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Contribution of nuclease to the pathogenesis of Aeromonas hydrophila
Ji, Yachan1; Li, Jinquan2; Qin, Zhendong1; Li, Aihua5; Gu, Zemao1,3,4; Liu, Xiaoling1,3,4; Lin, Li1,3,4; Zhou, Yang1,3,4; Zhou, Y (reprint author), Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan, Peoples R China.
2015-07-04
Source PublicationVIRULENCE
ISSN2150-5594
Volume6Issue:5Pages:515-522
AbstractAeromonas hydrophila is a gram-negative bacterium that is widely distributed in aquatic environments and can cause septicemia in both fish and humans. However, the underlying mechanisms leading to severe infection are not well understood. In this study, an A. hydrophila nuclease (ahn) deletion mutant was constructed to investigate its contribution to pathogenesis. This mutant did not differ from the wild-type strain in terms of its growth or hemolytic phenotype. However, the ahn-deficient mutant was more susceptible to being killed by fish macrophages and mouse blood in vitro. Furthermore, evidence obtained using both fish and murine infection models strongly indicated that the inactivation of Ahn impaired the ability of A. hydrophila to evade innate immune clearance in vivo. More importantly, the virulence of the mutant was attenuated in both fish and mice, with reductions in dissemination capacities and mortality rates. These findings implicate Ahn in A. hydrophila virulence, with important functions in evading innate immune defenses.
SubtypeArticle
KeywordPathogenesis Aeromonas Hydrophila Nuclease
Department[Ji, Yachan ; Qin, Zhendong ; Gu, Zemao ; Liu, Xiaoling ; Lin, Li ; Zhou, Yang] Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan, Peoples R China ; [Li, Jinquan] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan, Peoples R China ; [Gu, Zemao ; Liu, Xiaoling ; Lin, Li ; Zhou, Yang] Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Peoples R China ; [Gu, Zemao ; Liu, Xiaoling ; Lin, Li ; Zhou, Yang] Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan, Peoples R China ; [Li, Aihua] State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
DOI10.1080/21505594.2015.1049806
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Infectious Diseases ; Microbiology
WOS SubjectImmunology ; Infectious Diseases ; Microbiology
WOS IDWOS:000357649400018
WOS KeywordNEUTROPHIL EXTRACELLULAR TRAPS ; GROUP-A STREPTOCOCCUS ; CLINICAL ISOLATE ; VIRULENCE ; IDENTIFICATION ; INFECTION ; EFFECTOR ; MACROPHAGES ; ENTEROTOXIN ; EXPRESSION
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23885
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhou, Y (reprint author), Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan, Peoples R China.
Affiliation1.Huazhong Agr Univ, Dept Aquat Anim Med, Coll Fisheries, Wuhan, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan, Peoples R China
3.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan, Peoples R China
4.Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan, Peoples R China
5.State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Ji, Yachan,Li, Jinquan,Qin, Zhendong,et al. Contribution of nuclease to the pathogenesis of Aeromonas hydrophila[J]. VIRULENCE,2015,6(5):515-522.
APA Ji, Yachan.,Li, Jinquan.,Qin, Zhendong.,Li, Aihua.,Gu, Zemao.,...&Zhou, Y .(2015).Contribution of nuclease to the pathogenesis of Aeromonas hydrophila.VIRULENCE,6(5),515-522.
MLA Ji, Yachan,et al."Contribution of nuclease to the pathogenesis of Aeromonas hydrophila".VIRULENCE 6.5(2015):515-522.
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