| Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus | |
| Gao, X. C.; Chen, Z. Y.; Yuan, J. D.; Zhang, Q. Y.; Zhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. | |
| 2015-02-01 | |
| Source Publication | JOURNAL OF COMPARATIVE PATHOLOGY
 |
| ISSN | 0021-9975 |
| Volume | 152Issue:2-3Pages:110-113 |
| Abstract | Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe disease in Chinese giant salamanders, the largest extant amphibian in the world. A fish cell line, Epithelioma papulosum cyprinid (EPC), and a new amphibian cell line, Chinese giant salamander spleen cell (GSSC), were infected with ADRV and observed by light and electron microscopy. The morphological changes in these two cell lines infected with ADRV were compared. Cytopathic effect (CPE) began with rounding of the cells, progressing to cell detachment in the cell monolayer, followed by cell lysis. Significant CPE was visualized as early as 24 h post infection (hpi) in EPC cells and at 36 hpi in GSSC cells. Microscopical examination showed clear and significant CPE in EPC cells, while less extensive and irregular CPE with some adherent cells remaining was observed in GSSC cells. Following ADRV infection, CPE became more extensive. Transmission electron micrographs showed many virus particles around cytoplasmic vacuoles, formed as crystalline arrays or scattered in the cytoplasm of infected cells. Infected cells showed alteration in nuclear morphology, with condensed and marginalized nuclear chromatin on the inner aspect of the nuclear membrane and formation of a cytoplasmic viromatrix adjacent to the nucleus in both cell lines. Some virus particles were also detected in the nucleus of infected GSSC cells. Both cell lines are able to support replication of ADRV and can therefore be used to investigate amphibian ranaviruses. (C) 2015 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Andrias Davidianus Ranavirus Chinese Giant Salamander Spleen Cell Electron Microscopy Viromatrix |
| Department | [Gao, X. C. ; Chen, Z. Y. ; Yuan, J. D. ; Zhang, Q. Y.] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| DOI | 10.1016/j.jcpa.2015.01.001 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Pathology ; Veterinary Sciences |
| WOS Subject | Pathology ; Veterinary Sciences |
| WOS ID | WOS:000354001900004 |
| WOS Keyword | IRIDOVIRUS ; MORPHOGENESIS ; GROUPER ; VIRUS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/23895 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Zhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| Affiliation | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Gao, X. C.,Chen, Z. Y.,Yuan, J. D.,et al. Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus[J]. JOURNAL OF COMPARATIVE PATHOLOGY,2015,152(2-3):110-113. |
| APA | Gao, X. C.,Chen, Z. Y.,Yuan, J. D.,Zhang, Q. Y.,&Zhang, QY .(2015).Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus.JOURNAL OF COMPARATIVE PATHOLOGY,152(2-3),110-113. |
| MLA | Gao, X. C.,et al."Morphological Changes in Amphibian and Fish Cell Lines Infected with Andrias davidianus Ranavirus".JOURNAL OF COMPARATIVE PATHOLOGY 152.2-3(2015):110-113. |
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