IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp salmonicida
Long, Meng1,2; Zhao, Juan3; Li, Tongtong1,2; Tafalla, Carolina4; Zhang, Qianqian1,2; Wang, Xiehao1,2; Gong, Xiaoning1; Shen, Zhixin3; Li, Aihua1; Shen, ZX (reprint author), Qinghai Prov Fishery Environm Monitoring Ctr, Xining 810000, Peoples R China.
2015-06-03
Source PublicationJOURNAL OF PROTEOMICS
ISSN1874-3919
Volume122Pages:41-54
AbstractFurunculosis caused by Aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids, including rainbow trout (Oncorhynchus mykiss). However, the immune mechanisms that are elicited in rainbow trout against the invasion of A. salmonicida are not yet fully understood. In this study, we examined the spleen to investigate the immune response of rainbow trout at 3 days post-infection by A. salmonicida at the transcriptome and proteome levels by using Illumina-seq and iTRAQ methods, respectively. A total of 1036 genes and 133 proteins were found to undergo differential expression during the immune response of the spleen against A. salmonicida infection. Gene ontology and KEGG analysis were conducted among the differentially expressed genes and proteins, revealing that immune system process and response to stimulus were the top two biological processes, and immune system, signaling molecules and interaction, and immune diseases were the differential pathways activated. Correlation analysis of transcriptomic and proteomic results showed 17 proteins (11 upregulated and 6 downregulated) having consistent expression at RNA and protein levels. Moreover, protein protein interaction analysis showed that diseases, proteasome, aminoacyl-tRNA biosynthesis, and nucleotide metabolism were the main interactions among the consistently expressed proteins. Consequently, these upregulated proteins, namely, ferritin, CD209, IL13R alpha 1, VDAC2, GIMAP7, PSMA1, and two ANXA11s could be considered as potential biomarkers for rainbow trout immune responses.
SubtypeArticle
KeywordRainbow Trout A. sA.monicida Furunculosis Spleen Transcriptome Proteome
Department[Long, Meng ; Li, Tongtong ; Zhang, Qianqian ; Wang, Xiehao ; Gong, Xiaoning ; Li, Aihua] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Long, Meng ; Li, Tongtong ; Zhang, Qianqian ; Wang, Xiehao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Zhao, Juan ; Shen, Zhixin] Qinghai Prov Fishery Environm Monitoring Ctr, Xining 810000, Peoples R China ; [Tafalla, Carolina] Ctr Invest Sanidad Anim CISAINIA, Madrid 28130, Spain
DOI10.1016/j.jprot.2015.03.031
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000355710600004
WOS KeywordDIFFERENTIALLY EXPRESSED GENES ; EEL ANGUILLA-JAPONICA ; DC-SIGN ; SALMO-SALAR ; QUANTITATIVE PROTEOMICS ; RESPONSIVE PROTEINS ; MOLECULAR-CLONING ; ANTIBODY RESPONSE ; DENDRITIC CELLS ; VIRUS-INFECTION
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23891
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorShen, ZX (reprint author), Qinghai Prov Fishery Environm Monitoring Ctr, Xining 810000, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qinghai Prov Fishery Environm Monitoring Ctr, Xining 810000, Peoples R China
4.Ctr Invest Sanidad Anim CISAINIA, Madrid 28130, Spain
Recommended Citation
GB/T 7714
Long, Meng,Zhao, Juan,Li, Tongtong,et al. Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp salmonicida[J]. JOURNAL OF PROTEOMICS,2015,122:41-54.
APA Long, Meng.,Zhao, Juan.,Li, Tongtong.,Tafalla, Carolina.,Zhang, Qianqian.,...&Shen, ZX .(2015).Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp salmonicida.JOURNAL OF PROTEOMICS,122,41-54.
MLA Long, Meng,et al."Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp salmonicida".JOURNAL OF PROTEOMICS 122(2015):41-54.
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