IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Mixed mycobacterial infections in farmed sturgeons
Zhang, De Feng1,2; Ji, Cheng3; Zhang, Xu Jie1; Li, Tong Tong1; Li, Ai Hua1; Gong, Xiao Ning1; Li, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2015-08-01
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume46Issue:8Pages:1914-1923
AbstractBased on microbiological and histopathological examinations and DNA sequencing, several outbreaks of mycobacteriosis in the reared sturgeons, including Chinese sturgeon (Acipenser sinensis Gray) and Amur sturgeon (Acipenser schrencki), were identified during 2009 to 2010. Forty-nine isolates of non-tuberculous mycobacteria(NTM)were isolated from 19 diseased sturgeons. In total, seven species of Mycobacterium were identified, namely, Mycobacterium chelonae, Mycobacterium marinum, Mycobacterium gordonae, Mycobacterium fortuitum, Mycobacterium szulgai, Mycobacterium arupense and Mycobacterium porcinum. Among them, M. marinum was found to be more prevalent (89.5%) compared with the other mycobacterial species. When two molecular biological methods, PCR-DGGE (denaturing gradient gel electrophoresis) analysis and rpoB gene library sequencing, were used to analyse the mycobacterial DNAs extracted from the diseased fish tissues, mixed infections of two or three mycobacterial species were found being the predominant infection form (94.7%) in sturgeon mycobacteriosis. M. marinum was the only one species that caused sturgeon mycobacteriosis alone. Virulence assay showed that M. marinum possessed stronger pathogenicity to zebrafish killing 100% of fish in 28days at 10(3) cfu/fish than the other species. These results suggested that M. marinum is the major pathogenic bacteria in sturgeon mycobacteriosis. To the best of our knowledge, this study is the first report on mycobacteriosis in farmed Chinese and Amur sturgeons as well as the first isolation of M. porcinum and M. arupense from fish.
SubtypeArticle
KeywordSturgeon Mixed Infection Mycobacterium Mycobacteriosis
Department[Zhang, De Feng ; Zhang, Xu Jie ; Li, Tong Tong ; Li, Ai Hua ; Gong, Xiao Ning] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Zhang, De Feng] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China ; [Ji, Cheng] Soochow Univ, Sch Basic Med & Biol Sci, Ctr Circadian Clocks, Suzhou, Peoples R China
DOI10.1111/are.12346
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000357820100012
WOS KeywordORNAMENTAL FISH ; SP-NOV. ; AQUARIUM FISH ; SPP. ; IDENTIFICATION ; INFLAMMATION ; AQUACULTURE ; ARUPENSE ; CULTURE ; LEVEL
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/23884
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
3.Soochow Univ, Sch Basic Med & Biol Sci, Ctr Circadian Clocks, Suzhou, Peoples R China
Recommended Citation
GB/T 7714
Zhang, De Feng,Ji, Cheng,Zhang, Xu Jie,et al. Mixed mycobacterial infections in farmed sturgeons[J]. AQUACULTURE RESEARCH,2015,46(8):1914-1923.
APA Zhang, De Feng.,Ji, Cheng.,Zhang, Xu Jie.,Li, Tong Tong.,Li, Ai Hua.,...&Li, AH .(2015).Mixed mycobacterial infections in farmed sturgeons.AQUACULTURE RESEARCH,46(8),1914-1923.
MLA Zhang, De Feng,et al."Mixed mycobacterial infections in farmed sturgeons".AQUACULTURE RESEARCH 46.8(2015):1914-1923.
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