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题名: Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus
作者: Gao, X. C.1; Chen, Z. Y.1; Yuan, J. D.1; Zhang, Q. Y.1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Andrias davidianus ranavirus ; Chinese giant salamander spleen cell ; electron microscopy ; viromatrix
刊名: JOURNAL OF COMPARATIVE PATHOLOGY
发表日期: 2015-02-01
DOI: 10.1016/j.jcpa.2015.01.001
卷: 152, 期:2-3, 页:110-113
收录类别: SCI
文章类型: Article
部门归属: [Gao, X. C. ; Chen, Z. Y. ; Yuan, J. D. ; Zhang, Q. Y.] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Pathology ; Veterinary Sciences
研究领域[WOS]: Pathology ; Veterinary Sciences
英文摘要: Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe disease in Chinese giant salamanders, the largest extant amphibian in the world. A fish cell line, Epithelioma papulosum cyprinid (EPC), and a new amphibian cell line, Chinese giant salamander spleen cell (GSSC), were infected with ADRV and observed by light and electron microscopy. The morphological changes in these two cell lines infected with ADRV were compared. Cytopathic effect (CPE) began with rounding of the cells, progressing to cell detachment in the cell monolayer, followed by cell lysis. Significant CPE was visualized as early as 24 h post infection (hpi) in EPC cells and at 36 hpi in GSSC cells. Microscopical examination showed clear and significant CPE in EPC cells, while less extensive and irregular CPE with some adherent cells remaining was observed in GSSC cells. Following ADRV infection, CPE became more extensive. Transmission electron micrographs showed many virus particles around cytoplasmic vacuoles, formed as crystalline arrays or scattered in the cytoplasm of infected cells. Infected cells showed alteration in nuclear morphology, with condensed and marginalized nuclear chromatin on the inner aspect of the nuclear membrane and formation of a cytoplasmic viromatrix adjacent to the nucleus in both cell lines. Some virus particles were also detected in the nucleus of infected GSSC cells. Both cell lines are able to support replication of ADRV and can therefore be used to investigate amphibian ranaviruses. (C) 2015 Elsevier Ltd. All rights reserved.
关键词[WOS]: IRIDOVIRUS ; MORPHOGENESIS ; GROUPER ; VIRUS
语种: 英语
WOS记录号: WOS:000354001900004
ISSN号: 0021-9975
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23895
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
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