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题名: Transcriptomic and proteomic analyses of splenic immune mechanisms of rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida subsp salmonicida
作者: Long, Meng1, 2; Zhao, Juan3; Li, Tongtong1, 2; Tafalla, Carolina4; Zhang, Qianqian1, 2; Wang, Xiehao1, 2; Gong, Xiaoning1; Shen, Zhixin3; Li, Aihua1
通讯作者: Shen, ZX (reprint author), Qinghai Prov Fishery Environm Monitoring Ctr, Xining 810000, Peoples R China.
关键词: Rainbow trout ; A. salmonicida ; Furunculosis ; Spleen ; Transcriptome ; Proteome
刊名: JOURNAL OF PROTEOMICS
发表日期: 2015-06-03
DOI: 10.1016/j.jprot.2015.03.031
卷: 122, 页:41-54
收录类别: SCI
文章类型: Article
部门归属: [Long, Meng ; Li, Tongtong ; Zhang, Qianqian ; Wang, Xiehao ; Gong, Xiaoning ; Li, Aihua] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Long, Meng ; Li, Tongtong ; Zhang, Qianqian ; Wang, Xiehao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Zhao, Juan ; Shen, Zhixin] Qinghai Prov Fishery Environm Monitoring Ctr, Xining 810000, Peoples R China ; [Tafalla, Carolina] Ctr Invest Sanidad Anim CISAINIA, Madrid 28130, Spain
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Furunculosis caused by Aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids, including rainbow trout (Oncorhynchus mykiss). However, the immune mechanisms that are elicited in rainbow trout against the invasion of A. salmonicida are not yet fully understood. In this study, we examined the spleen to investigate the immune response of rainbow trout at 3 days post-infection by A. salmonicida at the transcriptome and proteome levels by using Illumina-seq and iTRAQ methods, respectively. A total of 1036 genes and 133 proteins were found to undergo differential expression during the immune response of the spleen against A. salmonicida infection. Gene ontology and KEGG analysis were conducted among the differentially expressed genes and proteins, revealing that immune system process and response to stimulus were the top two biological processes, and immune system, signaling molecules and interaction, and immune diseases were the differential pathways activated. Correlation analysis of transcriptomic and proteomic results showed 17 proteins (11 upregulated and 6 downregulated) having consistent expression at RNA and protein levels. Moreover, protein protein interaction analysis showed that diseases, proteasome, aminoacyl-tRNA biosynthesis, and nucleotide metabolism were the main interactions among the consistently expressed proteins. Consequently, these upregulated proteins, namely, ferritin, CD209, IL13R alpha 1, VDAC2, GIMAP7, PSMA1, and two ANXA11s could be considered as potential biomarkers for rainbow trout immune responses.
关键词[WOS]: DIFFERENTIALLY EXPRESSED GENES ; EEL ANGUILLA-JAPONICA ; DC-SIGN ; SALMO-SALAR ; QUANTITATIVE PROTEOMICS ; RESPONSIVE PROTEINS ; MOLECULAR-CLONING ; ANTIBODY RESPONSE ; DENDRITIC CELLS ; VIRUS-INFECTION
语种: 英语
WOS记录号: WOS:000355710600004
ISSN号: 1874-3919
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23891
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qinghai Prov Fishery Environm Monitoring Ctr, Xining 810000, Peoples R China
4.Ctr Invest Sanidad Anim CISAINIA, Madrid 28130, Spain
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