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Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection
Jiang, Yao1,3; He, Libo1; Ju, Changsong1,2; Pei, Yongyan1,3; Ji, Myonghuan2; Li, Yongming1; Liao, Lanjie1; Jang, Songhun1,2; Zhu, Zuoyan1; Wang, Yaping1; Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2015-05-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume44Issue:1Pages:88-99
AbstractToll-like receptor 5 (TLR5), a member of Toll-like receptors (TLRs) family and is responsible for the bacterial flagellin recognition in vertebrates, play an important role in innate immunity. In the study, two TLR5 genes of grass carp (Ctenopharyngodon idellus), named CiTLR5a and CiTLR5b, were cloned and analyzed. Both CiTLR5a and CiTLR5b are typical TLR proteins, including LRR motif, transmembrane region and TIR domain. The full-length cDNA of CiTLR5a is 3054 bp long, with a 2646 bp open reading frame (ORF), 78 bp 5' untranslated regions (UTR), and 330 bp 3' UTR. The full-length cDNA of CiTLR5b is 3326 bp, with a 2627 bp ORF, 95 bp 5' UTR, and 594 bp 3' UTR. Phylogenetic analysis showed that CiTLR5a and CiTLR5b were closed to the TLR5 of cirrhinus mrigala, cyprinus_carpio, and danio redo. Subcellular localization indicated that CiTLR5a and CiTLR5b shared similar localization pattern and may locate in the plasma membrane of transfected cells. Real-time quantitative PCR revealed CiTLR5a and CiTLR5b were constitutively expressed in all examined tissues, whereas the highest expressed tissue differed. Following exposure to flagellin and GCRV, CiTLR5a and CiTLR5b were up-regulated significantly. Moreover, the downstream genes of TLR5 signal pathway such as MyD88, NF-kappa B, IRF7, IL-1 beta, and TNF-alpha also up-regulated significantly, whereas the I kappa B gene was down-regulated, suggesting that CiTLR5a and CiTLR5b involved in response to flagellin stimulation and GCRV infection. The results obtained in the study would provide a new insight for further understand the function of TLR5 in teleost fish. (C) 2015 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGrass Carp Toll-like Receptor 5 Flagellin Gcrv
Department[Jiang, Yao ; He, Libo ; Ju, Changsong ; Pei, Yongyan ; Li, Yongming ; Liao, Lanjie ; Jang, Songhun ; Zhu, Zuoyan ; Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Ju, Changsong ; Ji, Myonghuan ; Jang, Songhun] Kim Il Song Univ, Coll Life Sci, Dept Zool, Pyongyang, South Korea ; [Jiang, Yao ; Pei, Yongyan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1016/j.fsi.2015.01.024
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000353743900011
WOS KeywordBACTERIAL FLAGELLIN ; SIGNALING PATHWAYS ; MOLECULAR-CLONING ; INNATE IMMUNITY ; BONY FISH ; IDENTIFICATION ; TLR5 ; RNA ; CANDIDATE ; PROFILES
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23894
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Kim Il Song Univ, Coll Life Sci, Dept Zool, Pyongyang, South Korea
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Yao,He, Libo,Ju, Changsong,et al. Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(1):88-99.
APA Jiang, Yao.,He, Libo.,Ju, Changsong.,Pei, Yongyan.,Ji, Myonghuan.,...&Wang, YP .(2015).Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection.FISH & SHELLFISH IMMUNOLOGY,44(1),88-99.
MLA Jiang, Yao,et al."Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection".FISH & SHELLFISH IMMUNOLOGY 44.1(2015):88-99.
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