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题名: Establishment of three cell lines from Chinese giant salamander and their sensitivities to the wild-type and recombinant ranavirus
作者: Yuan, Jiang-Di1; Chen, Zhong-Yuan1; Huang, Xing1; Gao, Xiao-Chan1; Zhang, Qi-Ya1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
刊名: VETERINARY RESEARCH
发表日期: 2015-06-12
DOI: 10.1186/s13567-015-0197-9
卷: 46
收录类别: SCI
文章类型: Article
部门归属: [Yuan, Jiang-Di ; Chen, Zhong-Yuan ; Huang, Xing ; Gao, Xiao-Chan ; Zhang, Qi-Ya] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Veterinary Sciences
研究领域[WOS]: Veterinary Sciences
英文摘要: Known as lethal pathogens, Ranaviruses have been identified in diseased fish, amphibians (including Chinese giant salamander Andrias davidianus, the world's largest amphibian) and reptiles, causing organ necrosis and systemic hemorrhage. Here, three Chinese giant salamander cell lines, thymus cell line (GSTC), spleen cell line (GSSC) and kidney cell line (GSKC) were initially established. Their sensitivities to ranaviruses, wild-type Andrias davidianus ranavirus (ADRV) and recombinant Rana grylio virus carrying EGFP gene (rRGV-EGFP) were tested. Temporal transcription pattern of ranavirus major capsid protein (MCP), fluorescence and electron microscopy observations showed that both the wild-type and recombinant ranavirus could replicate in the cell lines.
关键词[WOS]: RANA-GRYLIO VIRUS ; ANDRIAS-DAVIDIANUS ; AQUACULTURE ANIMALS ; IRIDOVIRUS ; INFECTION ; CULTURE ; EXPRESSION ; DECLINE ; THYMUS ; FROG
语种: 英语
WOS记录号: WOS:000356219600001
ISSN号: 0928-4249
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/23888
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
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文件名: Establishment of three cell lines from Chinese giant salamander and their sensitivities to the wild-type and recombinant ranavirus.pdf
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