIVE TRAIT LOCI ; MESSENGER-RNAS ; SOMATOSTATIN PRECURSORS ; EMBRYONIC-DEVELOPMENT ; POLYGENIC EXPRESSION ; CARCASS COMPOSITION ; PROSOMATOSTATIN-I ; HORMONE RECEPTOR ; FISH ; CORTISTATIN
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23897
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JO (reprint author), Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Feng, Xiu,Yu, Xiaomu,Pang, Meixia,et al. Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2015,182:37-46.
APA Feng, Xiu,Yu, Xiaomu,Pang, Meixia,Liu, Haiyang,Tong, Jingou,&Tong, JO .(2015).Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,182,37-46.
MLA Feng, Xiu,et al."Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 182(2015):37-46.
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