IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus
Gao, X. C.; Chen, Z. Y.; Yuan, J. D.; Zhang, Q. Y.; Zhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2015-02-01
Source PublicationJOURNAL OF COMPARATIVE PATHOLOGY
ISSN0021-9975
Volume152Issue:2-3Pages:110-113
AbstractAndrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe disease in Chinese giant salamanders, the largest extant amphibian in the world. A fish cell line, Epithelioma papulosum cyprinid (EPC), and a new amphibian cell line, Chinese giant salamander spleen cell (GSSC), were infected with ADRV and observed by light and electron microscopy. The morphological changes in these two cell lines infected with ADRV were compared. Cytopathic effect (CPE) began with rounding of the cells, progressing to cell detachment in the cell monolayer, followed by cell lysis. Significant CPE was visualized as early as 24 h post infection (hpi) in EPC cells and at 36 hpi in GSSC cells. Microscopical examination showed clear and significant CPE in EPC cells, while less extensive and irregular CPE with some adherent cells remaining was observed in GSSC cells. Following ADRV infection, CPE became more extensive. Transmission electron micrographs showed many virus particles around cytoplasmic vacuoles, formed as crystalline arrays or scattered in the cytoplasm of infected cells. Infected cells showed alteration in nuclear morphology, with condensed and marginalized nuclear chromatin on the inner aspect of the nuclear membrane and formation of a cytoplasmic viromatrix adjacent to the nucleus in both cell lines. Some virus particles were also detected in the nucleus of infected GSSC cells. Both cell lines are able to support replication of ADRV and can therefore be used to investigate amphibian ranaviruses. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordAndrias Davidianus Ranavirus Chinese Giant Salamander Spleen Cell Electron Microscopy Viromatrix
Department[Gao, X. C. ; Chen, Z. Y. ; Yuan, J. D. ; Zhang, Q. Y.] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.jcpa.2015.01.001
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaPathology ; Veterinary Sciences
WOS SubjectPathology ; Veterinary Sciences
WOS IDWOS:000354001900004
WOS KeywordIRIDOVIRUS ; MORPHOGENESIS ; GROUPER ; VIRUS
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23895
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Gao, X. C.,Chen, Z. Y.,Yuan, J. D.,et al. Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus[J]. JOURNAL OF COMPARATIVE PATHOLOGY,2015,152(2-3):110-113.
APA Gao, X. C.,Chen, Z. Y.,Yuan, J. D.,Zhang, Q. Y.,&Zhang, QY .(2015).Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus.JOURNAL OF COMPARATIVE PATHOLOGY,152(2-3),110-113.
MLA Gao, X. C.,et al."Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus".JOURNAL OF COMPARATIVE PATHOLOGY 152.2-3(2015):110-113.
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