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Unraveling the genome structure of cyanobacterial podovirus A-4L with long direct terminal repeats
Ou, Tong; Liao, Xiang-Yong; Gao, Xiao-Chan; Xu, Xu-Dong; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2015-05-04
Source PublicationVIRUS RESEARCH
ISSN0168-1702
Volume203Pages:4-9
AbstractThe freshwater cyanobacterial virus (cyanophage) A-4L, a podovirus, can infect the model cyanobacterium Anabaena sp. strain PCC 7120 resulting in a high burst size and forming concentric plaques on its lawns. The complete genome sequence of A-4L was determined by the combination of high-throughput sequencing, terminal transferase-mediated polymerase chain reaction and restriction mapping. It contains 41,750 bp with 810 bp direct terminal repeats and 38 potential open reading frames. As compared with other cyanobacterial podoviruses in diverse ecosystems, the A-4L has the longest terminal repeat and shares similar genome organizations with freshwater members. Furthermore, phylogenetic analysis based on concatenated sequences of eight core proteins indicated that freshwater cyanobacterial podoviruses were clustered together and distinct from marine counterparts, suggesting a clear divergence in the cyanobacterial podovirus lineage between freshwater and marine ecosystems. Our findings uncover the unique genome structure of A-4L which contains long direct terminal repeats, and create the first model system to address knowledge gaps in understanding cyanobacterial virus-host interactions at the molecular level. (C) 2015 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordCyanobacterial Podovirus A-4l Cyanophage Genomic Analysis Terminal Repeat Cyanobacterial Virus-host Model System
Department[Ou, Tong ; Liao, Xiang-Yong ; Gao, Xiao-Chan ; Xu, Xu-Dong ; Zhang, Qi-Ya] Univ Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
DOI10.1016/j.virusres.2015.03.012
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000356746200002
WOS KeywordFRESH-WATER ; MICROCYSTIS-AERUGINOSA ; PHORMIDIUM-FOVEOLARUM ; MARINE SYNECHOCOCCUS ; PHOTOSYNTHESIS GENES ; CYANOPHAGE ; SEQUENCE ; PHAGE ; ANABAENA ; STRATEGIES
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23887
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
AffiliationUniv Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ou, Tong,Liao, Xiang-Yong,Gao, Xiao-Chan,et al. Unraveling the genome structure of cyanobacterial podovirus A-4L with long direct terminal repeats[J]. VIRUS RESEARCH,2015,203:4-9.
APA Ou, Tong,Liao, Xiang-Yong,Gao, Xiao-Chan,Xu, Xu-Dong,Zhang, Qi-Ya,&Zhang, QY .(2015).Unraveling the genome structure of cyanobacterial podovirus A-4L with long direct terminal repeats.VIRUS RESEARCH,203,4-9.
MLA Ou, Tong,et al."Unraveling the genome structure of cyanobacterial podovirus A-4L with long direct terminal repeats".VIRUS RESEARCH 203(2015):4-9.
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