| Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio) | |
| Feng, Xiu1,2; Yu, Xiaomu1; Pang, Meixia1,2; Liu, Haiyang1,2; Tong, Jingou1; Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. | |
| 2015-04-01 | |
| Source Publication | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| ISSN | 1096-4959 |
| Volume | 182Pages:37-46 |
| Abstract | Somatostatins (SSs) are a structurally diverse family of peptides that play important roles in the regulation of growth, development and metabolism in vertebrates. In this study, three preprosomatostatin genes (PSSs) in the common carp, Cyprinus carpio (Cc) were identified and characterized. Based on cloned sequences and genome BLAST, six isoforms of the PSS gene in C. carpio (CcPSS) were identified and included CcPSS1a and CcPSS1b, CcPSS2a and CcPSS2b, and finally, CcPSS3a and CcPSS3b. The open reading frames (ORF) of CcPSS1a, CcPSS2a and CcPSS3a consist of 345, 336 and 363 nucleotides. During embryonic development, the expressions of CcPSS2 and CcPSS3 were first observed at the stage of optic vesicle, and CcPSS1 mRNA was initially detected at the stage of muscular effect. The highest mRNA levels of CcPSS1, CcPSS2 and CcPSS3 were observed at 1-day post-hatch (dph), 2-dph and the stage of heart beating, respectively. In the adult brain, the distributions of three CcPSS mRNAs were differential but overlapping in the hypothalamus, telencephalon and medulla oblongata. For peripheral tissues, all three CcPSS mRNAs were detected in the mid-intestine, and CcPSS1 and CcPSS3 mRNAs were also expressed in the liver. Owing to the importance of somatostatins on regulating growth, functional mutations of CcPSSs were identified in a C carpio population. A total of 23 polymorphic sites were detected in CcPSS1a and CcPSS3a. Of them, two SNPs (CcPSS1a-g.922C>T, and CcPSS3a-g.1125C>A) were significantly associated with growth traits, indicating their potential applications in gene (marker)-assisted selective breeding in C. carpio. (C) 2014 Elsevier Inc All rights reserved. |
| Subtype | Article |
| Keyword | Cyprinus Carpio Preprosomatostatin Molecular Characterization Expression Pattern Genetic Association |
| Department | [Feng, Xiu ; Yu, Xiaomu ; Pang, Meixia ; Liu, Haiyang ; Tong, Jingou] Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Feng, Xiu ; Pang, Meixia ; Liu, Haiyang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| DOI | 10.1016/j.cbpb.2014.12.001 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Zoology |
| WOS Subject | Biochemistry & Molecular Biology ; Zoology |
| WOS ID | WOS:000352746300005 |
| WOS Keyword | QUANTITATIVE TRAIT LOCI ; MESSENGER-RNAS ; SOMATOSTATIN PRECURSORS ; EMBRYONIC-DEVELOPMENT ; POLYGENIC EXPRESSION ; CARCASS COMPOSITION ; PROSOMATOSTATIN-I ; HORMONE RECEPTOR ; FISH ; CORTISTATIN |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/23897 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Feng, Xiu,Yu, Xiaomu,Pang, Meixia,et al. Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2015,182:37-46. |
| APA | Feng, Xiu,Yu, Xiaomu,Pang, Meixia,Liu, Haiyang,Tong, Jingou,&Tong, JO .(2015).Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,182,37-46. |
| MLA | Feng, Xiu,et al."Molecular characterization and expression of three preprosomatostatin genes and their association with growth in common carp (Cyprinus carpio)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 182(2015):37-46. |
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